水稻化感材料控制稗草的基因定位研究

应用生态学报 ›› 2003, Vol. ›› Issue (12): 2258-2260.

水稻化感材料控制稗草的基因定位研究

徐正浩^1,2,3, 何勇¹, 崔绍荣¹, 赵明³, 张旭³, 李迪²

1. 浙江大学生物系统工程及食品科学学院, 杭州 310029;
2. 中国水稻研究所, 杭州 310006;
3. 中国农业大学作物学院, 北京 100094

收稿日期:2003-07-19 修回日期:2003-10-08
通讯作者: 徐正浩,男,1964年生,博士后,主要从事杂草生态控制和植物化感作用研究,发表论文10余篇.Tel:0571-86971227,E-mail:xzh640909@sohu.com.
基金资助:
国家自然科学基金项目(30170620);国家“十五”科技攻关资助项目(20001BA509B07)

Genes mapping on rice allelopathy against barnyardgrass

XU Zhenghao^1,2,3, HE Yong¹, CUI Shaorong¹, ZHAO Ming³, ZHANG Xu³, LI Di²

1. College of Biosystem Engineering and Food Science, Zhejiang University, Hangzhou 310029, China;
2. China National Rice Research Institute, Hangzhou 310006, China;
3. College of Crop Science, China Agricultural University, Beijing 100094, China

Received:2003-07-19 Revised:2003-10-08

摘要/Abstract

摘要： 利用中156/谷梅2号建立的重组自交系(RILs)所构建的包括168个DNA标记,全长为1447.9cM,基本覆盖水稻基因组12条染色体的连锁图,用差时播种共培法的改进方法对134个该群体的株系及其亲本对无芒稗进行了化感作用评价.用无芒稗的植株干重作为表型定位水稻化感控制稗草的基因.用QTL Mapper1.01b软件进行区间作图,检测到1个与化感作用有关的主效应QTL.该QTL位于第7条染色体上,解释了32.30%的表型变化;检测到6对上位QTL,解释了47.83%的无芒稗干重抑制的变化,主效应和上位效应QTL共解释了80.13%的表型变化.

关键词: 水稻, 差时播种共培法, 化感作用, 无芒稗, QTL

Abstract: By using the relay seeding technique with slight modification,a population of 134 recombinant inbred lines (RILs)derived from a cross between indica rice (Oryza sativa L.) cultivar Zhong 156? Gumei 2 and contained 168 DNA markers covering all 12 chromosomes with 1447.9 cMspans was employed to evaluate the rice allelopathic effect on barnyardgrass [Echinochloa crus galli (l.) Beauv.]. The phenotyping values of the biomass of barnyardgrass infested with rice materials were employed to map the genes of allelopathic activity on barnyardgrass. One main effect of QTL on 7 chromosomes was identified,which explained 32.30% of the phenotypic variation. Six pairs of digenic epistatic loci were also detected,which collectively explained 47.83% of the phenotypic variation. The main QTL and epistatic loci totally explained 80.13% of the phenotypic variation of allelopathic activity.

Key words: Rice, Relay seeding technique, Allelopathic effect, Barnyardgrass, Quantitative trait loci (QTL)

中图分类号:

Q948

徐正浩, 何勇, 崔绍荣, 赵明, 张旭, 李迪. 水稻化感材料控制稗草的基因定位研究[J]. 应用生态学报, 2003, (12): 2258-2260.

XU Zhenghao, HE Yong, CUI Shaorong, ZHAO Ming, ZHANG Xu, LI Di. Genes mapping on rice allelopathy against barnyardgrass[J]. Chinese Journal of Applied Ecology, 2003, (12): 2258-2260.

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