关键词: 硝普钠, NO₃^- 胁迫, 黄瓜幼苗, 抗氧化酶, 生长, 微卫星, 转录组, 高通量测序, 分子标记, 实蝇

Abstract: In this study, cucumber seedlings were cultivated in nutrient solution added with different concentration (0.05, 0.1, 0.2, and 0.3 mmol·L^-1) sodium nitroprusside (SNP) as nitric oxide (NO) donor to study the effects of exogenous NO on the growth of cucumber seedlings and the activities of antioxidant enzymes in cucumber leaves under NO₃^- stress. Under the stress of 140 mmol·L^-1 NO₃^- ,treating with 0.1 mmol·L^-1 of SNP for 1 d or 7 d increased the leaf soluble protein content and superoxidase (SOD), catalase (CAT), and ascorbic acid peroxidase (APX) activities, and decreased the leaf malondialdehyde (MDA) content significantly, suggesting that exogenous NO could enhance the capacity of cucumber seedlings in scavenging active oxygen species, protect the seedlings from the peroxidation of membrane lipids, and promote the seedlings growth and increase their resistance to high concentration NO₃^- stress. After the cucumber seedlings grew in 0.3 mmol·L^-1 of SNP for 7 d, the activities of leaf SOD, POD, and CAT decreased, and the MDA content increased, resulting in the injury of cucumber seedlings. It was indicated that certain concentration (0.1-0.2 mmol·L^-1) exogenous NO could alleviate the NO₃^- stress to cucumber seedlings.

Key words: sodium nitroprusside, NO₃^- stress, cucumber seedlings, antioxidant enzyme, growth, microsatellite, transcriptome, high-throughput sequencing, molecular marker, fruit fly.