RAPD study on genetic diversity of Frankia

Abstract

Abstract: Eight Frankia strains from 2 taxonomic inoculation groups were analysed for their genetic diversity with Random Amplified Polymorphic DNA(RAPD) technique. Twenty random primerswere used, and the stable RAPDpatterns could be detected with primer OPW15 and OPW16.The molecular weight of amplification products was between 0.5～4Kb, indicating a great genetic diversity withim Frankia strains. Therefore, with proper primer in RAPDanalysis, different Frankia strains could be allocated to the same inoculation group, based on commonbands.

Key words: Frankia, Random Amplified Polymorphic DNA(RAPD), Diversity, cold resistance, anatomical structure, semilethal low temperature (LT₅₀)

Peng Yuandong, Zhang Zhongze . RAPD study on genetic diversity of Frankia[J]. Chinese Journal of Applied Ecology, 1998, 9(1): 59-63.

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