二氧化硫对小麦的氧化胁迫及其某些信号分子的调节

应用生态学报 ›› 2005, Vol. 16 ›› Issue (6): 1038-1042.

二氧化硫对小麦的氧化胁迫及其某些信号分子的调节

郝林¹, 张惠文², 徐昕¹, 陶思源¹, 于龙¹

1. 沈阳师范大学生物系, 沈阳 110034;
2. 中国科学院沈阳应用生态研究所, 沈阳 110016

收稿日期:2004-10-12 修回日期:2005-02-28 出版日期:2005-06-15 发布日期:2005-06-15
通讯作者: 郝林,男,1963年出生,博士,教授.主要从事植物对生物和非生物胁迫应答的研究,发表论文40多篇.E-mail:haolinwj2001@yahoo.com.cn
基金资助:
国家自然科学基金项目(30070129)和辽宁省博士启动基金项目(023804111)和沈阳师范大学实验中心主任基金资助项目(SY200406).

SO₂-caused oxidative stress and modulation of some signal molecules in wheat

HAO Lin¹, ZHANG Huiwen², XU Xin¹, TAO Siyuan¹, YU Long ¹

1. Department of Biology, Shenyang Normal University, Shenyang 110034, China;
2. Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China

Received:2004-10-12 Revised:2005-02-28 Online:2005-06-15 Published:2005-06-15

摘要/Abstract

摘要： 通过在密闭的培养箱中一次性通入不同体积浓度的SO₂气体,研究了小麦幼苗超氧自由基O₂^-·含量和3种抗氧化酶活性的变化,探讨了信号分子水杨酸、乙烯和过氧化氢对SO₂氧化胁迫的调节作用.结果表明,当通入10和40μl·L^-1SO₂时,小麦叶片中O₂^-·含量递增,过氧化物酶(POD)和过氧化氢酶(CAT)活性增强,但超氧化物歧化酶(SOD)活性降低.当SO₂浓度达到50μl·L^-1时,POD和CAT活性也开始降低,此时叶片尖端出现坏死,叶片绿色部位滋生大量真菌.用1mmol·L^-1水杨酸(SA)(pH6.5)浸泡小麦干种子6h,或用10mmol·L^-1H2O₂浸泡幼苗,O₂^-·含量低于对照植株,而3种酶的活性高于对照植株,均可有效地减轻SO₂的氧化胁迫.在SO₂熏蒸下,乙烯显著抑制3种酶的活力,提高O₂^-·的形成速率.SA与乙烯同时使用时,SA几乎完全消除了乙烯的负面作用.

关键词: 二氧化硫, 小麦, 水杨酸, 乙烯, 过氧化氢

Abstract: By adding different concentration SO₂ to culture boxes,this paper studied the responses of wheat seedling's superoxide anion radical O₂^-· and antioxidant enzymes to SO₂.The results showed that when the concentration of SO₂ was 10 and 40 μl·L^-1,the O₂^-· content and peroxidase (POD) and catalase(CAT) activities of wheat leaf were increased,while the activity of superoxide dismutase(SOD) was reduced.When the SO₂ concentration was 50 μl·L^-1,the activities of POD and CAT were decreased,with the top end leaves appeared necrosis,and more fungi multiplied on green leaves.Presoaking wheat seeds with 1 mmol·L^-1 salicylic acid (SA) for 6 h or with 10 mmol·L^-1 H₂O₂ for 12 h could efficiently alleviate the oxidative stress caused by SO₂,with the O₂^-· content decreased and the activities of test antioxidant enzymes increased.Under SO₂ fumigation,ethylene significantly inhibited the activities of test enzymes,and promoted the O₂^-· production rate.When applying SA and ethylene at the same time,SA could almost completely eliminate the influences of ethylene on O₂^-· production and test enzyme activities.

Key words: SO₂, Wheat, Salicylic acid, Ethylene, Hydrogen peroxide

中图分类号:

X171

郝林, 张惠文, 徐昕, 陶思源, 于龙. 二氧化硫对小麦的氧化胁迫及其某些信号分子的调节[J]. 应用生态学报, 2005, 16(6): 1038-1042.

HAO Lin, ZHANG Huiwen, XU Xin, TAO Siyuan, YU Long . SO₂-caused oxidative stress and modulation of some signal molecules in wheat[J]. Chinese Journal of Applied Ecology, 2005, 16(6): 1038-1042.

参考文献

[1] Aebi HE. 1983. Catalase. In:Bergmeyer HU, ed. Methods of Enzymatic Analyses. Weinheim: Verlag Chemie. 273 ～282
[2] Alvarez ME. 2000. Salicylic acid in the machinery of hypersensitive cell death and disease resistance. Plant Mol Biol, 44:429～442
[3] Beauchamp C, Fridovich J. 1971. Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Anal Biochem,44:276～ 287
[4] Bleecker AB, Kende H. 2000. Ethylene: A gaseous signal molecule in plants. Ann Rev Cell Dev Biol, 16:1 ～ 18
[5] Borsani O, Valpuesta V, Botella MA. 2001. Evidence for a role of salicylic acid in the oxidative damage generated by NaCl and osmotic stress in Arabidopsis seedlings. Plant Physiol, 126:1024～ 1030
[6] Chen Z, Ricigliano W, Klessig DF. 1993. Purification and characterization of a soluble salicylic acid binding protein from tobacco. Proc Nat Acad Sci USA, 90:9533～9537
[7] Desikan R, A-H-Mackerness S, Hancock JT, et al. 2001. Regulation of the Arabidopsis transcriptome by oxidative stress. Plant Physiol, 127:159～172
[8] Ding CK, Wang CY, Gross KC, et al. 2002. Jasmonate and salicylate induce expression of pathogenesis-related protein genes and increase resistance to chilling injury in tomato fruit. Planta, 214:895～901
[9] Doke N. 1983. Involvement of superoxide anion generation in the hypersensitive response of potato tuber tissues to infection with an incompatible race of Phytophthora infestans and to the hyphal wall components. Physiol Plant Pathol, 23: 345～ 357
[10] Foyer CH, Lopez-Delgado H, Dat JF, et al. 1997. Hydrogen peroxide- and glutathione-associated mechanism of acclamatory stress tolerance and signalling. Physiol Plant, 100:241 ～254
[11] Gazzarrini S, Mccourt P. 2003. Cross-talk in plant hormone signalling: What arabidopsis mutants are telling us. Ann Bot, 91: 605～612
[12] Han S-M(韩素梅).2001.Tree stomatal infiltration and SO₂ injury and protection effect of ABA. Chin J Appl Ecol (应用生态学报),12 (3): 425 ～ 428 (in Chinese)
[13] Han Y(韩阳),Li Z-Z(李珍珍),Liu R-K(刘荣坤).2002.Potential of purifying SO₂ of main tree species and their plants quota in Shenyang area. Chin J Appl Ecol (应用生态学报), 13 (5): 601 ～604(in Chinese)
[14] Han Y(韩阳), Liu R-K(刘荣坤). 1998. Effect of phosphate buffer on membrane lipid peroxidation of plants and its relation to sulphureous acid harm. Chin J Appl Ecol (应用生态学报), 9(2):201 ～ 205 (in Chinese)
[15] Inze D, Van Montagu M. 2002. Oxidative Stress in Plants. New York: Taylor and Francis. 1 ～ 171
[16] Kunkel BN, Brooks DM. 2002. Cross talk between signaling pathways in pathogen defense. Curr Opin Plant Biol, 5: 325～ 331
[17] Lowry DH, Rosebrough HJ, Farr AL. 1951. Protein measurement with Folin phenol reagent. J Biol Chem, 193: 262 ～ 275
[18] Madamanchi NR, Alscher RG. 1991. Metabolic bases for differences in sensitivity of two pea cultivars to sulfur dioxide. Plant Physiol,97: 88～ 93
[19] Metwally A, Finkemeier I, Georgi M, et al. 2003. Salicylic acid alleviates the cadmium toxicity in barley seedlings. Plant Physiol,132:272 ～ 281
[20] Neill SJ, Desikan R, Clarke A, et al. 2002. Hydrogen peroxide and nitric oxide as signalling molecules in plants. J Exp Bot, 53:1237 ～1247
[21] Neill SJ, Desikan R, Hancock JT. 2002. Hydrogen peroxide signalling. Curr Opin Plant Biol, 5: 388 ～ 395
[22] Nouchi I. 2002. Responses of whole plants to air pollutions. In: Omasa K, ed. Air Pollution and Plant Biotechnology. Tokyo:Springer-Verlag. 4 ～ 8
[23] Prasad TK, Anderson MD, Martin BA, et al. 1994. Evidence for chilling-induced oxidative stress in maize seedlings and a regulatory role for hydrogen peroxide. Plant Cell, 6:65～74
[24] Rao MV, Paliyath G, Ormuod DP, et al. 1997. Influence of salicylic acid on H2O₂ production, oxidative stress, and H2O₂-metabolizing enzymes. Plant Physiol, 115:137 ～ 149
[25] Tanaka K, Sugahara K. 1980. Role of superoxide dismutase in defense against SO₂ toxicity and an increase in superoxide dismutase activity with SO₂ fumigation. Plant Cell Physiol, 21:601 ～611
[26] Tissa S, Darren T, Eric B, et al. 2000. Acetyl salicylic acid(aspirin)and salicylic acid induce multiple stress tolerance in bean and tomato plants. Plant Growth Regul, 30:157～ 161
[27] Vallelian-Bindeschendler L, Schweizer P, Mosinger E, et al. 1998.Heat-induced resistance in barley to powdery mildew (Blumeria graminis f. sp. hordei) is associated with a burst of active oxygen species. Physiol Mol Plant Pathol, 52:185～ 199
[28] van Camp W, van Montagu M, Inze D. 1994. Superoxide dismutases. In: Foyer C, Mullineaux P, eds. Causes of Photo-oxidative Stress in Plants and Amelioration of Defense Systems. Boca Raton:CRC Press. 317 ～ 341
[29] van Camp W, van Montagu M, Inze D. 1998. H2O₂ and NO:Redox signals in disease resistance. Trends Plant Sci, 3: 330～ 334
[30] Wang KLC, Li H, Ecker JR. 2002. Ethylene biosynthesis and signaling networks. Plant Cell, 14:131 ～ 151
[31] Willekens H, Van Camp W, Van Montagu M, et al. 1994. Ozone,sulfur dioxide, and ultraviolet B have similar effects on mRNA accumulation of antioxidant genes in Nicotiana plumbaginifolia L.Plant Physiol, 106:1007 ～ 1014
[32] Zhang Z-L (张志良). 1990. Guide to Plant Physiology Experiments. Beijing: Higher Education Press. 88 (in Chinese)