苏云金芽孢杆菌质粒的检测及功能的初步研究

摘要/Abstract

摘要： 用改进的酶碱综合法检测了苏云金芽孢杆菌4个亚种11个野生菌株的内生质粒,获得良好的制备结果和重现性.对野生菌株YBT-1463及其无质粒突变株BMB171的形态和生理生化特性的比较研究结果表明,YBT-1463的内生质粒携带杀虫晶体蛋白基因,但不携带抗生素的抗性基因,且与该菌株对19种C源和12种N源的利用无关.

关键词: 苏云金芽孢杆菌, 质粒, 检测, 功能

Abstract: The plasmids carried in 11 Bacillus thuringiensis wild-type strains of 4 subspecies were detected using the improved lysozyme-alkaline preparation procedure,and an effective preparation result and recovery was found. By comparing the morphological, physiological and biochemical properties between wild-type strain YBT-1463 and its plasmid-free mutant strain BMB171, it was indicated that the plasmids of YBT-1463 carried insecticidal crystal protein genes,but no resistance genes. There was no relationship between resident plasmids and utilising of 19 sources of carbon and 12 sources of nitrogen in the wild-type strain YBT-1463.

Key words: Bacillus thuringiensis, Plasmid, Detection, Function

中图分类号:

Q343.4

李林, 王征, 喻子牛. 苏云金芽孢杆菌质粒的检测及功能的初步研究[J]. 应用生态学报, 2001, (6): 879-882.

LI Lin, WANG Zheng, YU Ziniu . Detection of Bacillus thuringiensis plasmids and preliminary studiy on their functions[J]. Chinese Journal of Applied Ecology, 2001, (6): 879-882.

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