Abstract: Soil carbohydrates are the important component of soil organic matter, and play an important role in soil aggregation formation. Their hydrolysis methods involve sulfur acid (H₂SO₄), hydrochloric acid (HCl), and trifluoroacetic acid (TFA) hydrolysis, and their determination methods include colorimetry, gasliquid chromatography (GLC), high performance liquid chromatography (HPLC), and high performance anionexchange chromatography with pulsed amperometric detection (HPAE-PAD). This paper summarized the methods of carbohydrates’ hydrolysis, purification and detection, with focus on the derived methods of GLC, and briefly introduced the indication functions of carbohydrates in soil organic matter turnover.

Key words: Soil water stress, Ecological response, Biomass, Pine broadleaf forest