两个基因型水稻利用有机磷的差异及其与根系分泌酸性磷酸酶活性的关系

应用生态学报

两个基因型水稻利用有机磷的差异及其与根系分泌酸性磷酸酶活性的关系

李永夫^{1, 2};罗安程²;吴良欢²;魏兴华³

¹浙江林学院环境科技学院，浙江临安 311300;²浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室，杭州 310029;³中国水稻研究所水稻生物学国家重点实验室，杭州 310006

收稿日期:2008-10-13 修回日期:1900-01-01 出版日期:2009-05-20 发布日期:2009-05-20

Difference in P utilization from organic phosphate between two rice genotypes and its relations with root-secreted acid phosphatase activity.

LI Yong-fu^1,2;LUO An-cheng²;WU Liang-huan²;WEI Xing-hua³

¹School of Environmental Science and Technology, Zhejiang Forestry University, Lin’an 311300, Zhejiang, China;²Ministry of Education Key Laboratory of Environmental Remediation and Ecosystem Health, College of Environmental and Resource Science, Zhejiang University, Hangzhou 310029, China;³State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China

Received:2008-10-13 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20

摘要/Abstract

摘要： 以低磷条件下根系分泌酸性磷酸酶活性有显著差异的两个基因型水稻中部51和Azucena为材料，通过琼脂培养试验研究它们在无菌条件下利用植酸钠（IHP）的情况以及接种土壤微生物对水稻利用植酸钠能力的影响.结果表明：以植酸钠为磷源生长的中部51和Azucena的植株地上部干物质量、吸磷量和磷浓度均显著低于以无机磷为磷源生长的植株，植酸钠处理的水稻植株地上部吸磷量和磷浓度也均显著高于无磷处理植株，表明无菌培养条件下水稻能部分利用植酸钠.低磷条件下两个基因型水稻根系分泌酸性磷酸酶活性显著高于正常供磷处理，且低磷条件下中部51根系分泌的酸性磷酸酶活性较高，这可能是无菌条件下中部51利用植酸钠的能力高于Azucena的机理之一.高水平植酸钠处理（0.96 mmol P·L^-1）下两个基因型水稻植株地上部干物质量、磷含量及磷浓度均显著高于低水平植酸钠处理（0.16 mmol P·L^-1），表明底物有效性可能是影响水稻利用植酸钠能力的限制因素之一.在低水平和高水平植酸钠处理下，接种土壤微生物对两个基因型水稻植株的地上部干物质量、磷含量及磷浓度均没有显著影响，表明在本试验中接种土壤微生物并不能显著提高水稻利用植酸钠的能力.

关键词: 长期施肥, 红壤水稻土, 土壤有机碳, 颗粒有机碳, KMnO₄氧化C, 固碳量

Abstract: With two rice genotypes (cv. Zhongbu 51 and Azucena) differing in their root-secreted acid phosphatase activity (APA) under low P condition as test materials, an agar culture experiment was conducted to investigate their P utilization from myo-inositol hexaphosphate (IHP) under conditions of sterilization and inoculating with soil microorganisms. Under sterilization, the shoot dry mass, P absorption and P concentration of test genotypes supplied with hexaphosphate (IHP) as the P source were significantly higher than those with no P supply, but significantly lower than those supplied with inorganic P, indicating that rice plants could partly acquire P from IHP under sterilized condition. The root-secreted APA of the two genotypes was significantly higher under low P than under sufficient P supply, and the higher APA of Zhongbu 51 under low P could be one of the mechanisms of higher P utilization capability of Zhongbu 51 than Azucena under deficient P supply. When supplied with high level IHP (0.96 mmol P·L-1), the shoot dry mass, P absorption and P concentration of both Zhongbu 51 and Azucena were higher than those when supplied with low level IHP (0.16 mmol P·L-1), indicating that substrate availability could be one of the limiting factors to the utilization of phytate by rice. Inoculation with soil microorganisms had no significant effects on the shoot dry mass, P absorption and P concentration of rice plants, regardless of IHP level and rice genotypes, implying that the utilization of IHP by rice could not be improved via inoculation with soil microorganisms.

Key words: long-term fertilization, reddish paddy soil, soil organic C, particulate organic C, KMnO₄C, carbon sequestration rate.

李永夫^{1, 2};罗安程²;吴良欢²;魏兴华³. 两个基因型水稻利用有机磷的差异及其与根系分泌酸性磷酸酶活性的关系[J]. 应用生态学报.

LI Yong-fu^1,2;LUO An-cheng²;WU Liang-huan²;WEI Xing-hua³. Difference in P utilization from organic phosphate between two rice genotypes and its relations with root-secreted acid phosphatase activity.[J]. Chinese Journal of Applied Ecology.

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