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应用生态学报 ›› 2010, Vol. 21 ›› Issue (10): 2666-2670.

• 研究报告 • 上一篇    下一篇

氯化镉胁迫下葡萄根、叶内源一氧化氮和活性氧的生成

邵小杰1,2,3,杨洪强1,2,3   

  1. 1山东农业大学园艺科学与工程学院| 山东泰安 271018|2作物生物学国家重点实验室| 山东泰安 271018|3山东省果树生物学重点实验室| 山东泰安 271018
  • 出版日期:2010-10-18 发布日期:2010-10-18

Endogenous NO and ROS generation in grape roots and leaves under CdCl2 stress.

SHAO Xiao-jie1,2,3, YANG Hong-qiang1,2,3   

  1. 1College of Horticultural Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China|2State Key Laboratory of Crop
     Biology, Tai’an 271018, Shandong, China;3Shandong Province Key Laboratory of Fruit Tree Biology, Tai’an 271018, Shandong, China
  • Online:2010-10-18 Published:2010-10-18

摘要: 以‘泽香’葡萄扦插苗为试材,在水培条件下,研究了氯化镉(CdCl2)处理下葡萄根系和叶片内源一氧化氮(NO)、活性氧(ROS)的生成规律,以及根系丙二醛(MDA)含量与根系活力的变化.结果表明:在0~1 mmol·L-1范围内,随着CdCl2处理浓度的增加, 葡萄扦插苗根系和叶片NO含量、一氧化氮合酶(NOS)活性和根系活力先升高后下降;CdCl2浓度为0.01 mmol·L-1 时,根系NO含量和NOS活性分别提高51%和63%,超过0.1 mmol·L-1时则显著下降;CdCl2浓度为0.01和0.05 mmol·L-1时,叶片NO含量和NOS活性显著提高.0.5和1.0 mmol·L-1的CdCl2处理显著提高了根系O2-·产生速率、H2O2含量和MDA含量;同浓度CdCl2处理下,叶片中O2-·产生速率明显低于根系O2-·,而H2O2含量明显高于根系.

关键词: 葡萄, 氯化镉, 一氧化氮, 活性氧代谢, 京津冀地区, 资源环境承载力, 驱动因子, 状态空间模型, Tobit模型

Abstract: Taking the cutting seedlings of grape variety ‘Ze-xiang’ as test materials, a hydroponic experiment was conducted to study the generation patterns of
endogenous nitric oxide (NO) and reactive oxygen species (ROS) in their roots and leaves, and the changes of root MDA content and root activity under the stress of cadmium chloride (CdCl2). In the treatments 0-1 mmol·L-1 of CdCl2, the NO content and NOS activity in roots and leaves and the root activity all presented a tendency of increasing first and decreasing afterwards with increasing CdCl2. At 0.01 mmol·L-1 of CdCl2, the NO content and NOS activity in roots increased by 51% and 63%, respectively; but at >0.1 mmol·L-1 of CdCl2, they decreased significantly. At 0.01 and 0.05 mmol·L-1 of CdCl2, the leaf NO content and NOS activity had the greatest increase; at 0.5 and 1.0 mmol·L-1 of CdCl2, the root O2-· generation rate and H2O2 and MDA contents increased significantly. At the same concentrations of CdCl2, the O2-·  generation rate in leaves was much lower while the H2O2 content was much higher, compared with those in roots.

Key words: grape, CdCl2, NO, ROS metabolize, BeijingTianjinHebei, resource environmental bearing capacity, driving factors, state space model, Tobit model.