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应用生态学报 ›› 1999, Vol. 10 ›› Issue (1): 71-73.

• 研究论文 • 上一篇    下一篇

棉苗微生态学研究Ⅰ.正常棉苗内生菌分离和某些生化特性测定

赵发清, 马海燕   

  1. 南昌大学生物科学工程系, 南昌 330047
  • 收稿日期:1996-06-24 修回日期:1996-09-10 出版日期:1999-01-25 发布日期:1999-01-25
  • 通讯作者: 赵发清,男,36岁,博士,副教授,主要从事分子生态学研究,发表学术论文10余篇.
  • 基金资助:

    国家自然科学基金资助项目(39360042).

Microecology of cotton seedlings I. Endophyte isolation and measurement of some biochemical characters of normal cotton seedling

Zhao Faqing, Ma Haiyan   

  1. Nanchang University, Nanchang 330047
  • Received:1996-06-24 Revised:1996-09-10 Online:1999-01-25 Published:1999-01-25

摘要: 从4个不同棉花品种体内分离到内生菌503株,鉴定了102株,分别属于假单胞杆菌属、黄单胞杆菌属、芽胞杆菌属和欧文氏菌属。分3个不同生长期测定了4个品种的可溶性蛋白含量和超氧化物歧化酶(SOD)、过氧化物酶(POD)活性变化。结果表明,4个品种棉花SOD活性在种期(S1)、芽期(S2)和苗期(S3)呈下降趋势;不同品种、不同生长期的可溶性蛋白含量、POD活性存在极显着差异,品种与生长期还存在显着互作效应。

关键词: 棉花, 微生态学, 内生菌, 超氧化物歧化酶, 过氧化物酶

Abstract: 503 strains of endophytic bacteria were isolated from four cotton varieties after a strict surface-sterilization among which, 102 strains were subordinate to Pseudomonas, Erwinia, Xanthomoas and Bacillus genera. The concentration of soluble proteins and the activities of superoxide dismutases (SOD) and peroxidases(POD) in the four cotton varieties were measured at three different growth stages, and the results show that the SOD activities tended to decrease from seeds, sprouts to seedlings. A significant difference of eigher soluble protein concentration or SOD activity was found among different varieties and different growth stages, and there was an interaction between cotton variety and growth stage.

Key words: Cotton, Microecology, Endophyte, Superoxide dismutase, Peroxidase