基于脂肪酸和稳定同位素的东海剑尖枪乌贼摄食生态季节差异

doi:10.13287/j.1001-9332.202307.017

应用生态学报 ›› 2023, Vol. 34 ›› Issue (7): 1754-1762.doi: 10.13287/j.1001-9332.202307.017

• 稳定同位素生态学专栏 • 上一篇下一篇

基于脂肪酸和稳定同位素的东海剑尖枪乌贼摄食生态季节差异

郭荣佩¹, 张柏豪¹, 李楠¹, 方舟^1,2,3,4,5*

¹上海海洋大学海洋科学学院, 上海 201306;
²大洋渔业资源可持续开发教育部重点实验室, 上海 201306;
³国家远洋渔业工程技术研究中心, 上海 201306;
⁴农业农村部大洋渔业开发重点实验室, 上海 201306;
⁵农业农村部大洋渔业资源环境科学观测实验站, 上海 201306

收稿日期:2023-03-23 接受日期:2023-05-18 出版日期:2023-07-15 发布日期:2024-01-15
通讯作者: *E-mail: zfang@shou.edu.cn
作者简介:郭荣佩, 女, 1999年生, 硕士研究生。主要从事头足类繁殖生物学研究。E-mail: 358724545@qq.com
基金资助:
国家重点研发计划项目(2019YFD0901404)和国家自然科学基金面上项目(NSFC41876141)

Seasonal differences of feeding ecology of Uroteuthis edulis in the East China Sea based on fatty acid and stable isotope

GUO Rongpei¹, ZHANG Baihao¹, LI Nan¹, FANG Zhou^1,2,3,4,5*

¹College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China;
²Ministry of Education Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Shanghai 201306, China;
³National Engineering Research Center for Oceanic Fisheries, Shanghai 201306, China;
⁴Key Laboratory of Ocean Fisheries Exploitation, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China;
⁵Scientific Observing and Experimental Station of Oceanic Fishery Resources, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China

Received:2023-03-23 Accepted:2023-05-18 Online:2023-07-15 Published:2024-01-15

摘要/Abstract

摘要： 剑尖枪乌贼是东海重要的渔业资源捕捞对象,同时也在东海食物网中占据重要地位。本研究根据2020年9月至2021年1月在东海采集的剑尖枪乌贼样本,利用脂肪酸标记法和稳定同位素技术对其在秋冬季的摄食生态差异进行研究。结果表明: 秋冬季的剑尖枪乌贼脂肪酸均是多不饱和脂肪酸(PUFA)的含量最高,其次为饱和脂肪酸(SFA),而单不饱和脂肪酸(MUFA)的含量最低。秋季和冬季的PUFA和MUFA含量均存在显著差异,而SFA含量则无明显差异。非度量多维尺度分析表明,油酸(C18:1n9)可作为秋季样本的特征脂肪酸,二十二碳六烯酸(C22:6n3)可作为冬季样本的特征脂肪酸。稳定同位素分析表明,秋季和冬季的δ¹⁵N存在显著差异,而δ¹³C则无显著差异;秋季样本的营养生态位总面积(TA)、δ¹⁵N范围(NR)、标准椭圆面积(SEA)和校正的标准椭圆面积(SEA_C)均小于冬季,而δ¹³C范围(CR)则相反。Spearman秩相关性检验结果显示,秋季和冬季的剑尖枪乌贼的脂肪酸含量和稳定同位素比值与个体胴长之间的相关性存在差异。本研究结果为东海食物网的物流能流研究提供了基础数据,有利于剑尖枪乌贼资源的可持续开发利用。

关键词: 东海, 剑尖枪乌贼, 脂肪酸, 稳定同位素, 摄食生态

Abstract: Uroteuthis edulis, an important fishery target species, plays an important role in the food web of the East China Sea. We collected U. edulis samples in the East China Sea from September 2020 to January 2021 to examine their feeding differences in autumn and winter based on fatty acid and stable isotope analyses. The results showed that the content of polyunsaturated fatty acids (PUFA) was the highest, followed by saturated fatty acids (SFA), and the lowest content of monounsaturated fatty acids (MUFA) in autumn and winter. Results of the similarity ana-lysis showed significant differences in PUFA and MUFA contents but no differences in SFA contents between autumn and winter. Results of non-metric multidimensional scale analysis showed that C18:1n9 could be used as signature fatty acids in autumn samples and C22:6n3 as characteristic fatty acids in winter samples. There was significant difference of δ¹⁵N between autumn and winter, but no difference of δ¹³C. The total area (TA), range of δ¹⁵N (NR), standard ellipse area (SEA) and the corrected version of the standard ellipse area (SEA_C) in autumn were all smaller than those in winter, but range of δ¹³C (CR) was on the contrary. Results of the Spearman rank correlation test showed that there were differences between fatty acid content and stable isotope ratio of U. edulis and the dorsal mantle length in autumn and winter. Our results could provide basic data for understanding material and energy flow of the East China Sea food web, which is conducive to the sustainable development and utilization of U. edulis.

Key words: East China Sea, Uroteuthis edulis, fatty acid, stable isotope, feeding ecology

郭荣佩, 张柏豪, 李楠, 方舟. 基于脂肪酸和稳定同位素的东海剑尖枪乌贼摄食生态季节差异[J]. 应用生态学报, 2023, 34(7): 1754-1762.

GUO Rongpei, ZHANG Baihao, LI Nan, FANG Zhou. Seasonal differences of feeding ecology of Uroteuthis edulis in the East China Sea based on fatty acid and stable isotope[J]. Chinese Journal of Applied Ecology, 2023, 34(7): 1754-1762.

参考文献

[1] 陈新军, 刘必林, 王尧耕. 世界头足类. 北京: 海洋出版社, 2009
[2] 李楠, 方舟, 陈新军. 东海北部外海剑尖枪乌贼渔业生物学特征. 上海海洋大学学报, 2020, 29(4): 622-631
[3] 丁天明, 宋海棠. 东海剑尖枪乌贼生物学特征. 浙江海洋学院学报: 自然科学版, 2000, 19(4): 371-374
[4] Caddy JF, Rodhouse PG. Cephalopod and groundfish landings: Evidence for ecological change in global fishe-ries? Review in Fish Biology and Fisheries, 1998, 8: 431-444
[5] 郑元甲. 东海区头足类资源开发利用现状及合理利用. 海洋渔业, 1994(5): 199-203, 200
[6] Pierce GJ, Boyle PR, Hastie LC, et al. Diets of squid Loligo forbesi and Loligo vulgaris in the northeast Atlantic. Fisheries Research, 1994, 21: 149-163
[7] 李楠, 方舟, 陈新军. 剑尖枪乌贼渔业研究进展. 大连海洋大学学报, 2020, 35(4): 637-644
[8] Mintzer V, Gannon D, Barros N, et al. Stomach contents of mass-stranded short-finned pilot whales (Globicephala macrorhynchus). Marine Mammal Science, 2008, 24: 290-302
[9] Perissinotto R, Pakhomov EA. Feeding association of the copepod Rhincalanus gigas with the tunicate salp Salpa thompsoni in the southern ocean. Marine Biology, 1997, 127: 479-483
[10] 沈亚男, 张博, 姜松, 等. 基于脂肪酸标志法的不同海区合浦珠母贝的摄食差异研究. 南方水产科学, 2015, 11(6): 27-33
[11] Lajtha K, Michener RH. Stable isotopes in ecology and environmental science. Freshwater Biology, 2010, 54: 434-435
[12] 王峥, 刘长琳, 翟介明, 等. 莱氏拟乌贼肌肉营养成分分析及评价. 渔业科学进展, 2020, 41(4): 102-109
[13] 张宇美. 基于碳氮稳定同位素的南海鸢乌贼摄食生态与营养级研究. 硕士论文. 湛江: 广东海洋大学, 2014
[14] 刘长琳, 阮飞腾, 秦搏, 等. 野生金乌贼成体肌肉的营养成分分析及评价. 海洋科学, 2016, 40(8): 42-48
[15] Parry M. Trophic variation with length in two ommastrephid squids, Ommastrephes bartramii and Sthenoteuthis oualaniensis. Marine Biology, 2008, 153: 249-256
[16] Ruiz-Cooley RI, Markaida U, Gendron D, et al. Stable isotopes in jumbo squid (Dosidicus gigas) beaks to estimate its trophic position: Comparison between stomach contents and stable isotopes. Journal of the Marine Biological Association of the United Kingdom, 2006, 86: 437-445
[17] 赵振方, 胡贯宇, 陈新军, 等. 秘鲁外海茎柔鱼脂肪酸组成的月间差异研究. 海洋湖沼通报, 2022, 44(4): 98-105
[18] 谢嘉仪, 张丽姿, 吴文秀, 等. 南沙群岛海域鸢乌贼摄食习性与营养生态位. 水产学报, 2021, 45(12): 1993-2002
[19] McKinney CR, McCrea JM, Epstein S, et al. Improvements in mass spectrometers for the measurement of small differences in isotope abundance ratios. Review of Scientific Instruments, 1950, 21: 724-730
[20] Layman C, Arrington D, Montana C, et al. Can stable isotope ratios provide quantitative measures of trophic diversity within food webs? Ecology, 2007, 88: 42-58
[21] 高春霞. 基于稳定同位素技术的浙江中南部近海渔业生物群落营养结构研究. 博士论文. 上海: 上海海洋大学, 2020
[22] Jackson AL, Inger R, Parnell AC, et al. Comparing isotopic niche widths among and within communities: SIBER-Stable Isotope Bayesian Ellipses in R. Journal of Animal Ecology, 2011, 80: 595-602
[23] Parrish CC, Abrajano TA, Budge SM, et al. Lipid and phenolic biomarkers in marine ecosystems: Analysis and applications// Wangersky PJ, ed. Marine Chemistry. Berlin: Springer, 2000: 193-223
[24] Rajendran N, Suwa Y, Urushigawa Y. Distribution of phospholipid ester-linked fatty acid biomarkers for bacteria in the sediment of Ise Bay, Japan. Marine Chemistry, 1993, 42: 39-56
[25] Pond DW, Bell MV, Harris RP, et al. Microplanktonic polyunsaturated fatty acid markers: A mesocosm trial. Estuarine, Coastal and Shelf Science, 1998, 46: 61-67
[26] Johns RB, Nichols PD, Perry GJ. Fatty acid composition of ten marine algae from Australian waters. Phytochemistry, 1979, 18: 799-802
[27] Parrish CC. Determination of total lipid, lipid classes, and fatty acids in aquatic samples// Arts MT, Wainman BC, eds. Lipids in Freshwater Ecosystems. New York: Springer, 1998: 5-20
[28] Persson J, Vrede T. Polyunsaturated fatty acids in zooplankton: Variation due to taxonomy and trophic position. Freshwater Biology, 2006, 51: 887-900
[29] Khalaf MA, Kochzius M. Change in trophic community structure of shore fishes at an industrial site in the Gulf of Aqaba, Red Sea. Marine Ecology Progress Series, 2002, 241: 287-299
[30] 李云凯, 汪惠琼, 陈新军, 等. 柔鱼科近缘种茎柔鱼与鸢乌贼营养生态位及相互关系. 生态学报, 2020, 40(15): 5418-5423
[31] Food and Agriculture Organization of the United Nations/World Health Organization (FAO/WHO). Fats and Oils in Human Nutrition. Report of a Joint Expert Consultation. Rome: Food and Agriculture Organization, 1994: 49-55
[32] 高淳仁, 雷霁霖. 海水鱼类高度不饱和脂肪酸营养研究概况. 海洋水产研究, 2000, 21(3): 72-75
[33] 林静远, 刘必林, 桓梦瑶, 等. 我国几种常见头足类角质颚碳氮稳定同位素分析. 上海海洋大学学报, 2019, 28(3): 394-401
[34] Cherel Y, Fontaine C, Jackson GD, et al. Tissue, ontogenic and sex-related difference in δ¹³C and δ¹⁵N values of the oceanic squid Todarodes filippovae (Cephalopoda: Ommastrephidae). Marine Biology, 2009, 156: 699-708
[35] 李云凯, 贡艺, 陈新军. 稳定同位素技术在头足类摄食生态学研究中的应用. 应用生态学报, 2014, 25(5): 1541-1546
[36] 张硕, 谢斌, 符小明, 等. 应用稳定同位素技术对海州湾拖网渔获物营养级的研究. 海洋环境科学, 2016, 35(4): 507-511
[37] 戴芳群, 朱玲, 陈云龙. 黄、东海渔业资源群落结构变化研究. 渔业科学进展, 2020, 41(1): 1-10
[38] Ruyter B, Rojo C, Grisdale-Helland B, et al. Influence of temperature and high dietary linoleic acid content on esterification, elongation, and desaturation of PUFA in Atlantic salmon hepatocytes. Lipids, 2003, 38: 833-840
[39] 贡艺, 陈新军, 李云凯, 等. 秘鲁外海茎柔鱼摄食洄游的稳定同位素研究. 应用生态学报, 2015, 26(9): 2874-2880
[40] Li N, Han PW, Wang C, et al. Migration routes of the swordtip squid Uroteuthis edulis in the East China Sea determined based on the statolith trace element information. Hydrobiologia, 2023, 850: 861-880
[41] 纪炜炜. 东海中北部主要游泳动物食物网结构和营养关系初步研究. 博士论文. 青岛: 中国科学院海洋研究所, 2011
[42] 张琥顺, 宋普庆, 郑新庆, 等. 基于稳定同位素方法的东海中南部海域主要饵料生物营养生态位季节变动的研究. 应用海洋学学报, 2022, 41(3): 375-385
[43] 姜亚洲, 林楠, 袁兴伟, 等. 基于碳、氮稳定同位素技术研究象山港虾虎鱼类营养生态位. 生态学杂志, 2015, 34(6): 1579-1585
[44] 纪炜炜, 李圣法, 陈雪忠. 鱼类营养级在海洋生态系统研究中的应用. 中国水产科学, 2010, 17(4): 878-887

基于脂肪酸和稳定同位素的东海剑尖枪乌贼摄食生态季节差异

Seasonal differences of feeding ecology of Uroteuthis edulis in the East China Sea based on fatty acid and stable isotope

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