水稻分蘖期和孕穗期根际反硝化菌群落结构及功能变化

doi:10.13287/j.1001-9332.201904.022

应用生态学报 ›› 2019, Vol. 30 ›› Issue (4): 1344-1350.doi: 10.13287/j.1001-9332.201904.022

水稻分蘖期和孕穗期根际反硝化菌群落结构及功能变化

吴讷^1,2, 邵嘉薇^1,3, 盛荣^1*, 汤亚芳⁴, 张文钊¹, 魏文学¹

¹中国科学院亚热带农业生态研究所亚热带农业生态过程重点实验室, 中国科学院桃源农业生态试验站, 长沙 410125;
²中国科学院大学, 北京 100049;
³华中农业大学资源与环境学院, 武汉 430070;
⁴湖北工程学院生命科学与技术学院, 湖北孝感432000

收稿日期:2018-12-09 出版日期:2019-04-20 发布日期:2019-04-20
通讯作者: * E-mail: shengrong@isa.ac.cn
作者简介:吴讷,女,1995年生,硕士研究生. 主要从事微生物生态研究. E-mail: 931675005@qq.com
基金资助:
本文由国家自然科学基金项目(41501277,41330856)和中国科学院战略性先导科技专项(XDB15020200)资助

Variation of community structure and function of rhizospheric denitrifiers at tillering and booting stages of rice

WU Ne^1,2, SHAO Jia-wei^1,3, SHENG Rong^1*, TANG Ya-fang⁴, ZHANG Wen-zhao¹, WEI Wen-xue¹

¹Key Laboratory of Agro-ecological Processes in Subtropical Regions/Taoyuan Agro-ecosystem Research Station, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha 410125, China;
²University of Chinese Academy of Sciences, Beijing 100049, China;
³College of Resources and Environmental Sciences, Huazhong Agricultural University, Wuhan 430070, China;
⁴College of Life Science and Technology, Hubei Engineering University, Xiaogan 432000, Hubei, China

Received:2018-12-09 Online:2019-04-20 Published:2019-04-20
Contact: * E-mail: shengrong@isa.ac.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (41501277, 41330856) and Strategic Projects of the Chinese Academy of Sciences (XDB15020200).

摘要/Abstract

摘要： 通过水稻盆栽试验,分别于水稻分蘖期和孕穗期采集根际与非根际土壤,利用末端限制性片段长度多态技术(T-RFLP)和实时荧光定量PCR(qPCR)技术探究水稻生长对根际反硝化作用和反硝化微生物的影响.结果表明: 分蘖期根际土壤的反硝化势显著低于非根际土壤,而孕穗期根际与非根际土壤的反硝化势没有显著性差异.但分蘖期和孕穗期根际土壤中含narG和nosZ基因的微生物数量均显著高于非根际土壤,其中含nosZ基因的反硝化微生物的群落组成结构和多样性对根际环境响应更敏感.说明虽然水稻根系生长会刺激反硝化微生物的生长繁殖,但抑制了根际土壤中一些反硝化微生物的活性,从而降低了根际土壤的反硝化潜势.

关键词: 群落组成, 反硝化作用, narG, 根际土壤, nosZ, 反硝化势

Abstract: We investigated the variation of denitrifying communities in rice rhizosphere at tillering and booting stages in comparison with bulk soils with a pot experiment. The techniques of quantitative polymerase chain reaction (qPCR) and terminal restriction fragment length polymorphism (T-RFLP) were used to measure the abundance and community composition of denitrifiers (narG and nosZ), respectively. The results showed that the potential denitrification activity in the rhizosphere at tillering stage was significantly lower than bulk soils. No significant difference was detected between the rhizosphere and bulk soils at booting stage. The abundance of both narG- and nosZ-containing denitrifying bacteria was significantly higher in rhizosphere than in bulk soils at both tillering and booting stages. In comparison with narG-containing community, community composition and diversity of nosZ-containing bacteria were more sensitive to rice growth. In conclusion, the exudates of rice could induce significantly more denitrifying bacteria in rhizosphere, whose denitrifying activities were related to growth stage of rice. At the period with strong growth, the secretion of roots showed clear restriction to the functions of rhizospheric denitrifiers compared to booting stage.

Key words: nosZ, potential denitrification activity, rhizosphere, denitrification, community composition, narG

吴讷, 邵嘉薇, 盛荣, 汤亚芳, 张文钊, 魏文学. 水稻分蘖期和孕穗期根际反硝化菌群落结构及功能变化[J]. 应用生态学报, 2019, 30(4): 1344-1350.

WU Ne, SHAO Jia-wei, SHENG Rong, TANG Ya-fang, ZHANG Wen-zhao, WEI Wen-xue. Variation of community structure and function of rhizospheric denitrifiers at tillering and booting stages of rice[J]. Chinese Journal of Applied Ecology, 2019, 30(4): 1344-1350.

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水稻分蘖期和孕穗期根际反硝化菌群落结构及功能变化

Variation of community structure and function of rhizospheric denitrifiers at tillering and booting stages of rice

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