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银离子抗性细菌质粒的分离与鉴定

李钧敏;金则新   

  1. 台州学院生态研究所, 临海 317000
  • 收稿日期:2005-01-31 修回日期:2005-06-19 出版日期:2006-02-18 发布日期:2006-02-18

Isolation and determination of silverresistant bacteria plasmids

LI Junmin;JIN Zexin   

  1. Ecology Institute of Taizhou University,Linhai 317000,China
  • Received:2005-01-31 Revised:2005-06-19 Online:2006-02-18 Published:2006-02-18

摘要: 通过对不同化工厂废水处理池的活性污泥进行富集、不同浓度银离子驯化后,分离得到30株银离子抗性细菌,最大硝酸银耐受量可达80 mg·ml-1.对这些细菌进行质粒抽提与鉴定,质粒检出率为76.67%.40 mmol·L-1的苯甲酸钠对HAg4细菌质粒的消除率可达98.75%;而350 μg· ml-1的吖啶橙对HAg4细菌质粒的消除率只有77.78%.细菌质粒与银离子抗性密切相关.

关键词: 天然林, 赤松, 个体生物量, 林分密度

Abstract: Through the enrichment of the active mud obtained from three chemical plants and the domestication with different concentration Ag+ solution,thirty bacteria strains with silver (Ag+)resistance were isolated,among which,the highest Ag+-resistant concentration was 80 mg·ml-1.The plasmids in these bacteria were extracted,with the detection rate of 76.67%.The elimination rate of the plasmid in HAg4 bacteria was 98.75% by 40 mmol·L-1 sodium benzoate,and 77.78% by 350 μg·ml-1 acridine orange.It was suggested that the Ag+ -resistance of bacteria was highly correlated with their plasmids.

Key words: Natural forest, Pinus densiflora, Individual biomass, Stand density