干旱胁迫下接种丛枝菌根真菌对七子花非结构性碳水化合物积累及C、N、P化学计量特征的影响

doi:10.13287/j.1001-9332.202204.014

应用生态学报 ›› 2022, Vol. 33 ›› Issue (4): 963-971.doi: 10.13287/j.1001-9332.202204.014

干旱胁迫下接种丛枝菌根真菌对七子花非结构性碳水化合物积累及C、N、P化学计量特征的影响

李月灵^1,2, 金则新^1,2*, 罗光宇^1,2,3, 陈超^1,2,3, 孙中帅^1,2, 王晓燕^1,2

¹台州学院浙江省植物进化生态学与保护重点实验室, 浙江台州 318000;
²台州学院生态研究所, 浙江台州 318000;
³上海师范大学生命科学学院, 上海 200234

收稿日期:2021-08-02 接受日期:2022-02-15 出版日期:2022-04-15 发布日期:2022-10-15
通讯作者: * E-mail: jzx@tzc.edu.cn
作者简介:李月灵, 男, 1989年生, 副教授。主要从事濒危植物保护研究。E-mail: liyl@tzc.edu.cn
基金资助:
国家自然科学基金项目(32001113)、浙江省自然科学基金项目(LQ19C030001,LTY22C030002)和台州学院杰出青年项目(2019JQ005)资助。

Effects of arbuscular mycorrhizal fungi inoculation on non-structural carbohydrate contents and C:N:P stoichiometry of Heptacodium miconioides under drought stress

LI Yue-ling^1,2, JIN Ze-xin^1,2*, LUO Guang-yu^1,2,3, CHEN Chao^1,2,3, SUN Zhong-shuai^1,2, WANG Xiao-yan^1,2

¹Zhejiang Provincial Key Laboratory of Plant Evolutionary and Conservation, Taizhou University, Taizhou 318000, Zhejiang, China;
²Institute of Ecology, Taizhou University, Taizhou 318000, Zhejiang, China;
³School of Life Sciences, Shanghai Normal University, Shanghai 200234, China

Received:2021-08-02 Accepted:2022-02-15 Online:2022-04-15 Published:2022-10-15

摘要/Abstract

摘要： 以濒危植物七子花二年生幼苗为研究材料,采用盆栽试验方法,研究干旱胁迫和接种丛枝菌根真菌(AMF)处理对幼苗不同器官C、N、P化学计量关系和非结构性碳水化合物(NSC)含量的影响。试验共设计4个处理:对照(CK)、干旱胁迫(D)、接种AMF(AMF)、干旱胁迫和接种AMF(D+AMF)。结果表明: 在干旱胁迫下七子花根系AMF的侵染率显著下降,但接种AMF处理植株的株高、叶片数显著高于未接种处理。接种AMF显著提高了干旱胁迫下植株根、叶可溶性糖和NSC含量及茎、叶淀粉含量,且茎和叶可溶性糖与淀粉比显著下降。干旱胁迫导致植株C含量在根和叶中显著增加,P含量在茎中显著减少;与干旱胁迫相比,胁迫下接种AMF植株根、茎、叶P含量及叶C含量显著提高,而根C、N含量及茎C含量显著降低。胁迫下接种AMF植株根、茎C∶N、C∶P、N∶P和叶N∶P均显著低于单一胁迫处理。NSC与C∶N∶P计量比的相关性分析表明,根、叶P含量与可溶性糖和NSC含量呈显著正相关,茎P含量与淀粉和NSC含量呈显著正相关,各器官N∶P与NSC含量呈显著负相关。综上,干旱胁迫显著抑制了七子花幼苗的生长,接种AMF通过提高植株根和叶的可溶性糖含量、根的可溶性糖/淀粉,增加地上部分淀粉含量,促进P元素吸收和降低各器官N∶P来增强植株耐旱性,从而提高七子花幼苗在干旱环境中的存活率。

关键词: 七子花, 干旱胁迫, 丛枝菌根真菌, 非结构性碳水化合物, C∶N∶P计量比

Abstract: A pot experiment was conducted to investigate the effects of drought stress and arbuscular mycorrhizal fungi (AMF) inoculation on C:N:P stoichiometry and non-structural carbohydrate (NSC) contents in two-year-old Heptacodium miconioides seedlings. There were four treatments, including control (CK), drought stress (D), AMF inoculation (AMF), and combined drought stress and AMF inoculation (D+AMF). The results showed that drought stress significantly reduced AMF colonization rate, whereas plant height and leaf number of inoculated treatment were significantly higher than the non-inoculated treatment. Inoculation with AMF significantly increased soluble sugar and NSC content in root and leaf, as well as starch content in stem and leaf. The inoculation significantly decreased the stem and leaf soluble sugar to starch ratio under drought stress. Drought stress caused a significant increase in C content in roots and leaves, and a significant decrease in P content in stems. Compared with no inoculation drought stress, P content in roots, stems, leaves, and C content in leaves of mycorrhizal seedlings were significantly increased by inoculation under drought stress, whereas root C and N content and stem C content were significantly reduced. Under drought stress, AMF inoculation significantly decreased C:N, C:P, and N:P ratios in roots and stems, and N:P ratios in leaves of H. miconioides. P content in roots and leaves were significantly positively correlated with soluble sugar and NSC content. Stem P content was significantly positively correlated with starch and NSC content. N:P ratios in each organ was significantly negatively correlated with NSC content. In all, inoculation with AMF can improve the drought tolerance of H. miconioides seedling by increasing soluble sugar content in roots and leaves and the soluble sugar/starch ratio in roots, improving starch content in above-ground organs, promoting the P absorption, and reducing N:P ratios in each organ. Therefore, AMF colonization could improve the survival rate of H. miconioides seedling in dry environments.

Key words: Heptacodium miconioides, drought stress, arbuscular mycorrhizal fungi, non-structural carbohydrate, C:N:P stoichiometry

李月灵, 金则新, 罗光宇, 陈超, 孙中帅, 王晓燕. 干旱胁迫下接种丛枝菌根真菌对七子花非结构性碳水化合物积累及C、N、P化学计量特征的影响[J]. 应用生态学报, 2022, 33(4): 963-971.

LI Yue-ling, JIN Ze-xin, LUO Guang-yu, CHEN Chao, SUN Zhong-shuai, WANG Xiao-yan. Effects of arbuscular mycorrhizal fungi inoculation on non-structural carbohydrate contents and C:N:P stoichiometry of Heptacodium miconioides under drought stress[J]. Chinese Journal of Applied Ecology, 2022, 33(4): 963-971.

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干旱胁迫下接种丛枝菌根真菌对七子花非结构性碳水化合物积累及C、N、P化学计量特征的影响

Effects of arbuscular mycorrhizal fungi inoculation on non-structural carbohydrate contents and C:N:P stoichiometry of Heptacodium miconioides under drought stress

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