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长期磷肥处理农田黑土细菌群落特征

白震1,2;张明3;张旭东1;朱平4;彭畅4   

  1. 1中国科学院沈阳应用生态研究所陆地生态过程重点实验室, 沈阳 110016;2中国科学院研究生院, 北京 100039;3辽宁大学生命科学学院, 沈阳 110036;4吉林省农业科学院中国农业科技东北创新中心, 长春 130033
  • 收稿日期:2007-05-09 修回日期:1900-01-01 出版日期:2008-07-20 发布日期:2008-07-20

Characteristics of bacterial community in an arable mollisol under longterm phosphorus fertilization.

BAI Zhen1,2;ZHANG Ming3;ZHANG Xu-dong1;ZHU Ping4;PENG Chang4   

  1. 1Key Laboratory of Terrestrial Ecological Process, Institute of Applied
    Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2Graduate University of Chinese Academy of Sciences, Beijing 100039, China; 3College of Life Science, Liaoning University, Shenyang 110036, China; 4Chinese Research Center of Northeast Agricultural Science and Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
  • Received:2007-05-09 Revised:1900-01-01 Online:2008-07-20 Published:2008-07-20

摘要: 研究了公主岭国家黑土长期定位站不同施肥处理(对照、磷、氮磷、氮磷钾、有机肥-磷、有机肥-氮-磷、有机肥-氮-磷-钾等)土壤基本理化性质及细菌群落特征.结果表明:有机肥处理土壤有机质(OM)、总氮(TN)、有效磷(AP)、碱解氮(AN)、速效钾(AK)等养分含量显著增加,而单施化肥各处理OM、AN含量无明显变化.与未施肥对照处理相比,有机肥-氮-磷配施与有机肥-氮-磷-钾配施处理总细菌磷脂脂肪酸分别增加34%和62%,G+菌磷脂脂肪酸分别增加58%和74%,G-菌磷脂脂肪酸分别增加64%和69%.各化肥处理细菌脂肪酸含量均有所下降,以磷处理最低.除G+菌磷脂脂肪酸外(有机肥-磷配施显著大于氮-磷-钾肥配施),氮-磷肥配施、氮-磷-钾肥配施与有机肥-磷肥配施三者间细菌脂肪酸含量无显著差异.相关分析表明,各细菌磷脂脂肪酸与OM、AP、AN、AK、NO3--N含量呈极显著相关.

关键词: 春玉米, 积温, RuBP羧化酶, PEP羧化酶, 光合速率, 叶绿素

Abstract: Soil samples were taken from the Gongzhuling national long-term experimental station in Jilin Province of Northeast China to study their basic physical and chemical properties and the characteristics of bacterial community under different fertilization systems, i.e., CK, P, N-P, N-P-K, manure-P, manure-N-P, and manure-N-P-K. The results showed that applying manure increased the contents of soil organic matter (OM), total nitrogen (TN), available phosphorus (AP), alkalihydrolysable nitrogen (AN) and available potassium (AK) significantly, while applying chemical fertilizers had no significant effects on the contents of soil OM and AN. Comparing with CK, the total bacterial phospholipid fatty acids, G+ bacterial phospholipid fatty acids, and G- bacterial phospholipid fatty acids in treatments manure-N-P and manure-N-P-K increased by 34% and 62%, 58% and 74%, and 64% and 69%, respectively. Applying chemical fertilizers, especially P, decreased the bacterial phospholipid fatty acids. Except G+ bacterial phospholipid fatty acids whose content was significantly higher in treatment manure-P than in treatment N-P-K, the bacterial phospholipid fatty acids had no significant difference between treatments N-P, N-P-K and manure-P. There existed significant correlations between various groups of bacterial phospholipid fatty acids and soil OM, AN, AP, AK, and NO3--N .

Key words: spring maize, accumulated temperature, RuBPCase, PEPCase, photosynthetic rate, chlorophyll.