Biodiversity of Frankia strains in nodules from Alnus and Hippophae by ARDRA

Abstract

Abstract: S ribosomal DNAs(rDNAs) of total 91 samples of Frankia strains in root nodules from Alnus and Hippophae were ampilified by PCR with primer rD1 and fD1.A fragment about 1500bp long was obtained.The amplicons were digested by HaeⅢ and Afa Ⅰ together and run in agarose gels.As a result,48 Alnus-infected Frankia were distinguished into three different groups and 43 Hippophae-infected Frankia into three different groups,respectively.The results showed that there is great genetic diversity among the Frankia strains directly from root nodules.

Key words: Alnus, Hippophae nodule, Frankia, Diversity, ARDRA

CLC Number:

Q939.13

WU Shaohui, ZHANG Huiwen, XIONG Zhi, LIU Zhong, ZHANG Zhongze, ZHANG Chenggang. Biodiversity of Frankia strains in nodules from Alnus and Hippophae by ARDRA[J]. Chinese Journal of Applied Ecology, 2001, (6): 883-886.

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