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野生地椒的斑块多样性及空间结构

权俊萍1,2;彭峰3;何树兰3;郑玉红3;江玉梅3;李乃伟3;张明霞3;夏冰3   

  1. 1南京农业大学园艺学院, 南京 210095; 2石河子大学农学院, 新疆石河子 832000; 3江苏省中国科学院植物研究所, 南京 210014
  • 收稿日期:2008-04-09 修回日期:1900-01-01 出版日期:2009-01-20 发布日期:2009-01-20

Patch diversity and spatial structure of wild Thymus quinquecostatus.

QUAN Jun-ping1,2;PENG Feng3;HE Shu-lan3;ZHENG Yu-hong3;JIANG Yu-mei3;LI Nai-wei3;ZHANG Ming-xia3;XIA Bing3   

  1. 1College of Horticulture, Nanjing Agricultural University, Nanjing 210095,China;2College of Agronomy, Shihezi University, Shihezi 832000, Xinjiang, China; 3Institute of Botany, Jiangsu Province & Chinese Academy of Sciences, Nanjing 210014,China
  • Received:2008-04-09 Revised:1900-01-01 Online:2009-01-20 Published:2009-01-20

摘要: 将ISSR、SRAP分子标记与空间自相关分析技术相结合,对我国野生百里香属植物分布最南端 的怀远3个地椒居群进行了以斑块为单位的遗传多样性、克隆多样性和克隆结构、空间结构的研究.结果表明:地椒野生居群内不同斑块间存在较高的遗传多样性和克隆多样性,多样性条带百分率为75.75%,Nei基因多样性指数为0.2537, Shannon信息多样性指数为0.3811,基因型比率平均为0.61,Simpson指数平均为0.96,Fager均匀性指数平均为0.91.居群间的遗传分化较低,在总的遗传多样性中,90.35%来自居群内斑块间的变异,居群间占9.65%.野生地椒居群间无共有基因型斑块,居群内不同斑块间在一定范围内具有镶嵌性,克隆斑块间的分布范围主要集中在0~25 m.该地区地椒居群除了在部分位点呈现一定相关性外,总体缺乏空间结构.该地区野生地椒种群斑块的建立应为种子入侵所致,其后克隆繁殖对斑块的发展及种群的扩张起到了主要作用.

关键词: 生态系统服务, 西南地区, 权衡, 共赢

Abstract: With the combination of ISSR (inter-simple sequence repeats), SRAP (sequence-r elated amplified polymorphism) and spatial autocorrelation, the genetic diversit y and spatial structure per unit patch of three Huaiyuan populations of Thymus quinquecostatus in southeast China were analyzed. The results showed that there e xisted higher levels of genetic and clonal diversity among the patches within th e wild T. quinquecostatus populations, with the percentage of polymorphic lo c being 7575%, Nei’s gene diversity being 02537, Shannon’s information ind ex being 03811, percent of genetype (G/N) being 061, Simpson index (D) bein g 096, and Fager index (E) being 091. Analysis of molecular variance (AMOVA) showed that only 965% of genetic variation resided among the populations, whi le 9035% of it resided among the individuals within the populations. No genoty pe patches in common were observed among the three populations. The spatial dist ribution of the same patches showed a concentrated distribution about 0〖KG-*2〗 -〖KG-*7〗25 m, and that of different patches showed an inlaid distribution. Exc ept for some locations that showed par correlations in the Huaiyuan populations of T. quinquecostatus, most locations lacked in spatial structure according to spatial autocorrelation analysis. The possible mechanism causing the establis hment of the patches of T. quinquecostatus populations was due to seed dispe rsing, and the following clonal reproduction played important roles in patch dev elopment and population expanding.

Key words: Southwest China, trade-off, ecosystem service, win-win