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高温胁迫对切花菊‘神马’光合作用与叶绿素荧光的影响

孙宪芝;郑成淑;王秀峰   

  1. 山东农业大学园艺科学与工程学院作物生物学国家重点实验室, 山东泰安 271018
  • 收稿日期:2008-01-24 修回日期:1900-01-01 出版日期:2008-10-20 发布日期:2008-10-20

Effects of high temperature stress on photosynthesis and chlorophyll fluorescence of cut flower chrysanthemum (Dendranthema grandiflora ‘Jinba’).

SUN Xian-zhi; ZHENG Cheng-shu; WANG Xiu-feng   

  1. State Key Laboratory of Crop Biology, College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271018, Shandong, China
  • Received:2008-01-24 Revised:1900-01-01 Online:2008-10-20 Published:2008-10-20

摘要: 以切花菊品种‘神马’为试材,在40 ℃/35 ℃(昼/夜)与33 ℃/28 ℃高温下分别胁迫11 d,然后转入23 ℃/18 ℃恢复5 d,研究不同高温强度及恢复对菊花光合作用的影响.结果表明:33 ℃/28 ℃下净光合速率(Pn)逐渐降低,气孔导度(Gs)5 d后明显降低,两参数均可在23 ℃/18 ℃下恢复到对照的80%以上;40 ℃/35 ℃高温下PnGs大幅度持续降低,胁迫前期,胞间CO2浓度(Ci)上升,Pn的降低主要由非气孔因素导致;9 d后CiPn同时降低,气孔限制成为Pn 降低的主要因素.高温使菊花叶片的PSⅡ潜在活性(Fv/Fo)、最大光化效率(Fv/Fm)、实际光化效率(ΦPSⅡ)与天线转换效率 (Fv′/Fm′)降低,天线热耗散(D)增加,表明高温胁迫下菊花通过降低光能的捕获与通过PSⅡ的电子传递效率来保护反应中心免受伤害.33 ℃/28 ℃下光化学猝灭系数(qP)呈先下降后上升趋势,推测此温度下受体端电子传递首先受到抑制;40 ℃/35 ℃下qP持续增加,表明放氧复合体(OEC)可能是菊花光合系统中极端高温伤害的原初位点.

关键词: 生物炭, 植株含磷量, 土壤有效磷, Meta分析, 土壤磷酸酶

Abstract: Cut flower chrysanthemum (Dendranthema grandiflora ‘Jinba’) plants were treated with 40 ℃/35 ℃ or 33 ℃/28 ℃ (day/night) for 11 days and then transferred to 23 ℃/18 ℃ for 5 days to study the changes in their photosynthesis and fluorescence parameters under high temperature stress and normal temperature recovery. The results showed that on the 5th day of 33 ℃/28 ℃ treatment, net photosynthesis (Pn) decreased gradually and stomatal conductance (Gs) decreased evidently; while after recovery for 5 days, both Pn and Gs resumed to 80% of the control. At 40 ℃/35 ℃,Pn and Gs decreased dramatically. The increase of intercellular CO2 concentration (Ci) at the early stage under given high temperatures showed that the photosynthesis inhibition by high temperature stress was resulted from non-stomatal limitations. However, 9 days later, stomatal limitation became the mainly cause of photosynthesis inhibition. The intrinsic photochemical efficiency (Fv/Fm), quantum yield of PSⅡ(ΦPSⅡ), and the efficiency of excitation energy capture by open PSⅡ reaction center(Fv′/Fm′) at 33 ℃/28 ℃ and 40 ℃/35 ℃ all decreased, with antenna heat dissipation increased, indicating that reaction center was protected by decreased light capture and efficiency of electron transfer through PSⅡ. The photochemical quenching (qP) at 33 ℃/28 ℃ descended first and turned to rise then, suggesting that the electron transfer was firstly restrained by the stress. Contrastively, qP rose continuously at 40 ℃/35 ℃, indicating that oxygen-evolving complex (OEC) was the location in chrysanthemum photosynthesis apparatus most sensitive to extreme high tempe rature.

Key words: soil available phosphorus, biochar, meta analysis, soil phosphatase, plant phosphorus concentration