荒漠生物土壤结皮关键细菌的筛选鉴定及生态应用潜力

doi:10.13287/j.1001-9332.202505.011

应用生态学报 ›› 2025, Vol. 36 ›› Issue (5): 1407-1412.doi: 10.13287/j.1001-9332.202505.011

荒漠生物土壤结皮关键细菌的筛选鉴定及生态应用潜力

赵丽娜, 陈笑含, 李明鑫, 李亚宁, 张依妮, 高源婧坤, 古绍彬^*

河南科技大学食品与生物工程学院, 河南洛阳 471000

收稿日期:2024-12-27 修回日期:2025-03-07 出版日期:2025-05-18 发布日期:2025-11-18
通讯作者: *E-mail: shaobingu@haust.edu.cn
作者简介:赵丽娜, 女, 1990年生, 博士, 副教授。主要从事微生物生态学研究。E-mail: zhaolina@haust.edu.cn
基金资助:
国家自然基金青年基金项目(32201383)、河南省重大科技专项项目(231100310200)和河南省重点研发项目(241111314200)

Selection, identification, and ecological application potential of key bacteria in biological soil crusts of deserts

ZHAO Lina, CHEN Xiaohan, LI Mingxin, LI Yaning, ZHANG Yini, GAO Yuanjingkun, GU Shaobin^*

College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 471000, Henan, China

Received:2024-12-27 Revised:2025-03-07 Online:2025-05-18 Published:2025-11-18

摘要/Abstract

摘要： 为解决荒漠化地区土壤养分匮乏和植物生长受限的问题,本研究从生物土壤结皮(BSC)中筛选并鉴定出具有产胞外水解酶和溶磷解钾功能的关键菌株,并评估了它们在逆境条件下的生存能力。结果表明:从BSC中筛选到的13株菌株中,YD-7在淀粉酶和纤维素酶活性上表现最佳,水解圈直径分别为2.27和1.70 cm;YD-8和YD-5在蛋白酶活性上表现最佳,水解圈直径分别为2.79和2.15 cm。在溶磷解钾能力测试中,YD-5、YD-7和YD-8菌株表现出较高的活性,可溶性磷含量分别为43.66、37.23、35.61 mg·L^-1。胁迫条件下的生长试验表明,这些菌株在PEG-6000浓度低于10%、温度为28～36 ℃、pH值4～9以及NaCl浓度低于3%的条件下均能表现出良好的生长特性(存活率＞50%)。系统发育树分析发现,YD-5、YD-7和YD-8分别为溶血性不动杆菌、嗜麦芽寡养单胞菌和肺炎克雷伯菌,均具有荒漠土壤改良潜力。

关键词: 生物土壤结皮, 荒漠化土壤, 微生物筛选, 菌种鉴定

Abstract: To address the challenges of nutrient deficiency and plant growth in desertified areas, we screened and identified key strains with extracellular hydrolase production and phosphorus and potassium solubilizing functions from biological soil crusts (BSC), and evaluated their survival capabilities under stressed conditions. Among the 13 strains screened from BSC, YD-7 exhibited the highest activity for amylase and cellulase, with hydrolysis zone diameters of 2.27 and 1.70 cm, respectively. YD-8 and YD-5 exhibited the highest activity for protease, with hydrolysis zone diameters of 2.79 and 2.15 cm, respectively. In the tests for phosphorus and potassium solubilizing abilities, strains YD-5, YD-7, and YD-8 showed high activity, with soluble phosphorus contents of 43.66, 37.23, and 35.61 mg·L^-1, respectively. Growth experiments under stress conditions indicated that these strains exhibited good growth characteristics (survival rate >50%) under conditions of PEG-6000 concentration below 10%, tempera-tures of 28-36 ℃, pH values of 4-9, and NaCl concentration below 3%. Analysis based on phylogenetic tree revealed that YD-5, YD-7, and YD-8 were identified as Acinetobacter haemolyticus, Stenotrophomonas maltophilia, and Klebsiella pneumoniae, respectively. All the three strains have the potential for improving the quality of desert soils.

Key words: biological soil crust, desertification soil, microbial screening, strain identification

赵丽娜, 陈笑含, 李明鑫, 李亚宁, 张依妮, 高源婧坤, 古绍彬. 荒漠生物土壤结皮关键细菌的筛选鉴定及生态应用潜力[J]. 应用生态学报, 2025, 36(5): 1407-1412.

ZHAO Lina, CHEN Xiaohan, LI Mingxin, LI Yaning, ZHANG Yini, GAO Yuanjingkun, GU Shaobin. Selection, identification, and ecological application potential of key bacteria in biological soil crusts of deserts[J]. Chinese Journal of Applied Ecology, 2025, 36(5): 1407-1412.

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荒漠生物土壤结皮关键细菌的筛选鉴定及生态应用潜力

Selection, identification, and ecological application potential of key bacteria in biological soil crusts of deserts

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