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臭氧浓度升高对油松抗氧化系统活性的影响

阮亚男1,2;何兴元2;陈玮2;陈振举2;孙雨2   

  1. 1辽宁大学生命科学院, 沈阳110036; 2中国科学院沈阳应用生态研究所, 沈阳 110016
  • 收稿日期:2008-09-22 修回日期:1900-01-01 出版日期:2009-05-20 发布日期:2009-05-20

Effects of elevated O3 concentration on anti-oxidative enzyme activities in Pinus tabulaeformis.

RUAN Ya-nan1,2;HE Xing-yuan2;CHEN Wei2;CHEN Zhen-ju2; SUN Yu2   

  1. 1College of Life Sciences, Liaoning University, Shenyang 110036, China;2Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China
  • Received:2008-09-22 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20

摘要: 以生长在开顶箱内的油松为试材,对高浓度臭氧(80 nmol·mol-1)条件下油松(Pinus tabulaeformis)针叶中超氧阴离子自由基(O2·)产生速率、过氧化氢(H2O2)含量、超氧化物歧化酶(SOD)、抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶、单脱氢抗坏血酸还原酶、谷胱甘肽还原酶活性与抗坏血酸(ASA)含量进行测定.结果表明:高浓度臭氧使O2·产生速率提高,H2O2 和MDA含量增加.ASA含量与SOD、抗坏血酸过氧化物酶、脱氢抗坏血酸还原酶、单脱氢抗坏血酸还原酶、谷胱甘肽还原酶活性在高浓度臭氧熏蒸的前期升高,随后下降并低于对照.说明生长季前期,油松抗氧化系统对高浓度臭氧存在适应性反应,但不能抵抗长期臭氧胁迫带来的氧化伤害.

关键词: 长白山, 次生演替, 土壤微生物生物量, 土壤酶活性

Abstract: An open-top chamber experiment was conducted to study the effects of high concentration O3 (80 nmol·mol-1) on the superoxide anion radical (O2〖SX(B-*3〗-〖〗·〖SX)〗) generation rate, hydrogen peroxide (H2O2) content, activities of superoxide dismutase (SOD), ascorbate peroxidase (APX), dehydroascorbate reductase (DHAR), monodehydroascorbate reductase (MDHAR) and glutathione reductase (GR), and ascorbic acid content in Pinus tabulaeformis leaves. Under high concentration O3 exposure, the superoxide anion radical generation rate and H2O2 and malondialdehyde (MDA) contents increased, while the ascorbic acid content and the activities of SOD, APX, DHAR, MDHAR, and GR increased in early growth season but decreased then to a level lower than the control, which illustrated that the antioxidant system of P. tabulaeformis did respond in an acclimation way in the early growth season, but could not bear the damage of long-term elevated O3 exposure.

Key words: Changbai Mountains, secondary succession, soil microbial biomass, soil enzyme activity.