丹麦森林土壤反硝化作用的动力学分析

应用生态学报 ›› 1998, Vol. 9 ›› Issue (2): 163-167.

丹麦森林土壤反硝化作用的动力学分析

于克伟¹, 陈冠雄¹, StenStruwe², AnneliseKjller²

1. 中国科学院沈阳应用生态研究所, 沈阳110015;
2. 哥本哈根大学基础微生物系, DK-1307丹麦

收稿日期:1997-08-14 修回日期:1997-10-23 出版日期:1998-03-25 发布日期:1998-03-25
基金资助:
中国-丹麦科技合作DANIDA(1995～1996)资助项目

Dynamics of denitrification potential in a Danish forest soil

Yu Kewei¹, Chen Guanxiong¹, Sten Struwe², Annelise Kjøller²

1. Institute of Applied Ecology, Academia Sinica, Shenyang 110015;
2. University of Copenhagen, DK-1307 Denmark

Received:1997-08-14 Revised:1997-10-23 Online:1998-03-25 Published:1998-03-25

摘要/Abstract

摘要： 本项研究将乙炔和氯霉素抑制技术结合起来,对丹麦一森林土壤的反硝化作用进行了研究,并考察温度对其还原酶活性的影响.反硝化还原酶活性和合成过程受O₂的抑制,厌氧培养时,需要一定时间消耗系统中残余的O₂来解除这种抑制作用.在无抗生素抑制蛋白质合成时,硝酸还原酶只有少量合成,而N₂O还原酶却显著地诱导产生.这一结果对土壤吸收N₂O能力的研究具有重要意义.在各处理下,系统中未发生亚硝酸盐的明显积累,表明亚硝酸还原酶活性大于硝酸还原酶.外加葡萄糖加速了反硝化作用,并能促进酶的合成和消除还原过程中的电子竞争.供试土壤表现出很强的厌氧呼吸作用,并受外加C源的促进.反硝化作用的活化能低于土壤厌氧呼吸的活化能,因此反硝化作用的Q₁₀值较低,CO₂和N₂O的产生比例随温度升高而加大.

关键词: 反硝化作用, N₂O还原, 动力学, 活化能

Abstract: The denitrification potential of a Danish forest soil is studied by the combination of acetylene and chloramphenicol inhibition techniques, and the effect of temperature on its related reduction enzyme activities is investigated.The activities and synthesis of reduction enzymes are inhibited by oxygen. It takes some time to consume the O₂ left in an anaerobic incubation to release this inhibition.When protein synthesis is not inhibited by antibiotic, nitrate reductase is synthesized slightly, while N₂Oreductase is significantly induced, which is of significance to study the N₂O absorbing capacity of soil. Nitrite is not considerably accumulated in all treatments, indicating that the activity of nitrite reductase is higher than nitrate reductase. Denitrification is stimulated by adding glucose, while the synthesis of enzymes and the releasing of electron competitions in the reduction processes are probably promoted as well. There shows a strong anaerobic respiration in the sampled soil, which is stimulated by Csource addition. The activation energy for denitrification is lower than that for anaerobic respiration, therefore, the Q₁₀ value for denitrification is lower, and the ratio between CO₂ and N₂Oevolution is higher with temperature.

Key words: Denitrification, N₂O reduction, Dynamics, Activation energy

于克伟, 陈冠雄, StenStruwe, AnneliseKjller. 丹麦森林土壤反硝化作用的动力学分析[J]. 应用生态学报, 1998, 9(2): 163-167.

Yu Kewei, Chen Guanxiong, Sten Struwe, Annelise Kjøller. Dynamics of denitrification potential in a Danish forest soil[J]. Chinese Journal of Applied Ecology, 1998, 9(2): 163-167.

参考文献

1 Blackmer,A.M.and Bremner,J.M.1976.Potential of soil as a sink for at mospheric nitrous oxide.Geophys.Res.L ett.,3:739～742.

2 Cicerone,R.J.1987.Changes in stratospheric ozone.Science,237:35～42.

3 Cicerone,R.J.1989.Analysis of sources and sinks of at mospheric nitrous oxide(N₂O).J.Geophys.Res.,94:18265～18271.

4 Dendooven,L.,Splatt,S.and Andersson,J.M.1994.The use of chloramphenicol in t he study of the denitrification process:some side effects.Soil Biol.Biochem.,26:925～927.

5 Donoso,L.,Santana,R.and Sanhueza,E.1993.Seasonal variation of N₂O fluxes at a tropical savannahsite:soil consumption of N₂O during the dry season.Geophys.Res.L ett.,20:1379～1382.

6 Intergovernmental Panel on Climate Change(IPCC).1994.Climate Change 1994:Radiative forcing of climate change and an evaluation of t he IPCC IS92 emission scenarios.Houghton,J.T.et al.Eds.Cambridge,U K.

7 Smith,M.S.and Tiedje,J.M.1979.Phases of denitrification following oxygen depletion in soil.SoilBiol.Biochem.,11:261～267.

8 Tanner,R.L.1990.Sources of acids,bases,and the irprecursors in the at mosphere.In:Acidic Precipitation 3:Sources,Deposition,and Canopy Interactions.Adriano,D.and Johnson,A.,Eds.Springer,New York,1～19.

9 Tiedje,J.M.,Sexstone,A.J.,Parkin,T.B.et al.1984.Anaerobic processes in soil.Plant and Soil,76:197～212.

10 Tiedje,J.M.,Simkins,S.and Groffman,P.M.1989.Perspective on measurement of denitrification in the field including recommended protocols for acetylene based methods.In:Developments in Plants and Soil Science.Clarholm,M.and Bergstrom,L.,Eds.,39:217～240.

11 Wang,W.C.,Yung,Y.L.,Lacis,A.A.et al.1976.Greenhouse effects due to manmade perturbations of trace gases.Science,194:685～690.

12 Westermann,P.and Ahring,B.K.1987.Dynamics of methane production,sulfate reduction,and denitrification in a permanently waterlogged alder swamp.A ppl.Envi ron.Microbiol.,53:2554～2559.

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