日光温室连作土壤酚类物质变化及其对黄瓜根系抗病性相关酶的影响

应用生态学报 ›› 2005, Vol. 16 ›› Issue (1): 79-82.

日光温室连作土壤酚类物质变化及其对黄瓜根系抗病性相关酶的影响

马云华, 魏珉, 王秀峰

山东农业大学园艺学院, 泰安, 271018

收稿日期:2003-11-18 修回日期:2004-03-26 出版日期:2005-01-15 发布日期:2005-01-15
通讯作者: 王秀峰
基金资助:
国家"十五"科技攻关资助项目(2001BA503B04).

Dynamics of soil phenols in continuous cropping solar greenhouse and their effects on disease resistance-related enzyme activities in cucumber root

MA Yunhua, WEI Min, WANG Xiufeng

College of Horticulture, Shandong Agricultural University, Taian 271018, China

Received:2003-11-18 Revised:2004-03-26 Online:2005-01-15 Published:2005-01-15

摘要/Abstract

摘要： 研究了日光温室连作黄瓜土壤酚类物质变化及其对黄瓜根系抗病性相关酶的影响.结果表明,随着连作年限的增加,对羟基苯甲酸、阿魏酸、苯甲酸以及总量呈现明显的积累特征:种植5年、7年、9年的含量显著高于1年、3年,且9年的是1年的2倍.在低浓度处理下(0和80 μg·g^-1),前期(2、3叶期)诱导多酚氧化酶、过氧化物酶、苯丙氨酸转氨酶活性不同程度地升高,植株表现高抗性,在较高浓度下(120和160μg·g^-1),处理前期多酚氧化酶、过氧化物酶、苯丙氨酸转氨酶活性迅速降低,甚至丧失酶活性.连作土壤酚类物质浓度变化是影响日光温室黄瓜抗病性的主要因素之一.

关键词: 日光温室, 连作, 酚类物质, 黄瓜, 抗病性相关酶

Abstract: The study showed that under solar greenhouse condition,the contents of soil p-hydroxybenzoic acid,ferulic acid and benzoic acid and their total amounts increased with increasing continuous cropping years,and were obviously higher after continuously cropped for 5,7 and 9 years than for 1 and 3 years.Different concentration of soil phenols resulted in completely different effects on disease resistance-related enzyme activities in cucumber root,e.g.,under lower concentrations (40 and 80 μg·g^-1),the activities of phenylalanine(PAL),ployphenol oxidase(PPO) and peroxidase(POD) were increased,while under higher levels (120 and 160 μg·g^-1),they were restrained.Therefore,the changes of soil phenol contents in continuous cropping solar greenhouse could be one of the essential factors affecting the disease-resistance of cucumber.

Key words: Solar greenhouse, Continuous cropping, Phenols, Cucumber, Disease-related resistance

中图分类号:

S154.2

马云华, 魏珉, 王秀峰. 日光温室连作土壤酚类物质变化及其对黄瓜根系抗病性相关酶的影响[J]. 应用生态学报, 2005, 16(1): 79-82.

MA Yunhua, WEI Min, WANG Xiufeng . Dynamics of soil phenols in continuous cropping solar greenhouse and their effects on disease resistance-related enzyme activities in cucumber root [J]. Chinese Journal of Applied Ecology, 2005, 16(1): 79-82.

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