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UV-B辐射增强对三种赤潮微藻DNA的伤害效应

蔡恒江1;唐学玺1;张培玉1,2   

  1. 1中国海洋大学海洋生态学研究室,青岛 266003;2曲阜师范大学生命科学学院,曲阜 273165

  • 收稿日期:2004-03-24 修回日期:2004-07-05 出版日期:2005-03-18

Effects of UV-B radiation enhancement on DNA damage of three red-tide microalgae species

CAI Hengjiang1,TANG Xuexi1,ZHANG Peiyu1,2   

  1. 1Marine Ecology Laboratory,Ocean University of China,Qingdao 266003, China;2College of Life Sciences,Qufu Normal University,Qufu 273165,China

  • Received:2004-03-24 Revised:2004-07-05 Online:2005-03-18

摘要: 运用生态毒理学和生物化学方法研究了UV-B辐射增强对赤潮异弯藻、亚历山大藻和中肋骨条藻DNA的伤害作用.结果表明,3种赤潮微藻的生长状况对UV-B辐射增强的敏感性不同;对UV-B辐射增强的敏感性由高到低依次是赤潮异弯藻、亚历山大藻和中肋骨条藻.随着UV-B辐射剂量的增加,3种赤潮微藻的DNA损伤程度提高,而且赤潮异弯藻DNA的损伤程度明显高于亚历山大藻和中肋骨条藻,亚历山大藻DNA的伤害程度又远远高于中肋骨条藻. UV-B辐射处理解除后,损伤DNA可明显恢复.赤潮异湾藻和亚历山大藻恢复培养6 d,损伤DNA可明显恢复(P<0.05);而中肋骨条藻恢复培养3 d,损伤DNA可明显恢复(P<0.05),说明3种赤潮微藻的DNA损伤水平不适合作为指示UV-B辐射增强的生物学指标.

关键词: 湿地, 藻类生态学

Abstract: The ecotoxicological and biochemical study withHeterosigma akashiwo, Alexandrium tamarense and Skeletonema costatum showed that the test three species of red-tide microalgae had different sensitivities to UVB radiation enhancement,and the order from high to low was Heterosigma akashiwo, Alexandrium tamarense and Skeletonema costatum.The DNA damage of algal cells increased with UV-B radiation enhancement,being more notable in Heterosigma akashiwo than in Alexandrium tamarenseand Skeletonema costatum,and in Alexandrium tamarensethan in Skeletonema costatum.The DNA damage of Heterosigma akashiwo and Alexandrium tamarense could be significantly repaired (P<0.05) after 6 days of UV-B radiation removal,and that of Skeletonema costatum could be significantly repaired (P<0.05) after 3 days,which illustrated that the DNA damage of three red-tide microalgae species could not indicate the damaged degree of marine microalgae resulted from UV-B radiation enhancement.

Key words: Wetland, Algae ecology