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Chinese Journal of Applied Ecology ›› 2009, Vol. 20 ›› Issue (10): 2406-2410.

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Difference in seedlings ammonium assimilation of wheat cultivars with different drought resistance under osmotic stress

ZHANG Hui-na|WANG Zhi-qiang|CUI Guo-jin|LIN Tong-bao   

  1. Ministry of Education Key Laboratory for Crop Growth and Development Regulation, College of Agronomy, Henan Agricultural University, Zhengzhou 450002, China
  • Online:2009-10-19 Published:2009-10-19

Abstract: Taking wheat cultivars drought-resistant Luohan-6 and drought-sensitive Zhoumai-18 as test objects, their seedlings ammonium assimilation enzyme activities and related parameters were determined under osmotic stress. The plant biomass had an obvious decrease under osmotic stress, with a larger decrement for Zhoumai-18 than Luohan-6. Osmotic stress increased the plant ammonium content, especially for Zhoumai-18. The glutamine synthetase (GS) activity varied with wheat cultivars. For Luohan-6, the GS activity increased significantly under low osmotic stress but decreased under high osmotic stress; while for Zhoumai-18, the GS activity decreased with increasing osmotic stress. The NADH-dependent glut
amate dehydrogenase (NADH-GDH) increased with increasing osmotic stress, with a marked increment under low osmotic stress for Zhoumai-18, and under high osmotic stress for Luohan-6. The NAD+-dependent glutamate dehydrogenase (NAD+-GDH) and NADP-dependent isocitrate dehydrogenase (NADP-ICDH) activities also increased with increasing osmotic stress, with a greater increment of NAD+-GDH activity for Zhoumai-18, and of NADP-ICDH activity for Luohan-6. It was suggested that the increased drought resistance of wheat plants could be related to the incre
ased ammonium assimilation resulted from the enhanced GS and NADH-GDH activities under low and high osmotic stress, respectively.

Key words: wheat, drought resistance, osmotic stress, ammonium assimilation, soil organic carbon, wavelet transform, multi-scale correlation, partial correlation coefficient,  mountainous area of Guangdong Province.