Effect of enhanced ammonium nutrition on photosynthesis and nitrate reductase and glutamine synthetase activities of winter wheat

Abstract

Abstract: Acontrolled hydroponics experiment was conducted to investigate the effects of three NH₄⁺/NO₃^- ratios (0/100, 50/50 and 100/0) on the photosynthesis and the key nitrogen metabolism enzymes of three wheat cultivars with different sensitivity to enhanced ammonium nutrition (EAN).Compared with NO₃^- alone, EANsignificantly increased the leaf chlorophyll content, net photosynthetic rate, and soluble sugar content.It also significantly increased the soluble protein content in leaves and roots and the nitrate reductase activity in leaves, but had no significant effect on Glutamine synthetase (GS) activity.EANincreased the soluble sugar content in leaves, and correspondingly, enhanced the net photosynthetic rate and maintained a higher soluble sugar/protein in leaves and roots, which was favorable to the nitrogen assimilation and plant growth.

Key words: Wheat, Enhanced ammonium nutrition (EAN), Net photosynthetic rate, Soluble sugar, Nitrate reductase (NR)

CLC Number:

Q945.11

DAI Tingbo, CAO Weixing, SUN Chuanfan, JIANG Dong, JING Qi . Effect of enhanced ammonium nutrition on photosynthesis and nitrate reductase and glutamine synthetase activities of winter wheat[J]. Chinese Journal of Applied Ecology, 2003, (9): 1529-1532.

References

[1] Alfoldi Z, Pinter L. 1992. Accumulation and partitioning of biomass and soluble carbohydrates in maize seedings as affected by source of nitrogen, nitrogen concentration, and cultivar. J Plant Nutr, 15 (11): 2567～2583
[2] Camberato JJ, Bock BR. 1990. Spring wheat response to enhanced ammonium supply I. Dry matter and N content. Agron J,82:463～467
[3] Cramer MD, Lewis OAM. 1993. The influence of NO₃^- and NH₄⁺ nutrition on the carbon and nitrogen partitioning characteristics of wheat and maize plants. Plant Soil, 154 : 289～300
[4] Dai T-B(戴廷波), Cao W-X(曹卫星). 2000. Promoting effects of enhanced ammonium nutrition on growth of wheat at early stage. J Triticeae Crops(麦类作物学报), 20(4) :42～46 (in Chinese)
[5] Heberer JA, Below FE. 1989. Mixed nitrogen nutrition and productivity of wheat grown in hydroponics. Ann Bot, 63: 643～649
[6] Ji S-Q(季书勤),Zhao S-Z(赵淑章), Wang S-Z(王绍中), et al.1998. The preliminary study on populations quality indexes in Wenmai 6 wheat with yield of 9 000 kg per hm². Acta Agric Sin (中国农业科学), 24(6):865～869(in Chinese)
[7] Lin Y-K(林炎坤). 1989. Comparison and improvement of several common anthrone-colorimetric methods for sugar content in plant.Plant Physiol Commun (植物生理学通讯), 25(4): 53～55(in Chinese)
[8] Magalhaes JR, Huber DM. 1991. Response of ammonium assimilation enzymes to nitrogen form treatments in different plant species.J Plant Nutr, 14(2): 175～185
[9] Pilbeam DJ, Kirkby EA. 1992. Some aspects of the utilization of nitrate and ammonium by plants. In: Mengel K, Pilbeam DJ, eds.Nitrogen Metabolism of Plants. Oxford: Clarendon Press. 55～70
[10] Raab KT, Terry N. 1994. Nitrogen sources regulation of growth and photosynthesis in Beta vulgaris L. Plant Physiol, 105: 1159～1166
[11] Sagi M, Dovrat A, Kipnis T, et al. 1998. Nitrate reductase, phosphoenolpyruvate carboxylase, and Glutamine synthetase in annual ryegrass as affected by salinity and nitrogen. J Plant Nutr, 21 (4):707～723
[12] Salsac LS, Morot-Gaudry CJ. 1987. Nitrate and ammonium nutrition in plants. Plant Physiol Biochem, 25:805～812
[13] Villa MS, Gonzalez GA, Torres JLT, et al. 1992. Effect of the NH₄⁺/NO₃^- ratio on GS and PEPcase activities and on dry matter production in wheat. J Plant Nutr, 15(11):2545～2557
[14] Wang X, Below FE. 1992. Root growth, nitrogen uptake, and tillering of wheat induced by mixed-nitrogen source. Crop Sci, 32:997～1002

[1]	GUO Wei, WANG Nan, ZHANG Kaiyue, SONG Peipei, MA Zhanqiang. Effects of Cu₂O nanoparticles on the kinetic characteristics of rapid chlorophyll fluorescence induction and related genes in wheat seedlings [J]. Chinese Journal of Applied Ecology, 2024, 35(3): 721-730.
[2]	CAI Hongmei, WANG Feifei, WANG Pengna, TANG Zhiwei, HUANG Weixiang, ZHENG Baoqiang, LI Jincai, CHEN Xiang. Research progress on the method and index evaluating strong seedlings of winter wheat during overwintering stage [J]. Chinese Journal of Applied Ecology, 2024, 35(2): 555-563.
[3]	LI Changqing, JI Meng, MA Mengmeng, WANG Shuo, LIU Huan, SUN Zhimei. Effects of combined natural synergists and chemical inhibitors on yield, nitrogen utilization and balance in wheat/maize rotation system [J]. Chinese Journal of Applied Ecology, 2023, 34(9): 2391-2397.
[4]	YANG Yixuan, CHEN Yingzhi, TANG Peng, LIN Wen, SUN Min, GAO Zhiqiang. Effects of sowing patterns on nitrogen utilization and yield formation of winter wheat in the western Huang-Huai-Hai region [J]. Chinese Journal of Applied Ecology, 2023, 34(6): 1572-1582.
[5]	ZHANG Pengfei, LIU Pengzhao, WANG Chenglong, DENG Mingzhu, LIN Yanrong, REN Xiaolong, CHEN Xiaoli. Effects of elevated atmospheric ammonia concentration on photosynthetic characteristics and grain yield in wheat under different nitrogen rates [J]. Chinese Journal of Applied Ecology, 2023, 34(4): 1009-1014.
[6]	LIU Akang, MA Ruiqi, WANG Demei, WANG Yanjie, YANG Yushuang, ZHAO Guangcai, CHANG Xuhong. Effect of accumulated temperature before overwintering on wheat seedling growth status in north winter wheat area of China. [J]. Chinese Journal of Applied Ecology, 2023, 34(3): 679-687.
[7]	HUI Kaishan, WU Zhaohan, ZHANG Yongli. Effects of phosphorus application rates on photosynthetic and senescence characteristics and yield of diffe-rent stems and tillers of wheat under water-saving supplementary irrigation [J]. Chinese Journal of Applied Ecology, 2023, 34(2): 451-462.
[8]	XIE Yongkai, SONG Jinyao, LIU Min, MENG Wanzhong, FENG Meichen, WANG Chao, YANG Wude, QIAO Xingxing, YANG Chenbo. Hyperspectral monitoring on proline content in winter wheat under water stress [J]. Chinese Journal of Applied Ecology, 2023, 34(2): 463-470.
[9]	CHEN Yue, ZHAO Gengxing, CHANG Chunyan, WANG Zhuoran, LI Yinshuai, ZHAO Huansan, ZHANG Shuwei, PAN Jingrui. Grain yield estimation of wheat-maize rotation cultivated land based on Sentinel-2 multi-spectral image: A case study in Caoxian County, Shandong, China [J]. Chinese Journal of Applied Ecology, 2023, 34(12): 3347-3356.
[10]	CUI Guoji, WANG Chuanwei, HE Wei, LI Yuxing, HUANG Zhenglai, ZHANG Wenjing, MA Shangyu, FAN Yonghui. Effects of exogenous trehalose on filling characteristics and sugar component content of wheat under high temperature stress during the filling period. [J]. Chinese Journal of Applied Ecology, 2023, 34(11): 3021-3029.
[11]	LI Chuan-liang, YU Zhen-wen, ZHANG Juan, ZHANG Yong-li, SHI Yu. Effects of nitrogen application rate on the accumulation of ¹³C assimilates after flowering and water-nitrogen use efficiency of wheat under supplemental irrigation based on soil moisture [J]. Chinese Journal of Applied Ecology, 2023, 34(1): 92-98.
[12]	WANG Jie, HUA Yi-fan, QIN Ji-yuan, HE Ming-rong, JU Zheng-chun, LYU Peng, DENG Shu-zhen, DAI Xing-long. Effects of the timing of epibrassinolide spraying on yield and nitrogen use efficiency of wide-belt sowing wheat [J]. Chinese Journal of Applied Ecology, 2023, 34(1): 99-106.
[13]	KONG Ling-ying, ZHAO Jun-ye, ZHANG Zhen, SHI Yu, YU Zhen-wen. Effects of planting density on photosynthetic characteristics of flag leaf and senescence of leaf and root under wide-width sowing condition [J]. Chinese Journal of Applied Ecology, 2023, 34(1): 107-113.
[14]	LI Ying-hao, WANG Qi, ZHAO Bao-ping, MI Jun-zhen, LIU Yan-di, ZHANG Zhi-fen, LIU Jing-hui. Effects of moisture and humic acid on the metabolism of oat fructans [J]. Chinese Journal of Applied Ecology, 2022, 33(5): 1320-1330.
[15]	MA Yu-zhao, DANG Hong-kai, LI Ke-jiang, ZHENG Chun-lian, CAO Cai-yun, ZHANG Jun-peng, LI Quan-qi. Effects of brackish water irrigation on grain quality characteristics and yield of winter wheat [J]. Chinese Journal of Applied Ecology, 2022, 33(4): 1063-1068.