CO2浓度倍增影响转Bt水稻非靶标害虫褐飞虱的生长发育及取食行为

doi:10.13287/j.1001-9332.201802.036

应用生态学报 ›› 2018, Vol. 29 ›› Issue (2): 651-658.doi: 10.13287/j.1001-9332.201802.036

CO₂浓度倍增影响转Bt水稻非靶标害虫褐飞虱的生长发育及取食行为

逯永清¹, 代阳¹, 于秀英², 于福兰², 江守林¹, 周宗元¹, 陈法军^1*

¹南京农业大学植物保护学院, 南京 210095;
²山东省济阳县农业局, 山东济阳 251400

收稿日期:2017-07-12 出版日期:2018-02-18 发布日期:2018-02-18
通讯作者: E-mail: fajunchen@njau.edu.cn
作者简介:逯永清, 女, 1991年生, 硕士研究生. 主要从事生态学和全球变化生物学研究. E-mail: 2015102056@njau.edu.cn
基金资助:
本文由国家重点基础发展规划项目(2010CB126200)、国家自然科学基金项目(31272051, 31470454)和江苏省“青蓝工程”项目资助

Double-ambient CO₂ concentration affects the growth, development and sucking behavior of non-target brown plant hopper Nilaparvata lugens fed on transgenic Bt rice.

LU Yong-qing¹, DAI Yang¹, YU Xiu-ying², YU Fu-lan², JIANG Shou-lin¹, ZHOU Zong-yuan¹, CHEN Fa-jun^1*

¹College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China;
²Jiyang Bureau of Agriculture, Jiyang 251400, Shandong, China

Received:2017-07-12 Online:2018-02-18 Published:2018-02-18
Contact: E-mail: fajunchen@njau.edu.cn
Supported by:
This work was supported by the National Basic Research Program of China (2010CB126200), the National Natural Science Foundation of China (31272051, 31470454), and the Qinglan Project of Jiangsu Province of China.

摘要/Abstract

摘要： 近年来,大气CO₂浓度升高等全球气候变化和转Bt作物非靶标害虫抗虫性等问题备受关注.大气CO₂浓度升高直接或间接地影响植食性昆虫,而迄今为止有关大气CO₂浓度升高对刺吸式昆虫(同时也是转Bt作物的非靶标害虫)的影响结论不一,且对其刺吸取食行为的影响研究少有报道.本研究利用智能人工气候箱设置CO₂浓度,研究大气CO₂浓度倍增(800 μL·L^-1)对转Bt水稻的非靶标害虫褐飞虱取食行为及其生长发育和繁殖等的影响.结果表明: 大气CO₂浓度倍增对褐飞虱卵和若虫历期、成虫体质量和寿命,以及4龄和5龄若虫的刺吸取食行为等都具有显著影响,但对其繁殖力影响不显著.与对照CO₂浓度(400 μL·L^-1)相比,倍增CO₂浓度处理下褐飞虱的卵和若虫历期及雌成虫寿命分别显著缩短了4.0%、4.2%和6.6%;长翅型成虫比例显著增加了11.6%;初羽化成虫体质量降低,且雌成虫体质量显著降低了2.2%;此外,倍增CO₂浓度处理下褐飞虱4龄和5龄若虫口针的刺探效率都显著增加;其中,N4b波的持续时间分别显著延长了60.0%和50.1%,频次分别显著增加了230.0%和155.9%.可见,CO₂浓度倍增可通过提高褐飞虱的刺吸取食而促进其生长发育,并缩短其世代历期、提高长翅成虫比例,最终导致大气CO₂浓度升高下转Bt水稻的非靶标害虫褐飞虱发生危害严重,并面临其迁飞扩散为害加重的风险.

关键词: EPG技术, 生长发育, CO₂浓度, 褐飞虱, 刺吸取食行为

Abstract: In recent years, the two issues of climate change including elevated CO₂ etc., and resistance of transgenic Bt crops against non-target insect pests have received widespread attention. Elevated CO₂ can affect the herbivorous insects. To date, there is no consensus about the effect of elevated CO₂ on the suck-feeding insect pests (non-target insect pests of transgenic Bt crops). Its effects on the suck-feeding behavior have rarely been reported. In this study, CO₂ levels were set up in artificial climate chamber to examined the effects of ambient (400 μL·L^-1) and double-ambient (800 μL·L^-1) CO₂ levels on the suck-feeding behavior, growth, development, and reproduction of the non-target insect pest of transgenic Bt rice, brown planthopper, Nilaparvata lugens. The results showed that CO₂ level significantly affected the egg and nymph duration, longevity and body mass of adults, and feeding behavior of the 4th and 5th instar nymphs, while had no effect on the fecundity of N. lugens. The duration of eggs and nymphs, and the longevity of female adults were significantly shortened by 4.0%, 4.2% and 6.6% respectively, the proportion of the macropterous adults was significantly increased by 11.6%, and the body mass of newly hatched female adults was significantly decreased by 2.2% by elevated CO₂. In addition, elevated CO₂ significantly enhanced the stylet puncturing efficiency of the 4th and 5th instar nymphs of N. lugens. The duration ofphloem ingestion of the N4b waveform was significantly prolonged by 60.0% and 50.1%, and the frequency significantly was increased by 230.0% and 155.9% for the 4th and 5th instar nymphs of N. lugens by elevated CO₂, respectively. It was concluded that double-ambient CO₂ could promote the growth and development of N. lugens through enhancing its suck-feeding, shorten the generation life-span and increase the macropertous adults’ proportion of N. lugens. Thus, it could result in the occurrence of non-target rice planthopper N. lugens and make the transgenic Bt crops face with harm risk due to migration and diffusion of N. lugens under elevated CO₂.

Key words: CO₂ concentration, EPG technology., Nilaparvata lugens, suck-feeding behavior, growth and development

逯永清, 代阳, 于秀英, 于福兰, 江守林, 周宗元, 陈法军. CO₂浓度倍增影响转Bt水稻非靶标害虫褐飞虱的生长发育及取食行为[J]. 应用生态学报, 2018, 29(2): 651-658.

LU Yong-qing, DAI Yang, YU Xiu-ying, YU Fu-lan, JIANG Shou-lin, ZHOU Zong-yuan, CHEN Fa-jun. Double-ambient CO₂ concentration affects the growth, development and sucking behavior of non-target brown plant hopper Nilaparvata lugens fed on transgenic Bt rice.[J]. Chinese Journal of Applied Ecology, 2018, 29(2): 651-658.

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CO₂浓度倍增影响转Bt水稻非靶标害虫褐飞虱的生长发育及取食行为

Double-ambient CO₂ concentration affects the growth, development and sucking behavior of non-target brown plant hopper Nilaparvata lugens fed on transgenic Bt rice.

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