氮添加诱导的磷限制改变了亚热带黄山松林土壤微生物群落结构

doi:10.13287/j.1001-9332.202301.042

应用生态学报 ›› 2023, Vol. 34 ›› Issue (1): 203-212.doi: 10.13287/j.1001-9332.202301.042

氮添加诱导的磷限制改变了亚热带黄山松林土壤微生物群落结构

张晓晴^1,2, 曾泉鑫^1,2, 元晓春^1,2,3, 万晓华^1,2, 崔琚琰^1,2, 李文周⁴, 林惠瑛^1,2, 谢欢^1,2, 陈文伟⁴, 吴君梅^1,2, 陈岳民^1,2*

¹福建师范大学湿润亚热带山地生态国家重点实验室培育基地, 福州 350007;
²福建师范大学地理科学学院, 福州 350007;
³武夷学院旅游学院, 福建武夷山 354300;
⁴福建戴云山国家级自然保护区管理局, 福建泉州 362500

收稿日期:2022-03-20 修回日期:2022-11-22 出版日期:2023-01-15 发布日期:2023-06-15
通讯作者: ^*E-mail: ymchen@fjnu.edu.cn
作者简介:张晓晴, 女, 1998年生, 硕士研究生。主要从事全球变化背景下森林生态系统磷循环和土壤微生物研究。E-mail: 573110872@qq.com
基金资助:
福建省自然科学基金项目(2020J01142,2020J01397)和安徽省自然科学基金项目(2108085QC105)

Phosphorus limitation induced by nitrogen addition changed soil microbial community structure in a subtropical Pinus taiwanensis forest

ZHANG Xiao-qing^1,2, ZENG Quan-xin^1,2, YUAN Xiao-cun^1,2,3, WAN Xiao-hua^1,2, CUI Ju-yan^1,2, LI Wen-zhou⁴, LIN Hui-ying^1,2, XIE Huan^1,2, CHEN Wen-wei⁴, WU Jun-mei^1,2, CHEN Yueh-min^1,2*

¹Cultivation Base of State Key Laboratory of Humid Subtropical Mountain Ecology, Fujian Normal University, Fuzhou 350007, China;
²School of Geographical Science, Fujian Normal University, Fuzhou 350007, China;
³College of Tourism, Wuyi University, Wuyishan 354300, Fujian, China;
⁴Daiyun Mountain National Nature Reserve Administration Bureau, Quanzhou 362500, Fujian, China

Received:2022-03-20 Revised:2022-11-22 Online:2023-01-15 Published:2023-06-15

摘要/Abstract

摘要： 土壤微生物在陆地生态系统的生物地球化学循环中起着重要作用。然而目前尚不清楚氮(N)添加量及其持续时间如何影响土壤微生物群落结构,以及微生物群落结构变化与微生物相对养分限制状况是否存在关联。本研究在亚热带黄山松林开展了N添加试验以模拟N沉降,并设置3个处理:对照(CK, 0 kg N·hm^-2·a^-1)、低N(LN, 40 kg N·hm^-2·a^-1)和高N(HN, 80 kg N·hm^-2·a^-1)。在N添加满1年和3年时测定土壤基本理化性质、磷脂脂肪酸含量和碳(C)、N、磷(P)获取酶活性,并通过生态酶化学计量分析土壤微生物的相对养分限制状况。结果表明: 1年N添加对土壤微生物群落结构无显著影响,3年LN处理显著提高了革兰氏阳性菌(G⁺)、革兰氏阴性菌(G^-)、放线菌(ACT)和总磷脂脂肪酸(TPLFA)含量,而3年HN处理对微生物的影响不显著,表明细菌和ACT对N添加可能更为敏感。N添加加剧了微生物C和P限制,而P限制是土壤微生物群落结构变化的最佳解释因子。这表明,N添加诱导的P限制可能更有利于部分贫营养菌(如G⁺)和参与P循环的微生物(如ACT)的生长,从而改变亚热带黄山松林土壤微生物群落结构。

关键词: 氮添加, 磷酸脂肪酸, 磷限制, 碳限制

Abstract: Soil microorganisms play an important role in the biogeochemical cycles of terrestrial ecosystems. How-ever, it is still unclear how the amount and duration of nitrogen (N) addition affect soil microbial community structure and whether there is a correlation between the changes in microbial community structure and their nutrient limi-tation status. In this study, we conducted an N addition experiment in a subtropical Pinus taiwanensis forest to simulate N deposition with three treatments: control (CK, 0 kg N·hm^-2·a^-1), low N (LN, 40 kg N·hm^-2·a^-1), and high N (HN, 80 kg N·hm^-2·a^-1). Basic soil physicochemical properties, phospholipid fatty acids content, and carbon (C), N and phosphorus (P) acquisition enzyme activities were measured after one and three years of N addition. The relative nutrient limitation status of soil microorganisms was analyzed using ecological enzyme stoichiometry. The results showed that one-year N addition did not affect soil microbial community structure. Three-year LN treatment significantly increased the contents of Gram-positive bacteria (G⁺), Gram-negative bacteria (G^-), actinomycetes (ACT), and total phospholipid fatty acids (TPLFA), whereas three-year HN treatment did not significantly affect soil microbial community, indicating that bacteria and ACT might be more sensitive to N addition. Nitrogen addition exacerbated soil C and P limitation. Phosphorus limitation was the optimal explanatory factor for the changes in soil microbial community structure. It suggested that P limitation induced by N addition might be more beneficial for the growth of certain oligotrophic bacteria (e.g. G⁺) and the microorganisms participating in the P cycling (e.g. ACT), with consequences on soil microbial community structure of subtropical Pinus taiwanensis forest.

Key words: nitrogen addition, phospholipid fatty acids, phosphorus limitation, carbon limitation.

张晓晴, 曾泉鑫, 元晓春, 万晓华, 崔琚琰, 李文周, 林惠瑛, 谢欢, 陈文伟, 吴君梅, 陈岳民. 氮添加诱导的磷限制改变了亚热带黄山松林土壤微生物群落结构[J]. 应用生态学报, 2023, 34(1): 203-212.

ZHANG Xiao-qing, ZENG Quan-xin, YUAN Xiao-cun, WAN Xiao-hua, CUI Ju-yan, LI Wen-zhou, LIN Hui-ying, XIE Huan, CHEN Wen-wei, WU Jun-mei, CHEN Yueh-min. Phosphorus limitation induced by nitrogen addition changed soil microbial community structure in a subtropical Pinus taiwanensis forest[J]. Chinese Journal of Applied Ecology, 2023, 34(1): 203-212.

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氮添加诱导的磷限制改变了亚热带黄山松林土壤微生物群落结构

Phosphorus limitation induced by nitrogen addition changed soil microbial community structure in a subtropical Pinus taiwanensis forest

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