Effects of drought stress on the stoichiometric characteristics in different organs of three shrub species

doi:10.13287/j.1001-9332.202101.002

Abstract

Abstract: In order to explore how water stress affects the stoichiometric characteristics in leaves, stems, very fine roots (0-1 mm), fine roots (1-2 mm) and thick roots (>2 mm) of three shrub species, we studied the effects of three water treatments [(75±5)%, (55±5)% and (35±5)% of field water capacity (FC)] on the stoichiometric characteristics of different organs of Syringa oblata, Rosa xanthina and Forsythia suspensa in a pot experiment. The results showed that there were significant differences in nitrogen (N) and phosphorus (P) contents, C:N, C:P and N:P of the same organ among the three species. With the intensification of drought stress, there was no significant change of C content in all organs of the three species. The N content increased in leaves, but decreased gradually in stems. The N content in very fine roots and fine roots increased first and then decreased. The P content decreased in leaves and stems, while increased first and then decreased in very fine roots and fine roots. Under drought stress, leaf C:N decreased, C:P and N:P of leaf and stem increased. There was the strongest effect of drought stress on the C:N of very fine roots and C:P and N:P of leaves. There was the least effect of drought stress on C:N, N:P of thick roots and C:P of very fine roots. There was no significant correlation between the contents of C, N in soil and the contents of C, N and P in shrub organs, but soil P content was significantly correlated with the contents of C, N and P in leaves and roots. It was concluded that the relative P limitation in soil was the most important factor affecting the stoichiometric characteristics of shrub organs. Drought had different effects on the stoichiometry of different organs in different shrub species. The stoichio-metry of leaves and very fine roots was more sensitive to drought stress than that of other organs. Drought might affect the stoichiometric characteristics especially related to P in different organs of shrubs, mainly by affecting plant absorption of soil P and its distribution in different organs.

Key words: drought stress, organ, stoichiometric characteristics

CHEN Jia-rui, WANG Guo-liang, MENG Min, WANG Run-chao. Effects of drought stress on the stoichiometric characteristics in different organs of three shrub species[J]. Chinese Journal of Applied Ecology, 2021, 32(1): 73-81.

References

[1] 杨惠敏, 王冬梅. 草-环境系统植物碳氮磷生态化学计量学及其对环境因子的响应研究进展. 草业学报, 2011, 20(2): 244-252 [Yang H-M, Wang D-M. Advances in the study on ecological stoichiometry in grass-environment system and its response to environmental factors. Journal of Prataculture, 2011, 20(2): 244-252]
[2] Gallardo A, Covelo F. Spatial pattern and scale of leaf N and P concentration in a Quercus robur population. Plant & Soil, 2005, 273: 269-277
[3] 银晓瑞, 梁存柱, 王立新, 等. 内蒙古典型草原不同恢复演替阶段植物养分化学计量学. 植物生态学报, 2010, 34(1): 39-47 [Yin X-R, Liang C-Z, Wang L-X, et al. Ecological stoichiometry of plant nutrients at different restoration succession stages in typical steppe of Inner Mongolia, China. Chinese Journal of Plant Ecology, 2010, 34(1): 39-47]
[4] Tessier JT, Rayna DJ. Use of nitrogen to phosphorus ratios in plant tissue as an indicator of nutrient limitation and nitrogen saturation. Journal of Applied Ecology, 2003, 40: 523-534
[5] Hobbie SE, Gough L. Foliar and soil nutrients in tundra on glacial landscapes of contrasting ages in northern Alaska. Oecologia, 2002, 131: 453-462
[6] Reich PB, Oleksyn J, Tilman GD. Global patterns of plant leaf N and P in relation to temperature and latitude. Proceedings of the National Academy of Sciences of the United States of America, 2004, 101: 11001-11006
[7] 陈美玲, 崔君滕, 邓蕾, 等. 黄土高原两种针叶树种不同器官水碳氮磷分配格局及其生态化学计量特征. 地球环境学报, 2018, 9(1): 54-63 [Chen M-L, Cui J-T, Deng L, et al. Distribution patterns and ecological stoichiometry of water, carbon, nitrogen and phosphorus in different organs of two conifer species on the Loess Plateau. Journal of Earth Environment, 2018, 9(1): 54-63]
[8] 陈婵, 王光军, 赵月, 等. 会同杉木器官间C、N、P化学计量比的季节动态与异速生长关系. 生态学报, 2016, 36(23): 7614-7623 [Chen C, Wang G-J, Zhao Y, et al. Seasonal dynamics and allometric growth relationships of C, N, and P stoichiometry in the organs of Cunninghamia lanceolata from Huitong. Acta Ecologica Sinica, 2016, 36(23): 7614-7623]
[9] 刘珮, 马慧, 智颖飙, 等. 9种典型荒漠植物生态化学计量学特征分析. 干旱区研究, 2018, 35(1): 210-219 [Liu P, Ma H, Zhi Y-B, et al. Ecological stoichiometric differences of nine typical eremophyte species. Arid Zone Research, 2018, 35(1): 210-219]
[10] 王娜, 程瑞梅, 肖文发, 等. 三峡库区马尾松根和叶片的生态化学计量特征. 林业科学研究, 2016, 29(4): 536-544 [Wang N, Cheng R-M, Xiao W-F, et al. Ecological stoichiometric characteristics of root and leaf of Pinus massoniana in the three gorges reservoir area. Forest Research, 2016, 29(4): 536-544]
[11] 王凯, 雷虹, 王宗琰, 等. 干旱胁迫下小叶锦鸡儿幼苗C、N、P分配规律及化学计量特征. 林业科学研究, 2019, 32(4): 47-56 [Wang K, Lei H, Wang Z-Y, et al. C, N and P distribution and stoichiometry characteri-stics of Caragana microphylla seedlings to drought stress. Forest Research, 2019, 32(4): 47-56]
[12] 陆媛. 干旱胁迫对刺槐幼苗碳氮磷化学计量特征的影响. 硕士论文. 杨凌: 西北农林科技大学, 2015 [Lu Y. Effects of Drought Stress on Carbon, Nitrogen and Phosphorus Stoichiometry of Robinia pseudoacacia Seedlings. Master Thesis. Yangling: Northwest A＆F University, 2015]
[13] 任书杰, 于贵瑞, 陶波, 等. 兴安落叶松(Larix gmelinii Rupr.)叶片养分的空间分布格局. 生态学报, 2009, 29(4): 1899-1906 [Ren S-J, Yu G-R, Tao B, et al. Spatial patterns for variations in leaf nutrient contents of Dahurian larch. Acta Ecologica Sinica, 2009, 29(4): 1899-1906]
[14] 王凯, 沈潮, 孙冰, 等. 干旱胁迫对科尔沁沙地榆树幼苗C、N、P化学计量特征的影响. 应用生态学报, 2018, 29(7): 2286-2294 [Wang K, Shen C, Sun B, et al. Effects of drought stress on C, N and P stoichiome-try of Ulmus pumila seedlings in Horqin sandy land. Chinese Journal of Applied Ecology, 2018, 29(7): 2286-2294]
[15] 赵子文, 王国梁, 吴阳, 等. 黄土高原不同植被带人工刺槐林土壤氮磷转化酶动力学参数及其温度敏感性. 应用生态学报, 2020, 31(8): 2515-2522 [Zhao Z-W, Wang G-L, Wu Y, et al. Kinetic parameters and temperature sensitivity of soil nitrogen and phosphorus transforming enzymes in Robinia pseudoacacia plantations under different vegetation zones on the Loess Pla-teau, China. Chinese Journal of Applied Ecology, 2020, 31(8): 2515-2522]
[16] Hoch G, Popp M, Korer C. Altitudinal increase of mobile carbon pools in Pinus cembra suggests sink limitation of growth at the Swiss treeline. Oikos, 2002, 98: 361-374
[17] 黄石竹. 水曲柳和落叶松细根养分内循环的研究. 硕士论文. 哈尔滨: 东北林业大学, 2006 [Huang S-Z. The Internal Cycling of Nutrients in Fine Roots of Fraxinus mandshuricea and Larix gemeilinii. Master Thesis. Haerbin: Northeast Forestry University, 2006 ]
[18] Yuan ZY, Chen HYH, Reich PB. Global-scale latitudinal patterns of plant fine-root nitrogen and phosphorus. Nature Communications, 2011, 2: 344
[19] 常文静, 郭大立. 中国温带、亚热带和热带森林45个常见树种细根直径变异. 植物生态学报, 2008, 32(6): 1248-1257 [Chang W-J, Guo D-L. Variation in root diameter among 45 common tree species in tempe-rate, subtropical and tropical forests in China. Chinese Journal of Plant Ecology, 2008, 32(6): 1248-1257]
[20] 李丽, 胡君, 于倩楠, 等. 横断山区高山栎组灌木型植物C、N、P生态化学计量特征. 山地学报, 2018, 36(6): 878-888 [Li L, Hu J, Yu Q-N, et al. Stoichiometric characteristics of carbon, nitrogen and phosphorus in Quercus sect. Heterobalanus shrubs in the Hengduan Mountain. Journal of Mountain Science, 2018, 36(6): 878-888]
[21] Han WX, Fang JY, Guo DL, et al. Leaf nitrogen and phosphrus stoichiometry across 753 terrestrial plant species in China. New Phytologist, 2005, 168: 377-385
[22] Bista DR, Heckathorn SA, Jayawardena DM. Effects of drought on nutrient uptake and the levels of nutrient-uptake proteins in roots of drought-sensitive and -tolerant grasses. Plants, 2018, 7: 28
[23] 王凯, 李依杭, 姜涛, 等. 干旱胁迫对杨树幼苗氮磷化学计量特征及分配格局的影响. 生态学杂志, 2017, 36(11): 124-130 [Wang K, Li Y-H, Jiang T, et al. Effects of drought stress on N and P stoichiometry and allocation of poplar seedlings. Chinese Journal of Ecology, 2017, 36(11): 124-130]
[24] 张香凝, 崔令军, 王保平, 等. 土壤干旱胁迫对Larrea tridentata叶片矿质营养元素含量的影响. 生态环境学报, 2008, 17(6): 2387-2390 [Zhang X-N, Cui L-J, Wang B-P, et al. Effect of soil drought stress on mineral nutrient contents in Larrea tridentata. Ecology and Environmental Sciences, 2008, 17(6): 2387-2390]
[25] Belnap J. Biological Phosphorus Cycling in Dryland Regions. Berlin: Springer, 2011
[26] 李荣, 党维, 蔡靖, 等. 6个耐旱树种木质部结构与栓塞脆弱性的关系. 2016, 40(3): 255-263 [Li R, Dang W, Cai J, et al. Relationships between xylem structure and embolism vulnerability in six species of drought tolerance trees. Chinese Journal of Plant Ecology, 2016, 40(3): 255-263]
[27] Chapin FS. The mineral nutrition of wild plants. Annual Review of Ecology and Systematics, 1980, 11: 233-260
[28] 唐仕姗, 杨万勤, 熊莉, 等. 川西亚高山三种优势树种不同根序碳氮磷化学计量特征. 应用生态学报, 2015, 26(2): 363-369 [Tang S-S, Yang W-Q, Xiong L, et al. C, N and P stoichiometric characteristics of different root orders for three dominant tree species in subalpine forests of western Sichuan, China. Chinese Journal of Applied Ecology, 2015, 26(2): 363-369]
[29] Wright IJ, Reich PB, Westoby M. Strategy shifts in leaf physiology structure and life span nutrient content between species of high- and low-rainfall and high- and low-nutrient habitats. Functional Ecology, 15: 423-434
[30] Sterner RW, Elser JJ. Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere. Princeton, USA: Princeton University Press, 2002: 439
[31] Aerts R, Chapin FS. The mineral nutrition of wild plants revisited: A re-evaluation of processes and patterns. Advances in Ecological Research, 1999, 30: 1-67
[32] 王晶苑, 王绍强, 李纫兰, 等. 中国四种森林类型主要优势植物的C∶N∶P化学计量学特征. 植物生态学报, 2011, 35(6): 587-595 [Wang J-Y, Wang S-Q, Li R-L, et al. C:N:P stoichiometric characteristics of four forest types’ dominant tree species in China. Chinese Journal of Plant Ecology, 2011, 35(6): 587-595]
[33] 贺合亮, 阳小成, 李丹丹, 等. 青藏高原东部窄叶鲜卑花碳、氮、磷化学计量特征. 植物生态学报, 2017, 41(1): 126-135 [He H-L, Yang X-C, Li D-D, et al. Stoichiometric characteristics of carbon, nitrogen and phosphorus of Sibiraea angustata shrub on the eastern Qinghai-Xizang Plateau. Chinese Journal of Plant Ecology, 2017, 41(1): 126-135]
[34] 郑淑霞, 上官周平. 黄土高原地区植物叶片养分组成的空间分布格局. 自然科学进展, 2006, 16(8): 965-973 [Zheng S-X, Shangguan Z-P. Spatial distribution pattern of nutrient composition of plant leaves in the Loess Plateau. Progress in Natural Science, 2006, 16(8): 965-973]
[35] 俞月凤, 彭晚霞, 宋同清. 喀斯特峰丛洼地不同森林类型植物和土壤C、N、P化学计量特征. 应用生态报, 2014, 25(4): 947-954 [Yu Y-F, Peng W-X, Song T-Q, et al. Stoichiometric characteristics of plant and soil C, N and P in different forest types in depressions between karst hills, southwest China. Chinese Journal of Applied Ecology, 2014, 25(4): 947-954]