交替光诱导下15种苏铁气孔特性及其对光合碳同化和水分利用效率的影响

doi:10.13287/j.1001-9332.202412.002

应用生态学报 ›› 2024, Vol. 35 ›› Issue (12): 3304-3314.doi: 10.13287/j.1001-9332.202412.002

交替光诱导下15种苏铁气孔特性及其对光合碳同化和水分利用效率的影响

刘小美^1,2, 朱俊杰^1,2*, 杨泉光³, 吴中华^1,2

¹广西大学林学院, 南宁 530004;
²广西大学亚热带农业生物资源保护与利用国家重点实验室, 南宁 530004;
³南宁植物园(南宁青秀山风景名胜旅游开发有限责任公司), 南宁 530029

收稿日期:2024-05-20 接受日期:2024-09-12 出版日期:2024-12-18 发布日期:2025-06-18
通讯作者: *E-mail: 20150067@gxu.edu.cn
作者简介:刘小美, 女, 1998年生, 硕士研究生。主要从事植物光合生理生态研究。E-mail: 2389619556@qq.com
基金资助:
国家自然科学基金项目(31860109)和青秀山管委会科学研究项目(QXS2021-05)

Alternating light-induced stomatal characteristics and their effects on photosynthetic carbon assimilation and water use efficiency among 15 Cycad species

LIU Xiaomei^1,2, ZHU Junjie^1,2*, YANG Quanguang³, WU Zhonghua^1,2

¹College of Forestry, Guangxi University, Nanning 530004, China;
²State Key Laboratory for Conservation and Utilization of Subtropical Agricultural Biological Resources, Guangxi University, Nanning 530004, China;
³Nanning Botanical Garden (Nanning Qingxiu Mountain Scenic Spots Tourism Development Co., Ltd.), Nanning 530029, China

Received:2024-05-20 Accepted:2024-09-12 Online:2024-12-18 Published:2025-06-18

摘要/Abstract

摘要： 为分析动态光诱导过程中苏铁植物的气孔响应及其对光合碳同化和水分利用效率的影响,以南亚热带青秀山同质园15种苏铁为材料,研究它们的羽叶在持续弱-强-弱交替光诱导过程中的气孔动力学特征和结构性状。结果表明: 弱光诱导转强光诱导时,15种苏铁净光合速率(A)达95%最大值的时间(A_95t)为14～38 min,以肿茎苏铁最快,摩瑞大泽米铁最慢,气孔导度(g_s)上升速率略慢于A上升速率。随后持续强光诱导达稳态时,攀枝花苏铁A值最高,叉叶苏铁最低。由强光转弱光诱导阶段,多数苏铁植物A值先大幅下降至次低值,再缓慢降至最低值;此阶段绝大多数苏铁植物g_s下降速率比由弱光转强光阶段的上升速率小。气孔动力学参数中,稳态阶段的最大g_s与强光转弱光后g_s下降的速率(Sl_max,d)之间,以及弱光转强光时g_s随时间增加的常数与A_95t之间呈显著正相关。气孔打开的滞后时间(λ_i)与气孔关闭的滞后时间,以及g_s下降到稳态的时间常数与Sl_max,d呈显著负相关。气孔结构性状中,气孔长度与λ_i呈显著正相关。主成分分析表明,最大光合速率、A_95t、气孔限制值和气孔长度对苏铁群体性状显著性差异的贡献率最大。苏铁植物光合碳同化潜力较低,但对光资源的变化更敏感,通过高效的气孔调节优化CO₂获取,同时维持较高的水分利用效率,是适应林下生活的体现。

关键词: 苏铁植物, 气孔响应动力学, 光合作用, 水分利用效率, 气孔特性

Abstract: To analyze the stomatal response of Cycad species during dynamic light induction and their effects on photosynthetic carbon assimilation and water use efficiency, we examined the stomatal dynamics and structural cha-racteristics of their pinnae in 15 Cycad species from Qingxiu Mountain Homogeneous Garden during continuous weak-strong-weak alternating light induction. The results showed that, from low to high light intensity, the duration for the photosynthetic rate (A) to reach 95% of its maximum value (A_95t) ranged from 14 minutes (Cycas pachypoda) to 38 minutes (Macrozamia moorei). The ascending rate of stomatal conductance (g_s) was slightly slower than that of A. After continuous strong light exposure, the A value of Cycas panzhihuaensis was the highest and that of Cycas bifida was the lowest. During the light transition from strong to weak, A dropped sharply, and then decreased slowly to the lowest value. Across the 15 examined cycad species, the decline rate of g_s during the decreasing light intensity stage was less steep than the rise in g_s during the intensifying light stage. For stomatal traits, the maximum g_s under normal condition was positively correlated with g_s decline rate (Sl_{max, d}) during the decreasing light intensity stage, and the constant characterizing g_s increasing rate during the weak to strong light treatment was positively correlated with A_95t. The stomatal opening duration (λ_i) was negatively correlated with the stomatal closing duration. The time constant of g_s descending to steady state was negatively correlated with Sl_max,d. Under the stomatal conductance, there was a significant positive correlation between stomatal length and λ_i. Results of principal component analysis showed that maximum photosynthetic rate, A_95t, stomatal limit value and stomatal length contributed the most to the variations in stomatal and photosynthetic traits of Cycads. Our results suggested that the photosynthetic potential of Cycad species was lower, but was sensitive to dynamic light resources, through efficient stomatal regulation. Cycad species could optimize CO₂ acquisition and maintain high water use efficiency, which is commonly observed in understory plants.

Key words: Cycad species, kinetics of stomatal response, photosynthesis, water use efficiency, stomatal characteristics

刘小美, 朱俊杰, 杨泉光, 吴中华. 交替光诱导下15种苏铁气孔特性及其对光合碳同化和水分利用效率的影响[J]. 应用生态学报, 2024, 35(12): 3304-3314.

LIU Xiaomei, ZHU Junjie, YANG Quanguang, WU Zhonghua. Alternating light-induced stomatal characteristics and their effects on photosynthetic carbon assimilation and water use efficiency among 15 Cycad species[J]. Chinese Journal of Applied Ecology, 2024, 35(12): 3304-3314.

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交替光诱导下15种苏铁气孔特性及其对光合碳同化和水分利用效率的影响

Alternating light-induced stomatal characteristics and their effects on photosynthetic carbon assimilation and water use efficiency among 15 Cycad species

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