封育草地根系生长与球囊霉素固碳的年限效应

doi:10.13287/j.1001-9332.202608.007

应用生态学报 ›› 2026, Vol. 37 ›› Issue (8): 2647-2654.doi: 10.13287/j.1001-9332.202608.007

封育草地根系生长与球囊霉素固碳的年限效应

陶斯涛^1,2,3, 朱兆龙^1,2,4*, 王宝荣⁵, 郭梁^1,2, 安韶山^1,2,4

¹中国科学院教育部水土保持与生态环境研究中心, 陕西杨凌 712100;
²中国科学院水利部水土保持研究所, 陕西杨凌 712100;
³中国科学院大学, 北京 100049;
⁴西北农林科技大学水土保持与荒漠化整治全国重点实验室, 陕西杨凌 712100;
⁵西北农林科技大学草业与草原学院, 陕西杨凌 712100

收稿日期:2025-12-15 修回日期:2026-06-03 出版日期:2026-08-18 发布日期:2027-02-18
通讯作者: ^*E-mail: zhu_zl@nwafu.edu.cn
作者简介:陶斯涛, 男, 2001年生, 硕士研究生。主要从事土壤微生物固碳研究。E-mail: 1532928715@qq.com
基金资助:
国家自然科学基金项目(42307440,42577400,42507466)、中国博士后基金会博后面上项目(2023M732879)、陕西省博士后特别资助项目(2023BSHTBZZ27)和国家资助博士后研究人员计划项目(GZC20232153)

Temporal effects of grassland enclosure on root growth and glomalin-mediated carbon sequestration

TAO Sitao^1,2,3, ZHU Zhaolong^1,2,4*, WANG Baorong⁵, GUO Liang^1,2, AN Shaoshan^1,2,4

¹Research Center of Soil and Water Conservation and Ecological Environment, Chinese Academy of Sciences and Ministry of Education, Yangling 712100, Shaanxi, China;
²Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, Shaanxi, China;
³University of Chinese Academy of Sciences, Beijing 100049, China;
⁴State Key Laboratory of Soil and Water Conservation and Desertification Control, Northwest A&F University, Yangling 712100, Shaanxi, China;
⁵College of Grassland Science,Northwest A&F University, Yangling 712100, Shaanxi, China

Received:2025-12-15 Revised:2026-06-03 Online:2026-08-18 Published:2027-02-18

摘要/Abstract

摘要： 封育是退化草地修复的关键措施之一,草地恢复后固碳效应的提升直接关系到区域生态质量改善及碳中和目标的实现。以云雾山国家级自然保护区天然牧草地和不同封育年限(5、10、25、30年)草地为研究对象,采用内生长土芯法开展4年原位根系生长(6、12、24、48个月)试验,探究封育草地根系生长与球囊霉素相关土壤蛋白(GRSP)固碳的年限效应。结果表明:在不同封育年限样地中,生长袋内根系生物量随样地封育年限增长而显著增加,在封育25年样地达到峰值(每袋0.36 g),较天然牧草地增长了125.0%。GRSP含量与根系生物量同步增长,在封育25年样地达到最大值(3.16 g·kg^-1)。偏最小二乘路径模型分析显示,根系生物量与GRSP通过促进颗粒态有机碳积累(路径系数分别为0.18和0.33)驱动土壤有机碳固存(路径系数=0.47)。主成分(PC)分析表明,封育25年样地的PC1得分最高,表明该阶段活性碳库积累达到最优。此时该样地土壤有机碳与矿物结合态有机碳含量也最高,证实封育25年是该区域草地碳库积累的关键节点。建议该区域草地封育25年后适度利用,以加快养分循环,进一步提升草地生态系统碳汇潜力。

关键词: 封育草地, 根系生长, 球囊霉素相关土壤蛋白, 土壤有机碳, 内生长土芯法

Abstract: Enclosure is one of the key measures for restoring degraded grasslands. The enhancement of carbon sequestration after grassland restoration is directly related to the improvement of regional ecological quality and the achievement of carbon neutrality goals. We conducted an in-situ root growth experiment lasting for four years (6, 12, 24, and 48 months) using the internal growth core method across natural grassland and grasslands with different closure durations (5, 10, 25, and 30 years) in Yunwushan National Nature Reserve. We explored the duration effect on root growth and globulin-related soil protein (GRSP) carbon sequestration in enclosed grasslands. The results showed that in plots with different closure durations, root biomass in growth bags significantly increased with the duration of closure, reaching a peak (0.36 g per bag) in the 25-year closure plot, which was a 125.0% increase compared to that in the natural grassland. The GRSP content increased simultaneously with the root biomass, reaching a maximum value (3.16 g·kg^-1 ) in the 25-year closure plot. Partial least squares path model analysis revealed that root biomass and GRSP drove soil organic carbon sequestration by promoting the accumulation of particulate organic carbon (path coefficients of 0.18 and 0.33, respectively), which in turn affected soil organic carbon sequestration (path coefficient=0.47). Principal component (PC) analysis indicated that the PC1 score was highest in the 25-year closure plot, suggesting that the accumulation of reactive carbon pool reached its optimal level. At this stage, the soil organic carbon and mineral-bound organic carbon conten were also the highest, confirming that 25 years of closure is a key milestone for carbon pool accumulation. We recommended that grasslands in this region be moderately utilized after 25 years of closure to accelerate nutrient cycling and further enhance the carbon sink potential of the grasslands.

Key words: enclosed grassland, root growth, glomalin-related soil protein, soil organic carbon, ingrowth core method

陶斯涛, 朱兆龙, 王宝荣, 郭梁, 安韶山. 封育草地根系生长与球囊霉素固碳的年限效应[J]. 应用生态学报, 2026, 37(8): 2647-2654.

TAO Sitao, ZHU Zhaolong, WANG Baorong, GUO Liang, AN Shaoshan. Temporal effects of grassland enclosure on root growth and glomalin-mediated carbon sequestration[J]. Chinese Journal of Applied Ecology, 2026, 37(8): 2647-2654.

参考文献

[1] Jia MH, Wang YC, He J, et al. Soil aggregates stability is evidently enhanced by super-binding of the N-terminal disordered tail of glomalin to soil minerals. Soil Biology & Biochemistry, 2025, 210: 109908
[2] 郭子华, 郝欢欢, 马洁, 等. 修复措施对中国退化草地土壤有机碳组分的影响. 应用生态学报,2025,36(11): 3327-3338
[3] He X, Zhang R, Shama A, et al. Dynamic monitoring of grassland land cover types of Inner Mongolia 1990-2023 and testing the causal relationship between meteorological data and grassland area. Geomatics, Natural Hazards and Risk, 2026, 17: 2621845
[4] Buisson E, Archibald S, Fidelis A, et al. Ancient grasslands guide ambitious goals in grassland restoration. Science, 2022, 377: 594-598
[5] 张艺斐, 王兴, 潘海珠, 等. 中国北方荒漠草原长期围栏封育恢复效应及其环境依赖性. 生态学报, 2025, 45(17): 8529-8543
[6] 张光茹, 王星宇, 吕佳霖, 等. 围栏封育对草地土壤有机碳动态的影响机制研究进展. 生态学报, 2025, 45(16): 8243-8252
[7] Yang PN, Li XL, Li CY, et al. Effects of long-term exclosure on main plant functional groups and their biochemical properties in a patchily degraded alpine meadow in the source zone of the Yellow River, West China. Agronomy, 2023, 13: 2781
[8] Li XH, Li CY, Li XL, et al. Long-term effects of gra-zing exclosure and plateau pika disturbance on soil biochemistry in alpine meadows. Environmental Monitoring and Assessment, 2025, 197: 542
[9] 古琛, 贾志清, 杜波波, 等. 中国退化草地生态修复措施综述与展望. 生态环境学报, 2022, 31(7): 1465-1475
[10] Yang X, Wang BR, Fakher A, et al. Contribution of roots to soil organic carbon: From growth to decomposition experiment. Catena, 2023, 231: 107317
[11] Zhou ZH, Ren CJ, Wang CK, et al. Global turnover of soil mineral-associated and particulate organic carbon. Nature Communications, 2024, 15: 5329
[12] Zheng HJ, Zhang S, Wang GC, et al. Depth-dependent accumulation and controls of particulate and mineral-associated organic carbon in Inner Mongolian grasslands. Science China Earth Sciences, 2025, 68: 3064-3076
[13] Chen L, Zhao XX, Tian QX, et al. Differential effects of fine root- and Mycelium-derived carbon on soil organic carbon in response to warming in an alpine meadow. Geoderma, 2025, 464: 117598
[14] Rocci KS, Lavallee JM, Stewart CE, et al. Soil organic carbon response to global environmental change depends on its distribution between mineral-associated and particu-late organic matter: A meta-analysis. Science of the Total Environment, 2021, 793: 148569
[15] Agnihotri R, Sharma MP, Prakash A, et al. Glycoproteins of arbuscular mycorrhiza for soil carbon sequestration: Review of mechanisms and controls. Science of the Total Environment, 2022, 806: 150571
[16] Sun XY, Xing YJ, Yan GY, et al. Dynamics of glomalin-related soil protein and soil aggregates during secon-dary succession in the temperate forest. Catena, 2024, 234: 107602
[17] Lan X, Zhu ZH, Hou XY, et al. Vegetation restoration promotes glomalin-related soil protein accumulation via mineral interactions in a Karst Region, southwest China. Applied Soil Ecology, 2025, 214: 106333
[18] Yu HY, He WX, Zou YN, et al. Arbuscular mycorrhizal fungi and rhizobia accelerate plant growth and N accumulation and contribution to soil total N in white clover by difficultly extractable glomalin-related soil protein. Applied Soil Ecology, 2024, 197: 105348
[19] Yang X, Wang BR, An SS. Root derived C rather than root biomass contributes to the soil organic carbon sequestration in grassland soils with different fencing years. Plant and Soil, 2021, 469: 161-172
[20] 王占义, 金净, 王成杰, 等. 一种测定荒漠草原根系生物量的内生长土芯法. 土壤, 2017, 49(2): 414-416
[21] Menezes AGS, Filgueiras BKC, Silva CSG, et al. Natural and human-related drivers affect belowground biomass and nutrients in a Caatinga dry forest. Journal of Arid Environments, 2024, 223: 105180
[22] Meng TL, Yue L, Zhou J, et al. Differential responses of plant-soil-microbe C:N:P stoichiometry to nitrogen addition in grassland ecosystems. Catena, 2026, 266: 109920
[23] Zhao YM, Suolunga B, Wang RZ, et al. Effectiveness of long-term enclosure on restoration of Leymus chinensis grassland in Inner Mongolia: Focus on soil carbon storage and fractions. Soil Use and Management, 2026, 42: e70197
[24] Gu R, Xiao KC, Zhu ZH, et al. Afforestation enhances glomalin-related soil protein content but decreases its contribution to soil organic carbon in a subtropical karst area. Journal of Environmental Management, 2024, 356: 120754
[25] 赵晓单, 曾全超, 安韶山, 等. 黄土高原不同封育年限草地土壤与植物根系的生态化学计量特征. 土壤学报, 2016, 53(6): 1541-1551
[26] Wang W, Li MY, Wen QH, et al. Plant-plant interactions drive the decomposition of soil organic carbon via nutrition competition in dryland. Plant, Cell & Environment, 2025, 48: 4756-4769
[27] 苏纪帅, 赵洁, 井光花, 等. 半干旱草地长期封育进程中针茅植物根系格局变化特征. 生态学报, 2017, 37(19): 6571-6580
[28] Zhang QL, Zhou DW, Hu J. Effects of long-term enclosing on distributions of carbon and nitrogen in semi-arid grassland of Inner Mongolia. Ecological Informatics, 2022, 71: 101762
[29] 赵凌平, 白欣, 谭世图, 等. 不同年限封育对黄土高原典型草原地上植被的影响. 草业科学, 2018, 35(1): 27-35
[30] 谢佳轩, 肖孔操, 杨君, 等. 喀斯特峰丛洼地区牧草种植对球囊霉素相关土壤蛋白含量的影响. 湖南生态科学学报, 2025, 12(3): 8-17
[31] Meng LL, He JD, Zou YN, et al. Mycorrhiza-released glomalin-related soil protein fractions contribute to soil total nitrogen in trifoliate orange. Plant, Soil and Environment, 2020, 66: 183-189
[32] Huang JS, Liu WX, Pan SN, et al. Divergent contributions of living roots to turnover of different soil organic carbon pools and their links to plant traits. Functional Ecology, 2021, 35: 2821-2830
[33] 谭文峰, 许运, 史志华, 等. 胶结物质驱动的土壤团聚体形成过程与稳定机制. 土壤学报, 2023, 60(5): 1297-1308
[34] 高瑞, 牛伊宁, 何仁元, 等. 不同种植年限苜蓿地球囊霉素相关土壤蛋白含量及其影响因素. 草业科学, 2024, 41(3): 700-708
[35] Villarino SH, Pinto P, Jackson RB, et al. Plant rhizodeposition: A key factor for soil organic matter formation in stable fractions. Science Advances, 2021, 7: eabd3176

封育草地根系生长与球囊霉素固碳的年限效应

Temporal effects of grassland enclosure on root growth and glomalin-mediated carbon sequestration

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