污灌对小麦幼苗生长及活性氧代谢的影响

应用生态学报 ›› 2002, Vol. ›› Issue (10): 1319-1322.

污灌对小麦幼苗生长及活性氧代谢的影响

刘登义, 王友保, 张徐祥, 司琴

安徽师范大学生命科学学院, 芜湖, 241000

收稿日期:2001-10-29 修回日期:2002-06-26 出版日期:2002-10-15
通讯作者: 王友保
基金资助:
国家教育部优秀青年教师基金;安徽师范大学专项基金助项目(150076).

LIU Dengyi, WANG Youbao, ZHANG Xuxiang, SI Qin

College of Life Science, Anhui Normal University, Wuhu 241000

Received:2001-10-29 Revised:2002-06-26 Online:2002-10-15

摘要/Abstract

摘要： 通过水培的方式研究了污水灌溉对小麦幼苗生长及活性氧代谢系统的影响.结果表明,经污水浇灌的小麦幼苗与对照组相比,植株矮小,根短,根数目少,茎、叶、根的干重、鲜重和可溶性蛋白含量均明显减少,并出现叶尖枯黄,叶片色素含量下降,叶绿素a/b与电导率明显上升现象,几种色素对污灌的敏感性为叶绿素b>叶绿素a>类葫萝卜素.同时MDA水平上升,而SOD、POD和CAT活性显著下降.其中SOD对环境胁迫最敏感.三者活性分别下降19.1%～33.1%、37.8%～47.8%和30.2%～35.9%.

关键词: 污灌, 小麦, 幼苗生长, 活性氧代谢

Abstract: The effects of sewage irrigation on the seedling growth of wheat and its activate oxygen metabolism were studied by water cultivation experiments.The results showed that compared with control,the wheat under sewage irrigation was short and small, and its roots number,fresh /dry weight of stem or root,and content of soluble protein decreased drastically.The content of chlorophyll decreased, while the Cyta/Cytb and membrane lipid peroxidation were increased. The sensitivity of pigment content of wheat leaf undr sewage irrigation was in order of Chlorophyll b > Chlorophyll a >Carotenoid.Furthermore, the POD, SOD and CAT activities of the wheat went down in varying degrees,and the SODwas the most susceptible enzyme to waste water stress. In this paper, the activities of POD, SODand CATdecreased in19.1%～33.1%,37.8%～47.8% and 30.2%～ 35.9%,respectively.

中图分类号:

X171.5

刘登义, 王友保, 张徐祥, 司琴. 污灌对小麦幼苗生长及活性氧代谢的影响[J]. 应用生态学报, 2002, (10): 1319-1322.

LIU Dengyi, WANG Youbao, ZHANG Xuxiang, SI Qin . [J]. Chinese Journal of Applied Ecology, 2002, (10): 1319-1322.

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