金鱼(Carassiusauratus)鳃对颗粒吸附态铅的吸收

应用生态学报 ›› 2000, Vol. ›› Issue (2): 283-286.

金鱼(Carassiusauratus)鳃对颗粒吸附态铅的吸收

刘长发¹, 陶澍¹, 曹军¹, 李本纲¹, 林健枝²

1. 北京大学城市与环境学系, 北京100871;
2. 香港中文大学地理系, 香港

收稿日期:1998-10-19 修回日期:1999-04-19 出版日期:2000-03-25
通讯作者: 陶澍
基金资助:
国家杰出青年基金资助项目(49525102)

Uptake of particulate lead via gills of goldfish

LIU Changfa¹, TAO Shu¹, CAO Jun¹, LI Bengang¹, Lam Kin Che²

1. Department of Urban and Environmental Sciences, Peking University, Beijing 100871;
2. Department of Geography, Chinese University of Hong Kong, Hong Kong

Received:1998-10-19 Revised:1999-04-19 Online:2000-03-25

摘要/Abstract

摘要： 研究了金鱼 (Carassiusauratus)鳃在悬浮水铝矿吸附态Pb暴露实验中对Pb吸收过程.结果表明,在保持水中游离态Pb浓度不变 (0.1mg·L^-1)、吸附态Pb浓度增加的条件下,鳃中Pb、Al浓度均随暴露浓度增加而增加,血液中Pb浓度随鳃中Pb浓度的增加率 (直线斜率0.171)低于溶解态Pb暴露实验的增加率 (直线斜率0.343),附着在鳃上的部分Pb未转移至血液中,鳃中Pb/Al比较水相Pb/Al比 (0.0039)高 1～2个数量级,并且在组织切片中观察到积聚在粘液上的矿物颗粒,从而证实了颗粒态Pb对鱼鳃吸收的生物有效性.金鱼鳃吸收颗粒态Pb的主要机理是:流经鳃丝表面的悬浮颗粒物吸附态Pb在鳃表面解离并被动扩散或载体转运穿过鳃部细胞进入血液循环,颗粒物在鳃丝表面短时间停留后与脱落粘液一起随水流从鳃部排出.

关键词: 铅, 金鱼, 颗粒态, 生物有效性

Abstract: This paper studied the uptake of Pb absorbed on suspended gibbsite via gills of goldfish (Carassius auratus). Under conditions of the concentration of the particulate Pb varied while that of free Pb maintained at a constant level,the levels of both Pb and Al in the gills increased with increasing exposed Pb concentration.The increasing rate (slope 0.171) of Pb level in the blood with the Pb level in the gills was lower than that (slope 0.343) of soluble Pb exposure experiment.Partial Pb adhered on the gills did not transfer into blood.The ratio of Pb/Al in the gills was 1～2 orders of magnitude higher than that in the water,and on the microtome section of filament, the gibbsite particles adhered on gill mucus were observed, indicating the bioavailability of the particulate Pb uptake by the gills of goldfish.The major mechanism was: when the absorbed Pb on suspended particles was flowed into gill filaments,it was desorbed on the surface of gills,and then, passively diffused or transferred by carriers via gill cells into flood,and after the particles stayed on the surface of gill filaments for a short time,in company with sloughed mucus,they were released from the gills along with waterflow.

Key words: Lead, Goldfish, Particulate lead, Bioavailability

刘长发, 陶澍, 曹军, 李本纲, 林健枝. 金鱼(Carassiusauratus)鳃对颗粒吸附态铅的吸收[J]. 应用生态学报, 2000, (2): 283-286.

LIU Changfa, TAO Shu, CAO Jun, LI Bengang, Lam Kin Che. Uptake of particulate lead via gills of goldfish[J]. Chinese Journal of Applied Ecology, 2000, (2): 283-286.

参考文献

1 Arillo A,Margiocco C,Melodia F.1979.The gill sialic acid content as an index of environmental stress in rainbow trout,Salmon gairdneri,Richardson.J Fish Biol,15:405～410
2　Davies NA,Taylor MG and Simkiss K.1997.The influence of particle surface characteristics on pollutant metal uptake by cells.Environ Pollut,96(2):179～184
3 Dougan WK and Wilson AL.1974.The absorptiometric determine of aluminium in water.A comparison of some chromogenic reagents and the development of an improved method.Analyst,99:413～430
4 Liu Ch-F(刘长发),Li H(李杭),Tao Sh(陶澍).1999.Uptake and accumulation of free lead by goldfish (Carassius auratus L.) and effect of gill mucus on the processes.Acta Sci Circ(环境科学学报),19(4):438～442(in Chinese)
5 Martens DW and Servizi JA.1993.Suspended sediment particles inside gills and spleens of juvenile pacific salmon (Oncorhynchus spp.).Can J Fish Aquat Sci,50:586～590
6 Newman MC and Jagoe CH.1994.Ligands and the bioavailability of metals in aquatic environments.In:Hamelink JL,Landrum PF,Bergman HL eds.Bioavailability:Physical,Chemical,and Biological Interactions.Michigan:Lewis Publishers.pp.39～61
7 Pagenkopf GK.1983.Gill surface interaction model for trace metal toxicity to fishes:role of complexation,pH,and water hardness.Environ Sci Technol,17(2):342～347
8 Playle RC and Wood,CM.1989.Water chemistry changes in the gill micro-environment of rainbow trout:Experimental theory and observation.J Comp Physiol,B159:527～537
9 Playle RC,Dixon DG and Burnison K.1993.Copper and cadmium binding to fish gills:Estimates of metal-gill stability constants and modelling of metal accumulation.Can J Fish Aquat Sci,50:2678～2687
10 Playle RC,Gensemer RW and Dixon DG 1992.Copper accumulation of gills of fathead minnows:influence of water hardness,complexation and pH of the gill micro-environment.Environ Toxicol Chem,11:381～389
11 Tao S,and Liang T.1997.Long-term monitoring of bioavailable copper in aquatic environment using a resin-filled dialysis membrane.Bull Environ Contam Toxicol,58:712～719
12 Tao S,Liang T,Liu CF,and Xu SP.1999.Uptake of copper by neontetras (Paracheirodon innesi) in the presence and absence of particulate and humic matter.Ecotoxicol,8:269～275
13 Tessier A.1992.Sorption of trace elements of natural particles in oxic environment.In:Buffle J and van Leeuwn HP eds.Environmental Particles.Michigan:Lewis Publishers.pp.425～453
14 Verbost PM,van Rooji J,Flik G et al.1989.The movement of cadmium through freshwater trout branchial epithelium and its interference with calcium transport.J Exp Biol,145:185～197

[1]	代伶俐, 赵迪. 中国稻田系统砷污染的区域驱动机制及分区精准阻控研究进展 [J]. 应用生态学报, 2026, 37(6): 2085-2093.
[2]	任建昊, 矫智慧, 朱道光, 孙晓新. 小兴安岭沼泽湿地微生物残体碳对土壤有机碳组分积累的贡献及影响因素 [J]. 应用生态学报, 2026, 37(4): 1083-1090.
[3]	吴臻杰, 谭思懿, 吴福忠, 谢荣樟, 朱晶晶, 倪祥银. 亚热带杉木和米槠人工林土壤真菌和细菌残体碳积累差异 [J]. 应用生态学报, 2026, 37(3): 779-786.
[4]	单雅茹, 田嘉禾, 樊修稳, 刘磊. 中国大陆土壤有机碳组分的空间分布及其对气候变化的响应 [J]. 应用生态学报, 2025, 36(3): 847-858.
[5]	周玥, 李娅芸, 李娜, 李会军, 张羽涵, 安韶山, 王宝荣. 黄土丘陵区降水变化下草地土壤微生物残体碳对土壤有机碳组分的贡献及其影响因素 [J]. 应用生态学报, 2024, 35(9): 2592-2598.
[6]	姜铭楷, 马书琴, 熊艳云, 吴逸卿, 吴淑倩, 钱金瑶, 陈有超, 蔡延江. 氮沉降驱动下凋落物分解对毛竹林土壤有机碳组分的影响 [J]. 应用生态学报, 2024, 35(11): 2983-2991.
[7]	李沿霖, 陈娟, 楚丽丽, 朱鹏超, 付依瑞, 黄冬琳, 张达斌, 高亚军, 王朝辉. 解锌微生物的分离、筛选及在低锌石灰性土壤的应用 [J]. 应用生态学报, 2024, 35(10): 2765-2774.
[8]	顾嘉诚, 王文敏, 王振, 李鲁华, 蒋贵菊, 王家平, 程志博. 玉米/大豆间作对根际土壤磷素生物有效性和微生物群落结构的影响 [J]. 应用生态学报, 2023, 34(11): 3030-3038.
[9]	王美娥, 丁寿康, 郭观林, 李勖之, 姜瑢, 张媛, 谢天. 污染场地土壤生态风险评估研究进展 [J]. 应用生态学报, 2020, 31(11): 3946-3958.
[10]	施翔, 王树凤, 陈益泰, 徐琴娣, 孙海菁, 安然, 路晓宏, 路燕, 樊岁君. 5种栎树幼苗对铅锌尾矿砂的耐性与植被恢复前景 [J]. 应用生态学报, 2019, 30(12): 4091-4098.
[11]	杨晓宇, 李程, 马思跃, 李建恒. 添加物及植物组合对瞿麦幼苗铅吸收、生长及品质的影响 [J]. 应用生态学报, 2017, 28(4): 1155-1160.
[12]	季一诺,赵志忠*,吴丹,伏箫诺. 海南东寨港红树林沉积物中重金属的分布及其生物有效性 [J]. 应用生态学报, 2016, 27(2): 593-600.
[13]	李宁1,郭雪雁2,陈世宝1**,刘彬1,宋文恩1. 基于大麦根伸长测定土壤Pb毒性阈值、淋洗因子及其预测模型 [J]. 应用生态学报, 2015, 26(7): 2177-2182.
[14]	谢运河1,2,3,纪雄辉1,2,3,吴家梅1,2,3,黄涓2,3,官迪1,3,朱坚1,2,3. 不同有机肥对土壤镉锌生物有效性的影响 [J]. 应用生态学报, 2015, 26(3): 826-832.
[15]	曹小闯1,2,吴良欢2,3**,马庆旭2,3,金千瑜1. 高等植物对氨基酸态氮的吸收与利用研究进展 [J]. 应用生态学报, 2015, 26(3): 919-929.