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三种生物质能源植物组织的乙醇提取液对烟粉虱生物活性的影响

周福才1;周桂生2;李传明1;杨益众1;钦佩3   

  1. 1扬州大学园艺与植物保护学院, 江苏扬州 225009;2扬州大学/江苏省作物遗传生理重点实验室, 江苏扬州 225009;3南京大学盐生植物实验室, 南京 210093
  • 收稿日期:2008-08-29 修回日期:1900-01-01 出版日期:2009-03-20 发布日期:2009-03-20

Effects of alcohol extracts from three kinds of biomass energy plant tissues on biological activity of Bemisia tabaci.

ZHOU Fu-cai1;ZHOU Gui-sheng2;LI Chuan-ming1;YANG Yi-zhong1;QIN Pei3   

  1. 1School of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, Jiangsu, China;2Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, Jiangsu, China;3Halophyte Research Laboratory of Nanjing University, Nanjing 210093,China
  • Received:2008-08-29 Revised:1900-01-01 Online:2009-03-20 Published:2009-03-20

摘要: 为了探讨蓖麻、海滨锦葵等生物质能源植物组织的抽提物作为植物保护剂的可行性,用蓖麻种子、蓖麻叶片、海滨锦葵叶片等生物质能源植物组织的乙醇粗提物处理烟粉虱成虫,4、24、48、72和96 h后测定其体内的谷胱甘肽转移酶和羧酸酯酶活性,并利用Y型嗅觉仪测定烟粉虱对3种生物质能源植物组织的乙醇粗提物的嗅觉反应.结果表明:3种生物质能源植物组织的乙醇粗提物对烟粉虱谷胱甘肽转移酶和羧酸酯酶均有明显影响,随着浓度的提高,其作用增强,其中250倍液的作用与1.8%的阿维菌素3000倍液作用相当.3种提取液的250倍液对烟粉虱成虫具有明显的驱避作用,其中蓖麻种子、蓖麻叶片和海滨锦葵叶片等乙醇粗提物的驱避率分别为100.0%、96.7%和79.4%.表明蓖麻种子、蓖麻叶片和海滨锦葵叶片的乙醇粗抽提物对烟粉虱具有驱避作用和其他生物活性.

关键词: 证据权重法, 综合评价, 沉积物质量评价, 沉积物

Abstract: To test the feasibility of using raw extracts from the tissues of biomass energy plants Ricinus communi and Kosteletzkya virginica as plant protection agents, the alcohol extracts from R. communi seed and leaf and from K. virginica leaf were used to treat adult Bemisia tabaci by spraying. The glutathione S-transferase and carboxylesterase activities in B. tabaci body were measured after treated for 4 h, 24 h, 48 h, 72 h, and 96 h, and the olfaction responses of B. tabaci to the alcohol extracts were detected with a Y-tube olfactomet. All the three alcohol extracts obviously inhibited the glutathione S-transferase and carboxylesterase activities in a concentration-dependent manner. The inhibitory effect of the 250-times diluted alcohol extracts on the two enzyme activities was equivalent to that of 3000 times-diluted 18% avermectins. In addition, the 250-times diluted alcohol extracts had obvious repellent effect on B. tabaci, with the repellent coefficient of the alcohol extracts from R. communi seed and leaf and from K, virginica leaf being 1000%, 967%, and 794%, respectively. All of these suggested that the test three alcohol extracts had repellent and other biological effects on B. tabaci.

Key words: weight of evidence (WOE) approach, integrated assessment, sediment quality assessment, sediment.