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应用生态学报 ›› 2023, Vol. 34 ›› Issue (2): 463-470.doi: 10.13287/j.1001-9332.202302.021

• 研究论文 • 上一篇    下一篇

水分胁迫下冬小麦脯氨酸含量高光谱监测

谢永凯1,2, 宋晋瑶1,2, 刘敏1, 孟万忠1, 冯美臣2, 王超2, 杨武德2*, 乔星星2, 杨晨波2   

  1. 1太原师范学院地理科学学院, 山西晋中 030619;
    2山西农业大学农学院, 山西晋中030801
  • 收稿日期:2022-03-28 接受日期:2022-11-16 出版日期:2023-02-15 发布日期:2023-08-15
  • 通讯作者: *E-mail: sxauywd@126.com
  • 作者简介:谢永凯, 男, 1992年生, 博士研究生。主要从事作物生态与信息技术、地理农业等研究。E-mail: xyk720@126.com
  • 基金资助:
    山西省基础研究计划项目(202203021212188)、山西省高等学校科技创新项目(2021L444)和国家自然科学基金项目(31871571)

Hyperspectral monitoring on proline content in winter wheat under water stress

XIE Yongkai1,2, SONG Jinyao1,2, LIU Min1, MENG Wanzhong1, FENG Meichen2, WANG Chao2, YANG Wude2*, QIAO Xingxing2, YANG Chenbo2   

  1. 1Institute of Geography Science, Taiyuan Normal University, Jinzhong 030619, Shanxi, China;
    2College of Agriculture, Shanxi Agricultural University, Jinzhong 030801, Shanxi, China
  • Received:2022-03-28 Accepted:2022-11-16 Online:2023-02-15 Published:2023-08-15

摘要: 干旱灾害频发会严重影响冬小麦的生长发育。通过干旱灾害的模拟,进行不同水分胁迫处理(田间持水量的80%、60%、45%、35%、30%),测定冬小麦游离脯氨酸含量(Pro),研究水分胁迫下冬小麦Pro含量对冠层光谱反射率的响应,通过相关分析法和逐步多元线性回归(CA+SMLR)、偏最小二乘法和逐步多元线性回归(PLS+SMLR)、连续投影算法(SPA)对Pro高光谱特征区域及波段进行提取,使用偏最小二乘回归(PLSR)和多元线性回归(MLR)方法建立Pro预测模型。结果表明: 水分胁迫下,冬小麦Pro含量出现了一定积累,冠层光谱反射率在不同波段范围内发生了规律性变化,说明冬小麦Pro含量对水分胁迫响应敏感。相关分析发现,Pro含量与冠层光谱反射率红边区域的相关性较高,且754、756和761 nm波段对Pro含量变化敏感。构建的PLSR模型表现较好,MLR模型次之,但均有着较好的预测能力和较高的预测精度,说明利用高光谱技术对冬小麦Pro含量进行快速无损监测是可行的。

关键词: 光谱反射率, 冬小麦, 脯氨酸含量, 水分胁迫, 模型

Abstract: Frequent occurrence of drought disaster will seriously affect the growth and development of winter wheat (Triticum aestivum). We set different water stress treatments (80%, 60%, 45%, 35%, 30% of field water capacity) to simulate the severity of drought disaster. We measured free proline content (Pro) of winter wheat, and investigated the responses of Pro to canopy spectral reflectance under water stress. Three methods, i.e., correlation analysis and stepwise multiple linear regression (CA+SMLR), partial least squares and stepwise multiple linear regression (PLS+SMLR), and successive projections algorithm (SPA) were used to extract the hyperspectral cha-racteristic region and characteristic band of proline. Furthermore, partial least square regression (PLSR) and multiple linear regression (MLR) methods were used to establish the predicted models. The results showed that Pro content of winter wheat was higher under water stress, and that the spectral reflectance of canopy changed regularly in different bands, indicating that Pro content of winter wheat was sensitive to water stress. The content of Pro was highly correlated with the red edge of canopy spectral reflectance, with the 754, 756 and 761 nm bands being sensitive to Pro change. The PLSR model performed good, followed by the MLR model, both showing good predictive ability and high model accuracy. In general, it was feasible to monitor Pro content of winter wheat by hyperspectral technique.

Key words: spectral reflectance, winter wheat, proline content, water stress, model