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Chinese Journal of Applied Ecology ›› 2018, Vol. 29 ›› Issue (2): 678-686.doi: 10.13287/j.1001-9332.201802.028

• Reviews • Previous Articles    

Application of non-invasive microelectrode ion flux estimation technique in crop stress physiology.

LIU Ke1,2, ZHANG Bing-lin2, ZHANG Wen-ying2, ZHANG Yun-bo1,2,3, TIAN Xiao-hai1,2,3*   

  1. 1Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou 434025, Hubei, China;
    2College of Agriculture, Yangtze University, Jingzhou 434025, Hubei, China;
    3Engineering Research Center of Ecology and Agriculture Use of Wetland, Ministry of Education, Jingzhou 434025, Hubei, China
  • Received:2017-04-23 Online:2018-02-18 Published:2018-02-18
  • Contact: E-mail: xiaohait@sina.com
  • Supported by:

    This work was supported by the National Key Research and Development Program of China (2016YFD0300108).

Abstract: Non-invasive microsensing technique has been widely used in evaluating the adaptive responses of plant cells and tissues to abiotic stresses. One of the representative techniques is the microelectrode ion flux estimation (MIFE), which allows concurrent quantification of net fluxes with high spatial and temporal resolution. More importantly, this technique permits simultaneous recording of ion concentration and mobility with less intervention to the in situ physiological status. With the availability of such advanced technique, the last three decades have seen a significant progress towards the role of ion signaling in a variety of abiotic stresses including salinity, extreme temperature, osmotic stress, hypoxia, and drought. In this review, we gave a brief introduction of the MIFE working principles and focused on its applications in detecting ion responses to various abiotic stresses.

Key words: ion flux, crop, abiotic stress., microelectrode ion flux estimation