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应用生态学报 ›› 1998, Vol. 9 ›› Issue (3): 323-326.

• 研究论文 • 上一篇    下一篇

Hg对油菜叶细胞膜的损伤及细胞的自身保护作用

马成仓   

  1. 淮北煤炭师范学院生物系, 淮北235000
  • 收稿日期:1996-03-19 修回日期:1997-03-10 出版日期:1998-05-25 发布日期:1998-05-25
  • 基金资助:

    煤炭部煤炭高校优秀青年基金资助项目(95-125);安徽省高校自然科学研究项目(95jl0097)

Hg harm on cell membrane of rape leaf and cell endogenous protection effect

Ma Chengcang   

  1. Huaibei Coal Normal College, Huaibei 235000
  • Received:1996-03-19 Revised:1997-03-10 Online:1998-05-25 Published:1998-05-25

摘要: 砂基培养50d的油菜幼苗,用不同浓度的HgCl2溶液灌溉后,研究叶细胞膜结构与功能及细胞保护系统的变化。结果表明,0.5mg·L-1Hg污灌叶细胞膜结构与功能未见明显变化,细胞保护系统亦无变化;浓度大于1mg·L-1时,叶组织细胞膜脂质过氧化水平升高,膜结构损伤,膜透性增大。1~10mg·L-1Hg污灌,组织蛋白质含量升高,SOD、POD、CAT活性表现出不同程度的升高,细胞呈现出积极性自身保护作用;50mg·L-1Hg污灌,组织蛋白质含量下降,SOD、POD、CAT活性持续下降,细胞自身积极性保护作用消失,说明细胞自身保护系统只能在一定范围内起积极保护作用。

关键词: Hg, 油菜, 叶, 细胞膜, 保护酶

Abstract: In this paper, the harm effect of different HgCl2 concentrations on rape seedlings cultivated in sand for 50 days were studied.The results showed that the structure and function of leaf cell membrane, and the cell endogenous protection system had no measurable changes when the concentration of Hg was 0.5mg·L-1 .But, when the Hg concentration was higher than 1mg · L-1, the membrane lipid peroxidation was quickened, membrane structure was harmed, and the permeability of membrane was increased.At the concentration of 1~10mg Hg·L-1, the soluble protein content of leaf was increased, the activities of SOD, PODand CATwere accelerated in varying degrees, and the cells presented a positive endogenous protection effect.At 50mg Hg·L-1, the soluble protein content was decreased, the activities of SOD, PODand CATwent down steadily, and the positive protection effect disappeared.It is suggested that only within a certain limit, the cell endogenous protection system had a positive protection effect.

Key words: Hg, Rape, Leaf, Cell membrane, Protective enzymes