Dynamic changes of respiration pathway and active oxygen levels in subcultured tobacco callus

Abstract

Abstract: During the growth and senescence of tobacco callus, the total respiration rate showed two peaks on the 11th and 19th day,respectively. The development and operation of alternative pathway increased gradually,reached the maximum between two respiration peaks,and then declined in the following days, but the cytochrome pathway was still as a main electron transport pathway consistently. Analysis on the levels of active oxygen species during the growth and senescence process in tobacco callus suggested that H₂O₂ and O₂^{?^-} were involved in some way in the induction of the development and operation of the alternative pathway,but the increase of production rate of OH might suppress both the development and operation of the alternative pathway, which were enhanced by H₂O₂ and O₂^{?^-} respectively. These results were further proved by the use of active oxygen scavengers. The possible participation of the alternative pathway enhanced by active oxygen in the initial senescence process was discussed.

Key words: Tobacco callus, Senescence, Active oxygen, Cyanide resistant respiration

ZHOU Gongke, KONG Yingzhen, LI Hongyu, Wen Jiangqi, LIANG Houguo. Dynamic changes of respiration pathway and active oxygen levels in subcultured tobacco callus[J]. Chinese Journal of Applied Ecology, 2000, (6): 885-888.

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