天然林转人工林对亚热带森林土壤团聚体中亚硝酸盐还原基因丰度的影响

doi:10.13287/j.1001-9332.202301.005

应用生态学报 ›› 2023, Vol. 34 ›› Issue (1): 25-30.doi: 10.13287/j.1001-9332.202301.005

天然林转人工林对亚热带森林土壤团聚体中亚硝酸盐还原基因丰度的影响

邓米林^1,2, 叶桂萍³, 胥超^2,4, 宛颂^1,2, 贺纪正^1,2, 林永新^1,2*

¹湿润亚热带山地生态国家重点实验室培育基地, 福州 350007;
²福建师范大学地理科学学院, 福州 350007;
³闽江学院海洋研究院, 福州 350108;
⁴福建三明森林生态系统与全球变化国家野外科学观测研究站, 福建三明 365000

收稿日期:2022-08-15 修回日期:2022-10-12 出版日期:2023-01-15 发布日期:2023-06-15
通讯作者: ^*E-mail: yxlin@fjnu.edu.cn
作者简介:邓米林, 男, 1998年生, 硕士研究生。主要从事土壤微生物生态学研究。E-mail: 1550342935@qq.com
基金资助:
国家自然科学基金项目(42077041)和福建省自然科学基金项目(2020J01187)

Effects of conversion of natural forest to plantations on the abundance of nitrite reducing genes in soil aggregates in subtropical forest region

DENG Mi-lin^1,2, YE Gui-ping³, XU Chao^2,4, WAN Song^1,2, HE Ji-zheng^1,2, LIN Yong-xin^1,2*

¹Cultivation Base of State Key Laboratory for Subtropical Mountain Ecology, Fuzhou 350007, China;
²School of Geographical Sciences, Fujian Normal University, Fuzhou 350007, China;
³Institute of Oceanography, Minjiang University, Fuzhou 350108, China;
⁴Fujian Sanming Forest Ecosystem and Global Change National Observation and Research Station, Sanming 365000, Fujian, China

Received:2022-08-15 Revised:2022-10-12 Online:2023-01-15 Published:2023-06-15

摘要/Abstract

摘要： 我国亚热带地区大面积天然林已转变为人工林,对森林生态系统结构和功能产生了极大影响。为揭示森林土壤团聚体中N₂O产生的关键基因亚硝酸盐还原基因(nirK和nirS)对森林转换后的响应特征,本研究选取中亚热带米槠天然林、杉木人工林和马尾松人工林为对象,分析了3种林分土壤和团聚体中nirK和nirS基因丰度。结果表明: 天然林转变成人工林后,土壤pH值升高,但铵态氮含量下降。森林转换对土壤团聚体结构组成影响不大,但不同粒径团聚体中nirK和nirS基因丰度存在差异,以小团聚体分布最多,粉-黏颗粒分布最少。各林分土壤中nirK基因丰度均显著高于nirS基因丰度,表明nirK在酸性森林土壤中占主导。天然林转人工林显著增加全土和团聚体中nirK及nirS基因丰度,表明森林转换有利于提高nirK和nirS基因丰度,这可能与pH值的提高有关。综上,天然林转变为杉木或马尾松人工林显著提高了土壤和团聚体中nirK和nirS丰度,但对团聚体质量分数无显著影响。

关键词: 森林转换, 土壤团聚体, nirK, nirS

Abstract: Large proportion of natural forest has been transformed into plantations in subtropical regions, with consequences on forest ecosystem structure and function. In order to understand the responses of two nitrite reducing genes (nirK and nirS) in N₂O production to forest conversion, we collected soil samples from Castanopsis carlesii natural forest, Cunninghamia lanceolata plantation and Pinus massoniana plantation and examined the abundance of nirK and nirS genes in soils and aggregates. Results showed that forest conversion increased soil pH, while decreased soil ammonium content. Forest conversion did not influence the mass proportion of soil aggregates. The abundance of nirK and nirS genes varied in aggregates with different particle sizes. The abundance of nirK and nirS genes was the highest in small macraoaggregates and the lowest in the silt-clay particles. Moreover, the abundance of nirK was significantly higher than that of nirS in soils of all forest types, indicating that nirK dominated in the acidic forest soils. Conversion of natural forest to plantations significantly increased the abundance of nirK and nirS genes in the bulk soil and aggregates, indicating that forest conversion would be beneficial for the growth of microorganisms bearing nirK and nirS genes, which might be associated with the increases of soil pH. Taken together, conversion of natural forest to C. lanceolata plantation or P. massoniana plantation significantly increased the abundance of nirK and nirS in soils and aggregates, but did not affect the mass proportions of aggregates.

Key words: forest conversion, soil aggregate, nirK, nirS.

邓米林, 叶桂萍, 胥超, 宛颂, 贺纪正, 林永新. 天然林转人工林对亚热带森林土壤团聚体中亚硝酸盐还原基因丰度的影响[J]. 应用生态学报, 2023, 34(1): 25-30.

DENG Mi-lin, YE Gui-ping, XU Chao, WAN Song, HE Ji-zheng, LIN Yong-xin. Effects of conversion of natural forest to plantations on the abundance of nitrite reducing genes in soil aggregates in subtropical forest region[J]. Chinese Journal of Applied Ecology, 2023, 34(1): 25-30.

参考文献

[1] Ravishankara AR, Daniel JS, Portmann RW. Nitrous oxide (N₂O): The dominant ozone-depleting substance emitted in the 21st century. Science, 2009, 326: 123-125
[2] Conrad R. Soil microorganisms as controllers of atmospheric trace gases (H₂, CO, CH₄, OCS, N₂O, and NO). Microbiological Reviews, 1996, 60: 609-640
[3] Kuypers MMM, Marchant HK, Kartal B. The microbial nitrogen-cycling network. Nature Reviews Microbiology, 2018, 16: 263-276
[4] Wei W, Isobe K, Nishizawa T, et al. Higher diversity and abundance of denitrifying microorganisms in environments than considered previously. The ISME Journal, 2015, 9: 1954-1965
[5] Amezketa E. Soil aggregate stability: A review. Journal of Sustainable Agriculture, 1999, 14: 83-151
[6] Sexstone AJ, Parkin TB, Tiedje JM. Denitrification response to soil wetting in aggregated and unaggregated soil. Soil Biology and Biochemistry, 1988, 20: 767-769
[7] Sey BK, Manceur AM, Whalen JK, et al. Small-scale heterogeneity in carbon dioxide, nitrous oxide and methane production from aggregates of a cultivated sandy-loam soil. Soil Biology and Biochemistry, 2008, 40: 2468-2473
[8] Bandyopadhyay KK, Lal R. Effect of land use management on greenhouse gas emissions from water stable aggregates. Geoderma, 2014, 232: 363-372
[9] Khalil K, Renault P, Mary B. Effects of transient anaerobic conditions in the presence of acetylene on subsequent aerobic respiration and N₂O emission by soil aggregates. Soil Biology and Biochemistry, 2005, 37: 1333-1342
[10] Morales SE, Cosart T, Holben WE. Bacterial gene abundances as indicators of greenhouse gas emission in soils. The ISME Journal, 2010, 4: 799-808
[11] Li PP, Zhang SQ, Li F, et al. Long term combined fertilization and soil aggregate size on the denitrification and community of denitrifiers. Applied Soil Ecology, 2020, 156: 103718
[12] Blaud A, van der Zaan B, Menon M, et al. The abundance of nitrogen cycle genes and potential greenhouse gas fluxes depends on land use type and little on soil aggregate size. Applied Soil Ecology, 2018, 125: 1-11
[13] Bouwman AF, van der Hoek KW, Olivier JGJ. Uncertainties in the global source distribution of nitrous oxide. Journal of Geophysical Research: Atmospheres, 1995, 100: 2785-2800
[14] Luo X, Hou E, Chen J, et al. Dynamics of carbon, nitrogen, and phosphorus stocks and stoichiometry resulting from conversion of primary broadleaf forest to plantation and secondary forest in subtropical China. Catena, 2020, 193: 104606
[15] Konda R, Ohta S, Ishizuka S, et al. Spatial structures of N₂O, CO₂, and CH₄ fluxes from Acacia mangium plantation soils during a relatively dry season in Indonesia. Soil Biology and Biochemistry, 2008, 40: 3021-3030
[16] Tang W, Chen D, Phillips OL, et al. Effects of long-term increased N deposition on tropical montane forest soil N₂ and N₂O emissions. Soil Biology and Biochemistry, 2018, 126: 194-203
[17] Elliott ET. Aggregate structure and carbon, nitrogen, and phosphorus in native and cultivated soils. Soil Science Society of America Journal, 1986, 50: 627-633
[18] 鲁如坤. 土壤农业化学分析方法. 北京: 中国农业科技出版社, 2000
[19] 宛颂, 段春健, 樊剑波, 等. 旱地红壤反硝化功能基因丰度对长期施肥的响应. 应用生态学报, 2020, 31(11): 3729-3736
[20] 王小红, 杨智杰, 刘小飞. 等. 天然林转换成人工林对土壤团聚体稳定性及有机碳分布的影响. 水土保持学报, 2014, 28(6): 177-182
[21] 张楠, 杨智杰, 胥超, 等. 中亚热带森林转换对凋落物养分归还及养分利用效率的影响. 应用生态学报, 2022, 33(2): 321-328
[22] 宋庆妮, 杨清培, 余定坤, 等. 赣中亚热带森林转换对土壤氮素矿化及有效性的影响. 生态学报, 2013, 33(22): 7309-7318
[23] 吴鹏, 王襄平, 张新平, 等. 东北地区森林凋落叶分解速率与气候、林型、林分光照的关系. 生态学报, 2016, 36(8): 2223-2232
[24] Breulmann M, Schulz E, Weißhuhn K, et al. Impact of the plant community composition on labile soil organic carbon, soil microbial activity and community structure in semi-natural grassland ecosystems of different productivity. Plant and Soil, 2012, 352: 253-265
[25] Allen K, Corre MD, Tjoa A, et al. Soil nitrogen-cycling responses to conversion of lowland forests to oil palm and rubber plantations in Sumatra, Indonesia. PLoS One, 2015, 10(7): e0133325
[26] 纪娇娇, 郑蔚, 杨智杰, 等. 亚热带森林转换对土壤微生物呼吸及其熵值的影响. 生态学报, 2020, 40(3): 800-807
[27] Hu B, Yang B, Pang X, et al. Responses of soil phosphorus fractions to gap size in a reforested spruce forest. Geoderma, 2016, 279: 61-69
[28] Lehmann A, Zheng W, Rillig MC. Soil biota contributions to soil aggregation. Nature Ecology & Evolution, 2017, 1: 1828-1835
[29] 李娜, 韩晓增, 尤孟阳, 等. 土壤团聚体与微生物相互作用研究. 生态环境学报, 2013, 22(9): 1625-1632
[30] Guggenberger G, Elliott ET, Frey SD, et al. Microbial contributions to the aggregation of a cultivated grassland soil amended with starch. Soil Biology and Biochemistry, 1999, 31: 407-419
[31] 陈山, 杨峰, 林杉, 等. 土地利用方式对红壤团聚体稳定性的影响. 水土保持学报, 2012, 26(5): 211-216
[32] Bárta J, Melichová T, Vaněk D, et al. Effect of pH and dissolved organic matter on the abundance of nirK and nirS denitrifiers in spruce forest soil. Biogeochemistry, 2010, 101: 123-132
[33] Shein EV. Course of Soil Physics. Moscow: Moscow State University Publishing, 2005
[34] 李文娟, 蔡延江, 朱同彬, 等. 土壤团聚体氧化亚氮排放及其微生物学机制研究进展. 土壤学报, 2021, 58(5): 1132-1144
[35] Neumann D, Heuer A, Hemkemeyer M, et al. Response of microbial communities to long-term fertilization depends on their microhabitat. FEMS Microbiology Eco-logy, 2013, 86: 71-84

天然林转人工林对亚热带森林土壤团聚体中亚硝酸盐还原基因丰度的影响

Effects of conversion of natural forest to plantations on the abundance of nitrite reducing genes in soil aggregates in subtropical forest region

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