Abstract: The study on the effects of different concentration (0, 10，50, 100, 200 and 400 μmol·L^-1) cadmium (Cd) on the antioxidative system in Ganoderma lucidum mycelia indicated that with increasing concentration of Cd, the fresh mass and the proline, total polysaccharides, and reduced polysaccharides contents of G. lucidum mycelia decreased, but non-protein thiol (NPT) content increased. At 400 μmol·L^-1 of Cd, the NPT content increased dramatically, being 5.7 times higher than control. Within the range of test Cd concentrations, the activities of CAT, GR and POD increased first and decreased then, with the peak at 100 μmol·L^-1 of Cd, while the activities of LOX and SOD increased with increasing Cd concentration, with the maximum at 400 μmol·L^-1 of Cd. Polyacrylamide gel electrophoresis analysis revealed that 100-400 μmol·L^-1 of Cd induced two additional isozymes bands of Mn-SOD, 10-200 μmol·L^-1 of Cd increased the intensity of constitutive isozymes of CAT, POD, SOD and LOX, while 400 μmol·L^-1 of Cd decreased the intensity of isozymes of POD significantly.

Key words: rotation, Loess Plateau, wheat, protein quality, nutritional quality.