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应用生态学报 ›› 1999, Vol. 10 ›› Issue (3): 357-361.

• 研究论文 • 上一篇    下一篇

生物学实验中的湿度控制:改良的装置及工作原理

冯明光, 许谦, 徐均焕   

  1. 浙江大学生物科学系, 杭州 310029
  • 收稿日期:1998-06-12 修回日期:1999-02-10 出版日期:1999-05-25 发布日期:1999-05-25
  • 通讯作者: 冯明光,男,41岁,教授,博士,博导.长期从事杀虫微生物学、昆虫病理学、昆虫流行病学、昆虫生态学和害虫微生物防治研究,已发表各种论著逾百篇 E-mail:mgfeng@zjau.edu.cn
  • 基金资助:

    国家杰出青年科学基金(39525004)和国家自然科学基金资助项目(39870513).

Humidity control in biological experiments: modified device and methodology

Feng Mingguang, Xu Qian, Xu Junhuan   

  1. Department of Biological Sciences, Zhejiang University, Hangzhou 310029
  • Received:1998-06-12 Revised:1999-02-10 Online:1999-05-25 Published:1999-05-25

摘要: 相对湿度是生物学实验中一个十分重要而又较难准确控制的环境因素。本项研究设计出一种改进的简易控湿装置,可利用普通温光型培养箱同时进行不同湿度水平的生物学实验。该装置由微型气泵、3个试剂瓶串连而成的三联体和一个用有机玻璃制成的生长箱组成,经在20℃下对饱和酒石酸钠溶液调节的湿度(理论值92%)连续测定和校正,控湿效果良好,误差仅±1%左右。同时对不同浓度硫酸溶液控制生长箱内不同水平的湿度进行了测定和检验,亦效果良好,且对环境温度的适应性强,在5~35℃范围内同一硫酸浓度控制相同的稳定湿度。最后,对上述装置控制湿度的稳定性及其影响因素进行了讨论.

关键词: 湿度控制, 改良装置及工作原理, 生物学实验, 蒸腾耗水量, 圆叶乌桕, 液流密度, 喀斯特, 菜豆树

Abstract: Relative humidity is an environment factor that significantly affects biological experiments, but is difficult to precisely control. Amodified device for easy control of the humidity in regular thermal and light growth chamber was described in this paper. The device included three parts: 1) air pump; 2) three pipe collected containers of salt solution for producing expected humidity; and 3) a perspex chamber, in which, experimental materials can be placed. The modified device worked well for humidity control with an error of only 1%, while a digital data logger was used to monitor the relative humidity controlled by saturated solution of sodium tartrate every three minutes for 6 h at 20℃, which was supposed to yield a 92% r.h. at that temperature. Varying concentrations of sulfate acid were tested as humidity control agents at 5~35℃, with each yielding a stable humidity in the perspex chamber regardless of temperature. The stability of the humidity control system was also discussed when the solutions of sulfate acid were used as humidity control agents.

Key words: Humidity control, Modified device and methodology, Biological experiments, Karst, sap flow density, Triadica rotundifolia, Radermachera sinica, transpiration water consumption.