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龙须菜对重金属铜胁迫的生理响应

朱喜锋1;邹定辉1,2;简建波1;陈伟洲1;刘慧慧1;杜虹1   

  1. 1汕头大学海洋生物研究所, 广东汕头 515063;2华南理工大学环境科学与工程学院, 广州 510006
  • 收稿日期:2008-10-31 修回日期:1900-01-01 出版日期:2009-06-20 发布日期:2009-06-20

Physiological responses of Gracilaria lemaneiformis to copper stress.

ZHU Xi-feng1;ZOU Ding-hui1,2;JIAN Jian-bo1;CHEN Wei-zhou1;LIU Hui-hui1;DU Hong1   

  1. 1Institute of Marine Biology, Shantou University, Shantou 515063, Guangdong, China;2College of Environmental Science and Engineering, South China University of Technology, Guangzhou 510006, China
  • Received:2008-10-31 Revised:1900-01-01 Online:2009-06-20 Published:2009-06-20

摘要: 研究了大型海藻龙须菜(Gracilaria lemaneiformis)对不同浓度重金属铜(0、25、50、100、250和500 μg·L-1)胁迫的生理响应.结果表明:当Cu2+浓度≥50 μg·L-1时,龙须菜藻体的相对生长速率显著下降,最大光化学量子产量、最大相对电子传递速率和相对电子传递效率呈相同的变化趋势.随着Cu2+浓度的升高,龙须菜藻体最大净光合速率和光饱和点显著降低,而光补偿点显著升高,叶绿素a、类胡萝卜素和藻胆蛋白含量则呈先升高后下降的趋势;当Cu2+浓度达到500 μg·L-1时,叶绿素a、类胡萝卜素和藻胆蛋白含量显著下降.说明龙须菜在低浓度Cu2+胁迫下具有一定的抵抗能力,而当Cu2+浓度≥50 μg·L-1时,会对藻体生理活动造成显著的抑制作用.

关键词: 涡度相关, 雪面蒸发, 潜热, 日变化, 气象因子

Abstract: Gracilaria lemaneiformis was exposed to 0, 25, 50, 100, 250 and 500 μ g·L-1 of Cu2+ to study its physiological responses to Cu2+ st ress. When the Cu2+ concentration was ≥50 μg·L-1, the relative gr owth rate (RGR) of G. lemaneiformis decreased significantly, and the optimal quantum yield (Fv/Fm), the maximum relative electron transfer rate (r ETRmax), and the relative electron transfer efficiency (α) exhibited th e same variation trend, compared with the control. With the increase of Cu2+  concentration, the maximum net photosynthetic rate (Pmax) and l ight saturation point (LSP) decreased significantly, light compensation poin t (LCP) had a significant increase, while chlorophyll a, carotenoid, and phy cobiliprotein contents decreased after an initial increase. When the Cu2+ concentration reached 500 μg·L-1, the chlorophyll a, carotenoid, and phy cobiliprotein contents decreased significantly. It was suggested that G. leman eiformis could tolerate low concentration Cu2+ stress, but its physiolog ical activities were inhibited markedly when exposed to ≥50 μg·L-1 of C u2+.

Key words: eddy covariance, snow  evaporation, latent heat, diurnal variation, meteorological factor.