大豆根茬木霉腐解产物的鉴定及其化感作用的研究

应用生态学报 ›› 2002, Vol. ›› Issue (10): 1295-1299.

大豆根茬木霉腐解产物的鉴定及其化感作用的研究

韩丽梅^1,2, 沈其荣¹, 王树起², 王旭明², 杨振明²

1. 南京农业大学资源与环境科学学院, 南京, 210095;
2. 解放军军需大学农副业生产系, 长春, 130062

收稿日期:2001-10-27 修回日期:2002-04-23 出版日期:2002-10-15
通讯作者: 韩丽梅,女,1963年生,在读博士,副教授,主要从事逆境植物营养生态方面的研究,发表论文30余篇.E-mail:Caiyunzhuiyue23@sohu.com
基金资助:
国家自然科学基金(39830220);国家“九五”科技攻关重中之重资助项目(950103).

HAN Limei^1,2, SHEN Qirong¹, WANG Shuqi², WANG Xuming², YANG Zhenming²

1. College of Natural Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095;
2. The Quartermaster University of PLA, Changchun 130062, China

Received:2001-10-27 Revised:2002-04-23 Online:2002-10-15

摘要/Abstract

摘要： 采用不同极性溶剂环己烷、乙酸乙酯、正丁醇连续提取及XAD-4树脂提取大豆根茬木霉腐解产物,并对各提取组分进行了化感作用研究,对化感作用明显的组分用GC-MS进行了化学成分分析.结果表明,大豆根茬木酶腐解产物的2周、4周环己烷、乙酸乙酯、XAD-4树脂提取组分能显著抑制初期大豆种子萌发(21h),随大豆种子萌发时间的延长,抑制作用减弱.2周、4周根茬各提取组分对大豆种子萌发中的胚根生长,所表现出的化感抑制作用规律是:0.50、1.00gDW·ml^-1乙酸乙酯、XAD-4树脂提取组分与0.10、0.25gDW·ml^-1乙酸乙酯、XAD-4树脂组分及环己烷提取组分胚根长的RI值(化感作用效应指数)差异达显著或极显著水平.乙酸乙酯、正丁醇、XAD-4树脂提取组分中的有机化合物种类有:有机酸、酚、醛、酮、苯、胺、腈、酯等,其中大多为化感物质.

关键词: 大豆, 根茬, 腐解产物, 化感作用, 木霉, 连作

Abstract: Different solvents including cyclohexane, ethyl acetate, n-butanol and XAD-4 resin were used to extract the allelochemicals from the decomposing products of soybean stubs. The results showed that the germination of soybean seeds were inhibited at initial stages by the solution extracted with cyclohexane, ethyl acetate,n-butanol and resin from the decomposing products of soybean stubs that had been incubated for 2 and 4 weeks. The inhibition was gradually weakened with the time. When concentrations of the ethyl acetate and resin fractions were 0.50, 1.00gDW穖l^-1and when their concentrations were 0.10, 0.25gDW穖l^-1,their RI (the index of allelopathic effect) value of soybean radicle length reached significant level. The RIvalues of the former and those of the cyclohexane fractions reached significant level also. The chemical constituents of allelopathic fractions were identified by GC-MSas organic acids, phenol, aldehyde, ketone, benzene, amine, nitrile, ester and some other compounds, some of which were allelochemicals.

中图分类号:

S181

韩丽梅, 沈其荣, 王树起, 王旭明, 杨振明. 大豆根茬木霉腐解产物的鉴定及其化感作用的研究[J]. 应用生态学报, 2002, (10): 1295-1299.

HAN Limei, SHEN Qirong, WANG Shuqi, WANG Xuming, YANG Zhenming. [J]. Chinese Journal of Applied Ecology, 2002, (10): 1295-1299.

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