关键词: 小麦, 水分胁迫, 蛋白表达, 生理特性, 耕作措施, 杂草, 生物多样性, 竞争, 燕麦产量

Abstract: With drought-resistant wheat (Triticum aestivum) cultivar Changw
u 134 and drought-sensitive cultivar Zhengyin 1 as test materials, and by using
-12 MPa PEG 6000 to treat their seeds, this paper studied the protein express
ion and physiological properties of the sprouts under different soil moisture co
nditions. SDS-PAGE analysis showed that water stress induced the production of
two new proteins with molecular weights of 395 kDa and 230 kDa in Changwu
134 but not in Zhengyin 1 sprouts. Under normal water supply, the expression of
the protein with molecular weight of 485 kDa in the sprouts of both Changwu 13
4 and Zhengyin 1 increased with sprout growth. This protein was preliminarily na
med as water-sensitive protein, due to its water-sensitivity and of newly disc
overed protein. The determinations of physiological properties showed that under
 water stress, the sprouts of drought-resistant Changwu 134 had higher root/sho
ot ratio and higher relative water content, but lower relative membrane permeabi
lity and lower malondlaldehyde content than those of drought-sensitive Zhengyin
 1.

Key words: wheat, water stress, protein expression, physiological property, tillage, weed, biodiversity, competition, oat yield.