闽楠天然居群亲子代遗传多样性及交配系统分析

doi:10.13287/j.1001-9332.202510.008

应用生态学报 ›› 2025, Vol. 36 ›› Issue (10): 3078-3084.doi: 10.13287/j.1001-9332.202510.008

闽楠天然居群亲子代遗传多样性及交配系统分析

王云鹏¹, 周志春¹, 范辉华^2*, 汤行昊², 潘昕^3,4

¹中国林业科学研究院亚热带林业研究所, 全省林木育种重点实验室, 杭州 311400;
²福建省林业科学研究院, 福州 350012;
³福建省林木种苗总站, 福州 350003;
⁴福建农林大学林学院, 福州 350002

收稿日期:2025-07-21 修回日期:2025-08-29 发布日期:2026-05-04
通讯作者: *E-mail: jofhh@163.com
作者简介:王云鹏, 男, 博士, 助理研究员。主要从事林木遗传改良研究。E-mail: wypcnsd@163.com
基金资助:
福建省种业创新与产业化工程项目(ZYCX-LY-2021005)和福建省林木种苗科技攻关七期项目(ZMGG-0708)

Analysis of parent-offspring genetic diversity and mating system in natural populations of Phoebe bournei

WANG Yunpeng¹, ZHOU Zhichun¹, FAN Huihua^2*, TANG Xinghao², PAN Xin^3,4

¹Zhejiang Key Laboratory of Forest Genetics and Breeding, Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China;
²Fujian Academy of Forestry Sciences, Fuzhou 350012, China;
³Forest Seed and Seedling General Station of Fujian Province, Fuzhou 350003, China;
⁴College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2025-07-21 Revised:2025-08-29 Published:2026-05-04

摘要/Abstract

摘要： 本研究以福建省内2个不同大小的闽楠天然居群(建瓯和顺昌)为研究对象,采用16对SSR引物对闽楠78个亲本和16个亲本的480个子代进行基因分型,估算子代的交配系统参数及亲本和子代的遗传多样性参数。结果表明:闽楠子代群体的遗传多样性(H_e=0.71)略低于亲本群体(H_e=0.80),但差异不显著,表明遗传多样性较为稳定;子代群体和亲本群体的平均观测杂合度均高于期望杂合度,表明子代与亲本群体主要以杂合子为主。交配系统参数表明,闽楠的异交率较高(t_m=1.200),双亲近交水平较低(t_m-t_s=0.195),其中面积较大的顺昌居群双亲近交系数(0.209)大于面积较小的建瓯居群(0.089),并且顺昌居群的花粉供体存在部分近亲关系。总体上,2个居群花粉供体不存在近亲关系(r_p(s)-r_p(m)=-0.051),有效花粉供体数较低(N_ep=1.40)。综上,闽楠的天然居群子代群体基本保持了亲本所具有的高遗传多样性;闽楠天然居群具有较高的异交率,居群内局部存在近交现象,但近交水平较低。当前生境片段化尚未对闽楠交配系统产生严重的负面影响,建议减少人为干扰和生境破坏,维持居群自然更新能力,促进亚居群间花粉传播,降低遗传漂变的影响。

关键词: 闽楠, 天然居群, 交配系统, 遗传多样性, SSR

Abstract: Taking two natural populations of Phoebe bournei of different sizes in Fujian Province (Jian’ou and Shunchang) as the research objects, we used 16 pairs of SSR primers to genotype 78 parents and 480 offspring from 16 parents, and estimated the mating system parameters of the offspring and the genetic diversity parameters of the parents and offspring. The results showed that the genetic diversity of the offspring population (H_e=0.71) was slightly lower than that of the parents (H_e=0.80), but the difference was not significant, indicating stable genetic diversity. The average observed heterozygosity of both the offspring and parent populations was higher than the expected heterozygosity, indicating that offspring and parent populations were primarily heterozygous. There was a high outcrossing rate (t_m=1.200) and a low level of biparental inbreeding (t_m-t_s=0.195) in P. bournei. The biparental inbreeding coefficient was higher in the larger Shunchang population (0.209) than in the smaller Jian’ou population (0.089), while partial kinship was observed among pollen donors in the Shunchang population. Overall, there was no kinship between pollen donors in the two populations (r_p(s)-r_p(m)=-0.051), and the effective number of pollen donors was low (N_ep=1.40). In conclusion, the offspring of P. bournei natural populations largely maintained high genetic diversity of the parents. The natural populations of P. bournei had a high outcrossing rate, with localized inbreeding within populations and low inbreeding level. Current habitat fragmentation had not yet severely negatively impacted the mating system of P. bournei. It was recommended to reduce human disturbance and habitat destruction, maintain the natural regeneration capacity of populations, promote pollen dispersal among subpopulations, and reduce the effects of genetic drift.

Key words: Phoebe bournei, natural population, mating system, genetic diversity, SSR

王云鹏, 周志春, 范辉华, 汤行昊, 潘昕. 闽楠天然居群亲子代遗传多样性及交配系统分析[J]. 应用生态学报, 2025, 36(10): 3078-3084.

WANG Yunpeng, ZHOU Zhichun, FAN Huihua, TANG Xinghao, PAN Xin. Analysis of parent-offspring genetic diversity and mating system in natural populations of Phoebe bournei[J]. Chinese Journal of Applied Ecology, 2025, 36(10): 3078-3084.

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闽楠天然居群亲子代遗传多样性及交配系统分析

Analysis of parent-offspring genetic diversity and mating system in natural populations of Phoebe bournei

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