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应用生态学报 ›› 2011, Vol. 22 ›› Issue (08): 2201-2210.

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植物盐胁迫应答蛋白质组学研究的技术策略

张恒1,2,戴绍军1,2**   

  1. 1东北林业大学盐碱地生物资源环境研究中心, 哈尔滨 150040;2东北油田盐碱植被恢复与重建教育部重点实验室, 哈尔滨 150040
  • 出版日期:2011-08-18 发布日期:2011-08-18

Technical strategies in the research of plant salt-responsive proteomics: A review.

ZHANG Heng1,2, DAI Shao-jun1,2   

  1. 1Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040, China;2Ministry of Education Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field, Harbin 150040, China
  • Online:2011-08-18 Published:2011-08-18

摘要: 土壤盐渍化是限制植物生长和分布的关键因素之一.揭示植物响应盐胁迫的分子机理是借助分子生物学手段提高植物耐盐性的基础,也是当前植物生理与分子生态学研究的热点问题.高通量的蛋白质组学技术体系包括双向电泳技术、蓝色自然胶电泳技术、双向荧光差异凝胶电泳技术、液相色谱技术,以及各种生物质谱技术,已经被广泛应用于植物应答盐胁迫研究,为解析植物耐盐分子机制提供了重要信息.本文综述了应用于植物盐胁迫响应蛋白质组学研究的技术策略.

关键词: 植物, 蛋白质组学, 盐胁迫, 技术策略

Abstract: Soil salinization is one of the key factors limiting plant growth and distribution.To explore plant molecular salt-responsive mechanisms is the basis of enhancing plant salt tolerance in virtue of molecular biological techniques, and also, the hotspot of plant physiology and molecular ecology. High throughput proteomics approaches include two-dimensional electrophoresis (2-DE), blue-native/SDS-PAGE (BN/SDS-PAGE), two-dimensional fluorescence difference gel electrophoresis (2D DIGE), liquid chromatography (LC), and various mass spectrometry (MS) techniques, being widely applied in the research of plant salt-response and supplied important information for understanding plant molecular salt-tolerant mechanisms. In this paper, a review was made on the technical strategies applied in the research of plant salt-responsive proteomics.

Key words: plant, proteomics, salt stress, technical strategy