用ISSR标记检测黄麻野生种与栽培种遗传多样性

应用生态学报 ›› 2003, Vol. ›› Issue (9): 1473-1477.

用ISSR标记检测黄麻野生种与栽培种遗传多样性

祁建民¹, 周东新², 吴为人¹, 林荔辉¹, 吴建梅¹, 方平平¹

1. 福建农林大学生命科学学院, 福州 350002;
2. 福建省龙岩市烟草科学研究所, 龙岩 364000

收稿日期:2003-03-18 修回日期:2003-06-06
通讯作者: 祁建民,男,1948年出生,副研究员,硕士导师,主要从事麻类遗传育种与生物多样性研究,发表论文50余篇.Tel:0591-3789362,E-mail:Qijm863@sina.com.
基金资助:
国家高技术研究发展计划资助项目(2001AA241212)

Application of ISSR technology in genetic diversity detection of Jute

QI Jianmin¹, ZHOU Dongxin², WUWeiren¹, LIN Lihui¹, WU Jianmei¹, FANG Pingping ¹

1. College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Longyan Tobacco Branch Company, Longyan 364000, China

Received:2003-03-18 Revised:2003-06-06

摘要/Abstract

摘要： 利用ISSR标记对黄麻属(Corchorus)10个种27份材料的遗传多样性进行分析,25个ISSR引物共扩增出283条带,平均每个引物扩增出10.48条带,多态性条带比例(PPB)为92.85%;种间遗传相似系数在0.33～0.97之间,表现出丰富的遗传多样性。系统聚类结果显示,第Ⅰ、Ⅲ类群均为黄麻属8个种11份原始野生种,种间存在丰富的遗传多样性;第Ⅱ类群为黄麻两个栽培种及其近缘野生种,共16份材料,种遗传相似性较高;基因组聚类结果与经典分类相符。利用分子标记技术研究初步可以认为,荨麻叶种为最原始的黄麻野生种之一;三室种21C为三室种的一个变种;甜麻为一个尚待定性的野生种。同组材料的RAPD和ISSR分析结果,具有较高的相似性和可信度。

关键词: 黄麻, ISSR, 遗传多样性, 亲缘关系

Abstract: The genetic diversity among 27 accessions of Corchorus, including 10 Jute species, was investigated with ISSR technique.283 DNA bands were amplified with 25 ISSR primers, among which, 263 (92.85%) were polymorphic, with 10.48 bands per primer in average.Afurther systemic cluster analysis indicated that the accessions could be clustered into three groups, and the group Ⅱ (including two cultispecies and their close wild species) was obviously genetically different from the groups I and Ⅲ (including eight wild species).Moreover, 16 accessions in group Ⅱ presented a higher intraspecific genetic resemblance, while 11 accessions among groups I & Ⅲ showed an abundant interspecific genetic diversity.After synthesized the relevant findings of morphology and DNA classification, it's found that C.urticifolius could be one of the original wild species, C.tilaculariszic was a variation of C.tilaculari, and Tian Jute could be an untitled wild species.

Key words: Jute, ISSR, Genetic diversity, Consanguinity

中图分类号:

Q941

祁建民, 周东新, 吴为人, 林荔辉, 吴建梅, 方平平. 用ISSR标记检测黄麻野生种与栽培种遗传多样性[J]. 应用生态学报, 2003, (9): 1473-1477.

QI Jianmin, ZHOU Dongxin, WUWeiren, LIN Lihui, WU Jianmei, FANG Pingping . Application of ISSR technology in genetic diversity detection of Jute[J]. Chinese Journal of Applied Ecology, 2003, (9): 1473-1477.

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