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应用生态学报 ›› 2009, Vol. 20 ›› Issue (09): 2118-2122.

• 研究报告 • 上一篇    下一篇

接种丛枝菌根对不同施氮水平下黄芪生理特性和营养成分的影响

贺学礼;刘媞;赵丽莉   

  1. 河北大学生命科学学院| 河北保定 |071002
  • 收稿日期:2009-01-12 出版日期:2009-09-20 发布日期:2009-09-20

Effects of inoculating AM fungi on physiological characters and nutritional components of Astragalus membranaceus under different N application levels.

HE Xue-li;LIU Ti;ZHAO Li-li   

  1. College of Life Sciences, Hebei University, Baoding 071002, Hebei, China
  • Received:2009-01-12 Online:2009-09-20 Published:2009-09-20

摘要: 以非灭菌土为生长基质,采用盆栽试验,研究了不同施氮水平下接种丛枝菌根(AM)真菌对黄芪生理特性和营养成分的影响.结果表明: AM真菌对不同施氮水平下黄芪的生长效果有显著影响,接种AM真菌能够提高黄芪根系菌根侵染率,但高施氮量抑制了AM真菌对黄芪根系的侵染.不同施氮水平下接种AM真菌可使黄芪生长量、叶片可溶性蛋白、可溶性糖含量及SOD、POD和CAT活性有不同程度的提高,且50 mg N·kg-1土和100 mg N·kg-1土施氮水平下接种株POD同工酶出现了新谱带,接种AM真菌对黄芪植株的黄酮、N和P含量也有一定促进作用.施氮量为50~100 mg N·kg-1土时接种效果最佳.

关键词: AM真菌, 施氮量, 营养成分, 生理特性, 黄芪, 硝化抑制剂, 双氰胺, 作用机理, 抑制效果, 氮肥利用率

Abstract: A pot culture with unsterilized soil as growth substrate showed that AM fungi had significant effects on the growth of Astragalus membranaceus (Fabaceae) under different N application levels. Inoculation with AM fungi promoted the AM infection of A. membranaceus roots, but high N application level suppressed the infection. Inoculating AM fungi increased the growth rate, soluble protein and sugar contents, and SOD, POD and CAT activities of A. membranaceus. Under 50 and 100 mg·kg-1 of N application, new bands of POD isozyme occurred in inoculated plants, and the contents of flavonoid, N, and P in A. membranaceus also had definite increase. The best inoculation effect was observed under the N application level of 50〖KG-*2〗-〖KG-*7〗100 mg N·kg-1 soil.

Key words: AM fungi, N application level, nutritional component, physiological character, Astragalus membranaceu, nitrification inhibitor, dicyandiamide (DCD), inhibition mechanism, inhibition effect, nitrogen fertilizer efficiency.