氮沉降驱动下凋落物分解对毛竹林土壤有机碳组分的影响

doi:10.13287/j.1001-9332.202411.015

应用生态学报 ›› 2024, Vol. 35 ›› Issue (11): 2983-2991.doi: 10.13287/j.1001-9332.202411.015

氮沉降驱动下凋落物分解对毛竹林土壤有机碳组分的影响

姜铭楷^1,2, 马书琴³, 熊艳云⁴, 吴逸卿⁴, 吴淑倩⁴, 钱金瑶^1,2, 陈有超^1,2*, 蔡延江^1,2

¹浙江农林大学省部共建亚热带森林培育国家重点实验室, 杭州 311300;
²浙江农林大学环境与资源学院/碳中和学院, 杭州 311300;
³湖南财政经济学院经济地理学院, 长沙 410205;
⁴钱江源-百山祖国家公园庆元保护中心, 浙江丽水 323800

收稿日期:2024-05-29 修回日期:2024-09-09 出版日期:2024-11-18 发布日期:2025-05-18
通讯作者: *E-mail: chenyouchao@zafu.edu.cn
作者简介:姜铭楷, 男, 1999年生, 硕士研究生。主要从事农业资源与环境研究。E-mail: jiangmingkai2022@163.com
基金资助:
国家自然科学基金项目(32371844)、百山祖园区土壤碳储量空间分布特征项目(2023JBGS04)和浙江省“三农九方”科技协作计划项目(2023SNJF037)

Impact of litter decomposition driven by nitrogen deposition on the soil organic carbon fractions in a Moso bamboo forest

JIANG Mingkai^1,2, MA Shuqin³, XIONG Yanyun⁴, WU Yiqing⁴, WU Shuqian⁴, QIAN Jinyao^1,2, CHEN Youchao^1,2*, CAI Yanjiang^1,2

¹State Key Laboratory of Subtropical Silviculture, Zhejiang A＆F University, Hangzhou 311300, China;
²College of Environment and Resources/College of Carbon Neutrality, Zhejiang A＆F University, Hangzhou 311300, China;
³School of Economic Geography, Hunan University of Finance and Econo-mics, Changsha 410205, China;
⁴Qingyuan Conservation Center, Qianjiangyuan-Baishanzu National Park, Lishui 323800, Zhejiang, China

Received:2024-05-29 Revised:2024-09-09 Online:2024-11-18 Published:2025-05-18

摘要/Abstract

摘要： 土壤有机碳的周转及稳定与氮沉降和凋落物分解密切相关。然而,关于氮沉降驱动下毛竹凋落物分解如何影响土壤不同有机碳组分,还存在较大不确定性。为了探究氮沉降驱动下凋落物分解对土壤有机碳组分的影响,本研究依托浙江农林大学安吉毛竹林氮沉降试验基地,设置氮处理(N,施氮量50 kg N·hm^-2·a^-1;CK,施等量水对照)和凋落物处理(L,凋落物保留;LR,凋落物去除),分析了各处理下凋落物分解的质量损失及土壤理化性质、颗粒态有机碳(POC)、矿物结合态有机碳(MAOC)和土壤胞外酶活性(EEAs)。结果表明: 施氮处理显著降低了毛竹叶凋落物的质量损失。施氮处理可显著增加POC含量,但显著降低了MAOC含量,而凋落物保留可同时显著增加POC和MAOC含量。施氮处理均显著降低了β-葡萄糖苷酶(BG)、β-木糖苷酶(BX)、纤维二糖水解酶(CBH)、N-乙酰-β-D氨基葡萄糖苷酶(NAG)、酚氧化酶(POX)和过氧化物酶(PER)活性,而凋落物保留处理显著增加了BG、POX和PER活性。相关性分析和随机森林分析显示,氮处理下影响毛竹叶凋落物分解的关键因子是BG、PER、pH、微生物生物量碳(MBC)和POX等。冗余分析(RDA)和回归拟合分析表明,POC与凋落物分解的质量损失率、MBC、BG、CBH、POX和PER呈显著负相关,与铵态氮(NH₄⁺-N)和硝态氮(NO₃^--N)呈显著正相关;而MAOC则与凋落物质量损失率、pH、MBC、CBH、NAG、POX和PER呈显著正相关,与微生物生物量氮(MBN)呈显著负相关。综上,氮沉降主要通过降低有关胞外酶活性抑制毛竹叶凋落物的分解,进而增加土壤POC含量,并减少MAOC含量。

关键词: 氮沉降, 毛竹林, 凋落物分解, 颗粒态有机碳, 矿物结合态有机碳

Abstract: Soil organic carbon turnover and stabilization are closely related to nitrogen deposition and litter decomposition. However, there are great uncertainties about how the decomposition of bamboo litter driven by nitrogen deposition affects soil organic carbon components. To investigate the effects of nitrogen deposition-driven litter decomposition on soil organic carbon components, we conducted an experiment at the Anji Moso bamboo ecosystem research station of Zhejiang A&F University with nitrogen treatments (N, 50 kg N·hm^-2·a^-1; CK, control with equal amount of water) and litter treatments (L, litter retention; LR, litter removal) to analyze changes in litter mass loss, soil physicochemical properties, particulate organic carbon (POC), mineral-associated organic carbon (MAOC), and soil extracellular enzyme activity (EEAs). The results showed that nitrogen application significantly reduced the mass loss of leaf litter. Nitrogen application significantly increased POC content and decreased MAOC content, but litter retention significantly increased the contents of POC and MAOC in soil. Nitrogen application significantly decreased the activities of β-1,4-glucosidase (BG), β-1,4-xylosidase (BX), cellobiohydrolase (CBH), β-1,4-N-acetyl-glucosaminnidase (NAG), phenol oxidase (POX), and peroxidase (PER), while litter retention significantly increased the activities of BG, POX, and PER. Results of correlation analysis and random forest analysis showed that the key factors affecting the decomposition of Moso bamboo litter under nitrogen treatment were BG, PER, pH, microbial biomass carbon (MBC) and POX. Through redundancy analysis (RDA) and regression fitting analysis, we found that POC was significantly negatively correlated with mass loss, MBC, BG, CBH, POX and PER, and significantly positively correlated with ammonium nitrogen (NH₄⁺-N) and nitrate nitrogen (NO₃^--N). MAOC was significantly positively correlated with mass loss, pH, MBC, CBH, NAG, POX and PER, and negatively correlated with microbial biomass nitrogen (MBN). In conclusion, nitrogen deposition inhibits bamboo leaf litter decomposition by reducing extracellular enzyme activities, thereby increasing soil POC content and decreasing MAOC content.

Key words: nitrogen deposition, bamboo forest, litter decomposition, particulate organic carbon, mineral-associated organic carbon

姜铭楷, 马书琴, 熊艳云, 吴逸卿, 吴淑倩, 钱金瑶, 陈有超, 蔡延江. 氮沉降驱动下凋落物分解对毛竹林土壤有机碳组分的影响[J]. 应用生态学报, 2024, 35(11): 2983-2991.

JIANG Mingkai, MA Shuqin, XIONG Yanyun, WU Yiqing, WU Shuqian, QIAN Jinyao, CHEN Youchao, CAI Yanjiang. Impact of litter decomposition driven by nitrogen deposition on the soil organic carbon fractions in a Moso bamboo forest[J]. Chinese Journal of Applied Ecology, 2024, 35(11): 2983-2991.

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氮沉降驱动下凋落物分解对毛竹林土壤有机碳组分的影响

Impact of litter decomposition driven by nitrogen deposition on the soil organic carbon fractions in a Moso bamboo forest

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