氯苯胁迫对大豆种子萌发的伤害

应用生态学报 ›› 2002, Vol. ›› Issue (2): 141-144.

氯苯胁迫对大豆种子萌发的伤害

刘宛¹, 孙铁珩¹, 周启星¹, 李培军¹, 许华夏¹, 杨桂芬¹, 张海荣¹, 齐鹏²

1. 中国科学院沈阳应用生态研究所陆地生态过程开放研究实验室, 沈阳 110015;
2. 辽宁大学生物系, 沈阳 110036

收稿日期:2001-07-19 修回日期:2001-08-02
通讯作者: 刘宛,女,1963年生,博士,主要从事污染生态学研究.E-mail:liuwan63@hotmail.com
基金资助:
中国科学院知识创新工程项目(KZCX2401);国家自然科学基金资助项目(29877028)

Chlorobenze-stressing injury of the germination of soybean seed

LIU Wan¹, SUN Tieheng¹, ZHOU Qixing¹, LI Peijun¹, XU Huaxia¹, YANG Guifen¹, ZHANG Hairong¹, QI Peng ²

1. Open Laboratory of Terrestrial Ecological Process, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016;
2. Biology Department of Liaoning University, Shenyang 110036

Received:2001-07-19 Revised:2001-08-02

摘要/Abstract

摘要： 在实验室人工控制条件下,研究了不同浓度1,2,4-三氯苯胁迫对大豆种子萌发和幼苗生长的影响.结果表明,50～200μg·g^-1的1,2,4三氯苯胁迫对大豆种子的发芽率影响甚小,但延迟大豆种子的出苗速率;300μg·g^-1的1,2,3-三氯苯胁迫使种子停止发芽.在正常条件下,大豆种子播种后第4天其下胚轴超氧化物歧化酶(SOD)活性达到高峰,之后趋于稳定.不同浓度1,2,4-三氯苯处理使大豆下胚轴SOD活性下降,丙二醛(MDA)、蛋白质含量及下胚轴直径增加;幼苗的生长受抑制,干重、鲜重有一定程度的下降;而鲜重/干重的比值变化不大.受害程度随着1,2,4-三氯苯浓度的增加而加重.初生根比下胚轴对1,2,4三氯苯胁迫较为敏感.

关键词: 氯苯胁迫, SOD活性, 种子萌发, 大豆, 伤害, 理想生态系统, 空间解析, 景观格局分析, 徽派村落, 三维景观指数

Abstract: Effects of various doses of 1,2,4-trichlorobenze on seed germination and seedling growth of soybean were studied.The results indicated that 50～200 μg·g^-1 of 1,2,4-trichlorobenze had no effect on the percentage of seed germination,but delayed the rate of seedling growth.The seed germination was stopped by 300μg·g^-1 of 1,2,4-trichlorobenze.The most activity of SODtook place on the 4th day after sowing of soybean seed in the normal condition,then tended to be in a certain level with a little alteration.Under the various dose stress of 1,2,4-trichlorobenze,the activity of SODin soybean hypocotyl was decreased,while MDAand protein contents as well as diameter of hypocotyls increased.Seedlings growth,fresh weight(FW),dry weight(DW)were inhibited,while the ratio of FW/DWdidn't change.Damage-level of wheat seedlings was increased with increasing1,2,4-trichlorobenze concentrations.Roots were more sensitive to 1,2,4-trichlorobenze-stress than hypocotyls.

Key words: 1,2,4-trichlorobenze-stress, SOD activity, Seed germination, Soybean, Injury, Huizhou-Styled Village, 3D landscape indices, spatial analysis, landscape pattern analysis, ideal ecosystem model

中图分类号:

X173

刘宛, 孙铁珩, 周启星, 李培军, 许华夏, 杨桂芬, 张海荣, 齐鹏. 氯苯胁迫对大豆种子萌发的伤害[J]. 应用生态学报, 2002, (2): 141-144.

LIU Wan, SUN Tieheng, ZHOU Qixing, LI Peijun, XU Huaxia, YANG Guifen, ZHANG Hairong, QI Peng . Chlorobenze-stressing injury of the germination of soybean seed[J]. Chinese Journal of Applied Ecology, 2002, (2): 141-144.

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