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Low dose benzo(a)pyrene up-regulated the transcription of HSP70 and HSP90 in Eisenia fetida. 

ZHENG Sen-lin1,2,3; SUN Tie-heng1; XIAO Hong1; QIU Xiao-yan4; SONG Yu-fang1    

  1. 1Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110016, China; 2Graduate University of Chinese Academy of Sciences, Beijing 100049, China; 3Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, Fujian, China; 4School of Bioengineering, Jimei University, Xiamen 361021, Fujian, China

  • Received:2007-04-03 Revised:1900-01-01 Online:2008-02-21 Published:2008-02-21

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Abstract: To search for the molecular biomarkers of sub-lethal polycyclic aromatic hydrocarbons (PAHs)-contamination of soil, the subtractive cDNA libraries of earthworm Eisenia fetida exposed to benzo(a)pyrene (BaP) in artificial soil were constructed by suppression subtractive hybridization. After sequencing and analyzing with basic local alignment search tool (BLAST), two clones matching heat shock protein 70 k Da (HSP70) and one clone matching heat shock protein 90 k Da (HSP90) were isolated from the upregulated library, and subsequently, the up-regulation of HSP70 and HSP90 was verified by real-time PCR in E. fetida exposed to 0.1 mg·kg-1 and 1.0 mg·kg-1 BaP. It was indicated that these two newly identified HSPs in E. fetida were the potential molecular biomarkers for soil contamination monitoring.

Key words: re-watering, compensation effect, drought stress, source-sink