Effects of two Streptomyces strains on growth and physiological properties of Gerbera jamesonii

doi:10.13287/j.1001-9332.202109.029

Abstract

Abstract: We applied different concentrations of spore suspension of Streptomyces exfoliatus FT05W and S. cyaneus ZEA17I to inoculate Gerbera jamesonii to screen for the most effective application concentration. We aimed to explore the effects of two Streptomyces strains on growth and physiological properties of G. jamesonii, and to provide scientific evidence for the application of Streptomyces in G. jamesonii production. The results showed that different concentrations of S. exfoliatus FT05W and S. cyaneus ZEA17I spore suspension could effectively promote the growth of G. jamesonii. In general, S. exfoliatus FT05W performed better than S. cyaneus ZEA17I. S. exfoliatus FT05W (1×10⁹ CFU·mL^-1) could significantly increase the height and crown width of G. jamesonii respectively by 30.2% and 41.5%. Meanwhile, it increased the length and width of the stem. When treated by S. exfoliatus FT05W (1×10⁹ CFU·mL^-1), the content of chlorophyll in G. jamesonii was significantly increased by 65.2%, root activity was significantly increased by 103.3%, and the superoxide dismutase activity was increased by 84.4%. The malondialdehyde content in G. jamesonii was maintained at a low level when treated with the two Streptomyces strains. In summary, S. exfoliatus FT05W and S. cyaneus ZEA17I could effectively promote the growth and physiological properties of G. jamesonii, which could further contribute to its resistance to stress. Therefore, S. exfoliatus FT05W had the potential as a bio-fertilizer for G. jamesonii to solve cultivation obstacles.

Key words: Gerbera jamesonii, Streptomyces, spore suspension, growth, physiological property

HU Xiao-jing, AO Fei-xiong, SHI Le-juan, PENG Qiang, LI Xin, ZHOU Xiao-hui, CHEN Xiao-yulong. Effects of two Streptomyces strains on growth and physiological properties of Gerbera jamesonii[J]. Chinese Journal of Applied Ecology, 2021, 32(9): 3321-3326.

References

[1] 孙翊, 殷丽青, 张永春, 等. 不同LED光质对非洲菊组培苗生长及生理特性的影响. 分子植物育种, 2017, 15(12): 5140-5147 [Sun Y, Yin L-Q, Zhang Y-C, et al. Effect of different LED light qualities on proliferation and physiological characteristics of Gerber jamesonii plantlets. Molecular Plant Breeding, 2017, 15(12): 5140-5147]
[2] 马海燕, 徐瑾, 郑成淑, 等. 非洲菊连作对土壤理化性状与生物性状的影响. 中国农业科学, 2011, 44(18): 3733-3740 [Ma H-Y, Xu J, Zheng C-S, et al. Effects of continuous cropping system on the soil physical-chemical properties and biological properties of Gerbera jamesonii. Scientia Agricultura Sinica, 2011, 44(18): 3733-3740]
[3] 孙远, 王轲永. 花卉连作障碍及治理. 中国花卉园艺, 2017(6): 41 [Sun Y, Wang K-Y. Obstacles and management of flower continuous cropping. China Flo-wers & Horticulture, 2017(6): 41]
[4] 刘亚苓, 于营, 雷慧霞, 等. 植物病害生防因子的作用机制及应用进展. 中国植保导刊, 2019, 39(3): 23-28 [Liu Y-L, Yu Y, Lei H-X, et al. Research progress on mechanisms of biocontrol factors for plant diseases and its application. China Plant Protection, 2019, 39(3): 23-28]
[5] 朱志炎, 田志宏, 李建雄. 链霉菌的功能及其在农业上的应用. 热带亚热带植物学报, 2019, 27(5): 580-590 [Zhu Z-Y, Tian Z-H, Li J-X. Functions and applications of streptomycetes in agriculture. Journal of Tropical and Subtropical Botany, 2019, 27(5): 580-590]
[6] 陶宗, 王海华, 申权, 等. 链霉菌和芽孢杆菌的生防机理及抗菌活性产物. 农业与技术, 2019, 39(14): 1-5 [Tao Z, Wang H-H, Shen Q, et al. Biocontrol mechanisms and antagonistic secondary metabolites of Streptomyces and Bacillus. Agriculture and Technology, 2019, 39(14): 1-5]
[7] 葛优优, 刘晓瑜, 窦桂铭, 等. 内生链霉菌SSD49的抑菌活性和防病促生效果. 生物技术通报, 2017, 33(6): 121-127 [Ge Y-Y, Liu X-Y, Dou G-M, et al. Antimicrobial activities and efficacy of endophytic Streptomyces sp. SSD49 in plant disease control and plant-growth-promoting. Biotechnology Bulletin, 2017, 33(6): 121-127]
[8] Sardi P, Saracchi M, Quaroni S, et al. Isolation of endophytic Streptomyces strains from surface-sterilized roots. Applied and Environmental Microbiology, 1992, 58: 2691-2693
[9] Chen X, Pizzatti C, Bonaldi M, et al. Biological control of lettuce drop and host plant colonization by rhizospheric and endophytic streptomycetes. Frontiers in Microbiology, 2016, 7, doi: 10.3389/fmicb.2016.00714
[10] Chen X, Bonaldi M, Erlacher A, et al. Biocontrol of two Streptomyces spp. strains against lettuce basal drop caused by Sclerotinia sclerotiorum. Phytopathology, 2016, 106, doi: 10.1094/PHYTO-106-4-S2.6
[11] Bonaldi M, Chen X, Kunova A, et al. Colonization of lettuce rhizosphere and roots by tagged Streptomyces. Frontiers in Microbiology, 2015, 6, doi: 10.3389/fmicb.2015.00025
[12] Kunova A, Bonaldi M, Saracchi M, et al. Selection of Streptomyces against soil borne fungal pathogens by a standardized dual culture assay and evaluation of their effects on seed germination and plant growth. BMC Microbiology, 2016, 16: 272, doi: 10.1186/s12866-016-0886-1
[13] 张志良, 瞿伟菁, 李小方. 植物生理学实验指导. 第4版. 北京: 高等教育出版社, 2009 [Zhang Z-L, Qu W-J, Li X-F. Plant Physiology Experimental Guide. 4th Ed. Beijing: Higher Education Press, 2009]
[14] 职明星, 李秀菊. 脯氨酸测定方法的改进. 河南科技学院学报: 自然科学版, 2005, 33(4): 10-12 [Zhi M-X, Li X-J. Improvement of the method for measuring proline content. Journal of Henan Institute of Science and Technology: Natural Science, 2005, 33(4): 10-12]
[15] 张志民, 李海峰, 王帅兵, 等. 隶属函数分析法在大豆品种综合评价中的应用. 农业科技通讯, 2015(6): 88-90 [Zhang Z-M, Li H-F, Wang S-B, et al. Application of membership function analysis method in comprehensive evaluation of soybean varieties. Bulletin of Agricultural Science and Technology, 2015(6): 88-90]
[16] 覃可, 桑维钧, 陈孝玉龙, 等. 烟草拮抗链霉菌FT05W基因组测序与几丁质酶家族基因鉴定. 中国生物防治学报, 2019, 35(3): 463-473 [Qin K, Sang W-J, Chen X-Y-L, et al. Genome sequencing and identification of chitinase family genes of Streptomyces sp. FT05W, an antagonist of tobacco pathogens. Chinese Journal of Biological Control, 2019, 35(3): 463-473]
[17] 王兰英, 徐林波. 生防链霉菌NH14菌株对黄瓜的促生作用研究. 农业科技通讯, 2008(7): 36-38 [Wang L-Y, Xu L-B. Growth promotion effect of biocontrol Streptomyces NH14 on cucumber. Bulletin of Agricultural Science and Technology, 2008(7): 36-38]
[18] 张涛涛, 张虹, 赵娟, 等. 黄赭色链霉菌11F发酵工艺及其对黄瓜幼苗生长的影响.科学技术与工程, 2020, 20(16): 6391-6396 [Zhang T-T, Zhang H, Zhao J, et al. Effect of Streptomyces silaceus 11F on growth of cucumber seedlings and control effect against fusarium wilt. Science Technology and Engineering, 2020, 20(16): 6391-6396]
[19] 连清贵, 甘良, 马青, 等. 番茄灰霉病菌胁迫下壮观链霉菌SC11对寄主的促生作用及防病机理. 植物病理学报, 2016, 46(3): 401-408 [Lian Q-G, Gan L, Ma Q, et al. The disease prevention and growth-promoting effect and mechanism of Streptomyces spectabilis SC11 to Botrytis cinerea stress. Acta Phytopathologica Sinica, 2016, 46(3): 401-408]
[20] 赵娟, 刘霆, 刘伟成, 等. 番茄灰霉病生防链霉菌筛选及鉴定. 微生物学通报, 2019, 46(10): 2548-2558 [Zhao J, Liu T, Liu W-C, et al. Screening and identification of the biocontrol Streptomyces against tomato Botrytis cinerea. Microbiology China, 2019, 46(10): 2548-2558]
[21] 何斐, 张忠良, 崔鸣, 等. 链霉菌剂对魔芋的促生防病作用. 应用与环境生物学报, 2015, 21(5): 866-871 [He F, Zhang Z-L, Cui M, et al. Growth promotion and disease prevention effects of Streptomyces on Amorphophallus konjac K. Koch ex N. E. Br. Chinese Journal of Applied and Environmental Biology, 2015, 21(5): 866-871]
[22] 孙佳瑞. 链霉菌S506促生和提高番茄耐寒耐盐机制的初步研究. 硕士论文. 石家庄: 河北师范大学, 2012 [Sun J-R. Preliminary Study on Mechanisms of Growth Promoting and Cold-salt Tolerance of Tomato Seedlings by Streptomyces sp. S506. Master Thesis. Shijia-zhuang: Hebei Normal University, 2012]
[23] 郭建军, 张永江, 丁方军, 等. 细黄链霉菌(AMCC 400001)对油菜促生作用研究. 长江蔬菜, 2013(24): 58-61 [Guo J-J, Zhang Y-J, Ding F-J, et al. Growth-promoting effects of Streptomyces microflavus (AMCC 400001) on Brassica napus. Journal of Changjiang Vegetables, 2013(24): 58-61]
[24] 刘丽英, 丁文龙, 曹雅杰, 等. 苹果连作生防细菌B6对平邑甜茶幼苗生物量及连作土壤环境的影响. 应用生态学报, 2018, 29(12): 4165-4171 [Liu L-Y, Ding W-L, Cao Y-J, et al. Effects of antagonistic bacteria B6 against the pathogens of apple replant disease on the biomass of Malus hupehensis Rehd seedlings and soil environment under replanting. Chinese Journal of Applied Ecology, 2018, 29(12): 4165-4171]
[25] 温丹, 王晓, 孙凯宁, 等. 不同形态微生物菌剂对不结球白菜生长和品质的影响. 应用生态学报, 2021, 32(5): 1777-1782 [Wen D, Wang X, Sun K-N, et al. Effects of different forms of microbial agents on the growth and quality of Brassica rapa L. ssp. chinensis Makino (non-heading Chinese cabbage). Chinese Journal of Applied Ecology, 2021, 32(5): 1777-1782]